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The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明：盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深，这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明：在盐酸胍溶液中，随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深，其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多，直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基，在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个，而随着其分子变性程度的进一步加深，反而减少至5个。

A few first bleached little primroses too, by the path, and yellow buds unfolding themselves.

在小径的旁边，还有些快出的小莲馨花，乍开着黄色的花蕾。

A few first bleached little primroses too, by the path, and yellow buds unfolding themselves.

在小径的旁边，还有些抉出的小莲馨花，乍开着黄色的花蕾。

The grain-like cell inclusions which varied in morphology, location and number during the different periods of unfolding of perianths of Michelia alba DC. can be inferred to be a predecessor of aroma.

结果表明：(1)根据在花被片开展的不同时期的颗粒状细胞内含物的数量、形态及存在部位，推测白兰花被片颗粒状细胞内含物可能上要是香气释放前体物质。

In pursuance of this purpose, I have endeavored to select and group together events in the history of the church in such a manner as to trace the unfolding of the great testing truths that at different periods have been given to the world, that have excited the wrath of Satan, and the enmity of a world-loving church, and that have been maintained by the witness of those who "loved not their lives unto the death."

为了达到这一目的，我选了一些教会历史的史事，将这些大事联贯起来，以便说明一些重大而有考验性的真理在不同的时代是怎样逐步发展，怎样传给世人，因而激起撒但和贪爱世俗的教会仇视这些真理，就是由那些&虽至于死，也不爱惜性命&（启12：11）之人的见证保存下来的。

On the other hand, the existence of intermediate during pH induced unfolding process implied that the conformational transition of RSF does not fit the simple two-state mechanism.

同时我们还发现，在相当宽的pH范围内，丝素蛋白的二级结构存在着中间体形态，表明丝素蛋白的变性过程不符合简单的二态机制。

The treatments of NPK and NPK+BCF were better than those of N, NP, NK on the plant height, stem, leaves area, chlorophyll, bearing fruit stick, extent of unfolding, the time of blooming and bearing fruit, and plant soma changes of harvest time. The ratio of basal manure to after manuring and the different ratio of after manuring showed mutual effects: The excessive nutrient had unfavorable influences on plant growth and development in former time.

NPK+BCF及NPK的配方在株高、茎粗、叶面积、叶绿素含量、结果枝、展开度、开花期和挂果期、收获期植株营养体变化等指标上都优于NP、NK及只施N肥的配方；不同基追肥比例及不同追肥比例表现出共同的效果：前期施入过多的养分不利于茄子植株的生长发育。

AT key moments in sports history, time itself seems to slow down and stare, slack-jawed, at the intense action unfolding on the court or field.

在每一个体育史上的关键时刻，仿佛时间都会放慢脚步，瞪大双眼凝视着这激情瞬间卓然登场。

Unfolding over the course of twenty years, the film chronicles the life of a woman whose self-sacrificial love for a man ultimately consumes her.

改编自茨威格的短片小说，场景从百年前的维也纳移到了中国的战争年代，这是一场不求回报的爱情悲剧，是一个女人历时20年的爱情牺牲与自我毁灭。

With the unfolding of the CPPCC and NPC, we have received thousands of mobile phone messages from our readers, telling their own social frustrations and dissatisfactions.

接下来的这一周，让我们共同来探讨一下有什么样的办法可以解决这些困惑和不如意，让咱老百姓也给政府支支招。

Salt is good, but if the salt becomes flat and tasteless, with what do you season it?

14：33 盐本是好的，盐若失了味，可用什么叫它再咸呢？

He reiterated that the PLA is an army of the people under the leadership of the Communist Party of China.

他重申，人民解放军是在中国共产党领导下的人民军队。