查询词典 unbounded sequence

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

After analyzing the Sp1 DNA sequence within 5'-UTR and promoter region, we found one typical IRES-conserved sequence localizing within 5'-UTR of Sp1 genomic sequence. The reporter assay and polysome distribution assay revealed that Sp1 could be accumulated strongly and rapidly under hypoxia/ischemia neuron cells.

在Polysome distribution assay的结果显示，Sp1在缺糖缺氧之后转译效率有提高的情形，接著分析Sp1 5'-UTR，发现可能存在一个典型的IRES结构，利用bicistronic assay也证实了Sp1的堆积是透过IRES所导致。

The quasi -like sequence method by which the output sequence constructs the input sequence of variable - memory threshold is suggested in this paper.

给出了一种由输出序列构造可变存储器输入序列拟合序列的方法。

The entire theory systems of the punctured binary sequence pair and the punctured binary array pair are built, which include the perfect punctured binary sequence pair theory, the perfect punctured binary array pair theory, the quasi-perfect and double quasi-perfect binary array pair theory, and the punctured binary complementary sequence pair theory.

定义了屏蔽二进序列偶、最佳屏蔽二进序列偶和奇周期最佳屏蔽二进序列偶，研究了它们的变换性质和存在的组合允许条件，并用这些性质和条件，搜索出若干最佳屏蔽二进序列偶和奇周期最佳屏蔽二进序列偶。

The fan delta, sand beach, sand bars and turbidite fan in the TST of SⅡ and SⅢ sequences are the most favorable exploration targets because of favorable source - reservoir - seal assemblages. Since the high - stand system tract in SⅡ sequence has good source - reservoir assemblage and is covered by the regional seal of SⅢ sequence, the hydrocarbon accumulation in this area is controlled by the local seal rock. The HST of SⅡ sequence becomes the second favorable exploration target zone. Because the scales of the sand bodies of alluvial fan and braided river in low - stand system tract of SⅡ and SⅢ sequences are limited, the formation of a large hydrocarbon accumulation is difficult.

良好的生储盖配置使SⅡ层序、SⅢ层序水进体系域的扇三角洲、滩坝及浊积扇砂体成为本区最佳勘探目标层。SⅡ层序高位体系域生储条件配置好，又有SⅢ层序区域盖层的整体封盖，油气聚集受局部盖层直接控制，可成为较有利的勘探目的层。SⅡ层序、SⅢ层序低位体系域的冲积扇、辫状河砂体体积规模较小，难以形成大油气藏。

Methods(1) Construction of plant expression vector containing RS geneThe cloning vector pT-RS was constructed containing RS gene extracted from the leaf tip of Vitis vinifera by PCR amplification and RS sequence was identified by PCR amplification,enzymes digestion and sequence analysis.The plant expression vector containing RS gene was obtained and RS sequence was identified by PCR amplification and double enzymes digestion too.

研究方法(1)含白藜芦醇合酶基因的植物表达载体的构建提取葡萄基因组总DNA，通过PCR扩增得到RS基因，将此基因连接到克隆载体pGEM-T Vector，得到重组载体pT-RS；经PCR、酶切及序列分析鉴定后，将RS基因克隆到植物表达载体pBI121，得到重组载体pBI-RS，用PCR及双酶切方法进行鉴定。

Sequence analysis showed that the BDNF gene of Asiatic Black Bear was highly conserved compared to those of human and giant panda, with an identity of 94.5% and 98.9%, respectively. The deduced amino acid sequence of the mature protein was found to be identical to those of all the reported mammalians. According to gene sequence alignment, the giant panda appeared to be phylogenetically closer to Asiatic Black Bear than the lesser panda.

在推导的多肽序列中，其成熟区氨基酸序列与所有已报道哺乳动物的完全一致；对亚洲黑熊及其相关物种BDNF基因序列的比较分析，发现大熊猫与包括黑熊在内的熊科动物亲缘关系更近，而与小熊猫较远。

Sequence Number field - Indicates the sequence number assigned to the frame; retransmitted frames are identified by duplicate sequence numbers

序列号域－指定分配给帧的序列号；重传帧被标识为相同的序列号

For the case where the firing sequence sets involved are Petri net firing sequence sets we present a method for synthesizing a control strategy by which the closed loop system can generate the supremal controllable subset of a given firing sequence set.

在目标序列集不可控而所考虑的序列集都是Petri网点发序列集时，提出了综合给定序列集的极大可控子序列集生成器的方法。

The problem of a Griffith crack in an unbounded orthotropic functionally graded material subjected to antiplane shear impact was studied.

研究了无限大正交各向异性功能梯度材料Griffith裂纹受反平面剪切冲击作用的问题。

The use of the web to spread fears that flicking the switch on the LHC could create a Black Hole that could swallow up the Earth particularly concerned him, he said.

他说，使用互联网散布恐慌说启动LHC会生成一个可能吞噬地球的黑洞的做法让他感到忧虑。

Not a single word have I written down.

我一个字也没有写下来。