查询词典 unbounded sequence

The BLAST programs were used for searching protein and DNA databases for sequence similarities. The BLAST result showed that it was a new sequence and similar to zebra fish DNA sequence and some fish reverse transcriptase. But it has small homology of CYP 19 gene sequences that have been in Genbank.In addition, sequences were amplified by RT-PCR with using degenerate primers that weredesigned against CYP4 sequences.

另外，还根据昆虫CYP4家族的特异性氨基酸序列及P450超家族的共同特征序列，采用另一对简并引物，同样进行有关研究，结果显示：疣荔枝螺不同组织的RNA中都含有一长468bp左右的序列，这同样是一段新序列，与部分物种的CYP4存在一定的相关性，可能在螺体内起一定重要的作用，但具体功能有待进一步研究。

The elements of each sequence in odd-periodic perfect almost binary sequence pair are "1" or "-1" with exception of a single zero element, and odd-periodic perfect almost binary sequence pair possess a perfect odd-autocorrelation function.

奇周期最佳几乎二进序列偶中2个序列中均有一个元素为"0"元素，其他元素取值均为"＋1"或"－1"，并且其奇周期自相关函数为单值脉冲函数。

Pilot zero sequence directional protection may fail to operate because the zero sequence current on one end does not reach the set value of the protective relay,although zero sequence directional elements on both ends operate correctly.

线路发生接地故障时，两侧零序阻抗相差过大将导致两侧零序电流差别过大，虽然两侧的零序方向元件均能正确动作，但是由于一侧零序电流达不到动作定值，从而造成纵联零序方向保护拒动。

Because of sharing characteristics with both Galliform groups, Tragopans are classified as partridges by some authors and pheasants by some others. In this study we set out to sequence the cytochrome b-gene of 19 species pheasants. and took the sequence for those 22 species from GenBank. We amplified 828 base pairs using the PCR technique. Primers designed by ourselves were based upon Galliform sequence data. Neighbor-joining method and Minimum evolution method were used to analyse cytochrome b sequences with Anhima cornuta and Kachuga dhongoka as outgroups.

通过PCR扩增鸡形目Galliformes黄腹角雉Tragopan caboti等10个属19个个体的线粒体DNA细胞色素b的部分基因，获取片段长度为828bP，以及从GenBank获取22种样本的相应序列，以角叫鸭Anhima cornuta和海龟Kachuga dhongoka为外群，分别用邻接法(Neighbor-joining， NJ)和最小进化法(Minimum-evolution， ME)，对鸡形目和将雉科的15个属分为5个属及雉族和鹑族构建分子系统树，NJ和ME系统树中，都是角雉与雉类相聚。

Analyzing by blastn program of NCBI, the fragment includes: 30bp 12srRNA partial sequence, 71bp tRNA-Val complete sequence and 913bp 16srRNA partial sequence, and the homolog is more than 95 % compared with other Camelidae animals.

PCR条件优化后成功扩增出长度为1014 bp的DNA片段，blastn分析显示，该片断与骆驼科动物的同源性高于95%，所获得片断包括30 bp的12srRNA基因部分序列，71bp的tRNA-Val基因全序列和913bp的16srRNA基因部分序列。

Seven thirdorder seismic sequences can be recognized from bottom to top in the Paleogene in the Wushi sag, which consist of a depositional sequence from transgression to regression as a whole and correspond to different stages of structural evolution. Sequences developed in different stages of structural evolution are different in filling sequence and distribution of sedimentary system. Multiphase structural evolutions result in development of source rocks and reservoir and cap rocks in various parts of the sag. The sequence 2-4 deposited in strong chasmic stage have the best source rocks and sealing conditions as well as various favorable reservoirs, thus are the most favorable targets for obtaining discoveries in this sag.

结果认为：乌石凹陷古近系从下至上可以划分为7个三级地震层序，整体上为一个水进到水退的序列，分别对应不同的构造演化阶段；不同构造演化阶段发育的层序具有不同的充填序列和沉积体系分布；构造演化的多期性使得该凹陷多个部位都有烃源岩和储、盖层发育；其中以强烈裂陷阶段沉积的层序2-层序4中发育的烃源岩和盖层条件为最好且发育多种有利储集体，是该凹陷寻找油气藏的最有利层段。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

By comparition on the recognition of normal and sheared DNA sequence, it can be found that, the intercalation at the mismatch site of sheared DNA sequence more specificially than into normal sequence.

但是由于两种手性配合物的插入能量差异不是太大，实验手段可能无法得到满意结果，这可能是导致国际上其他实验工作组研究结果不一致的原因所在；（3）沟选择性比较明显。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The use of the web to spread fears that flicking the switch on the LHC could create a Black Hole that could swallow up the Earth particularly concerned him, he said.

他说，使用互联网散布恐慌说启动LHC会生成一个可能吞噬地球的黑洞的做法让他感到忧虑。

Not a single word have I written down.

我一个字也没有写下来。