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Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400

The purpose of paper is to describe the petrology character of outcrop sandstones and core sandstones and judge their roke type, compare the similarities and differences of autogenetic mineral、diagenesis types、intergrowth sequence、diagenetic facies、diagenetic phase of outcrop sandstones and core sandstones, provide academic precondition condition for studying better reservoir in studied region.

本次论文的研究目的是描述露头砂岩和岩芯砂岩岩石学特征并判断其岩石类型,比较露头砂岩与岩芯砂岩在自生矿物、成岩作用类型、共生序列、成岩相、成岩阶段以及成岩流体演化方面的异同,为研究区有利储层的研究提供理论前提条件。

In Chapter Four, combining with sequential semidefinite programming method and filter technique, we present a Filter-SSP method with a trust region-type globalization for the solution of nonlinear SDP problems.

在第四章中,结合序列半定化方法与过滤集技术我们给出了解非线性半定规划问题的带有信赖域策略的过滤集序列半定化方法。

Because of the lower density of the rejected solute during the horizontal solidification of NH4Cl-70%H2O, channels grow slantways and upwards and A type segregates form in the upper region of mush.

NH4Cl-70%H2O侧向凝固时,析出的溶质密度较小,偏析通道倾斜向上生长,在糊状区上部形成A偏析。

Following the tenet of "Taking techonology innovation as the lead, Several industries deveplopment as the direction, Entironment creating as the emphasis , Region economy prospering as the task ",Shandong Gaolu Aquatic Product Co.,Ltd,has developed to a techonology-type and high-standard modern enterprise ,with the spirit of "Solidary,Down-to-earth,Trustworthy,Looing fo r the truth" and the "Gaolu Brand " products has enjoyed a good reputation at home an d abroad.

山东高绿水产有限公司多年来以&科技创新发展为先导、多业并举生产为方向、创造生态环境为重点、振兴区域经济为已任&的宗旨,以&团结、务实、守信、求真&的企业精神,发展成为如今的科技型、高标准的现代化企业,所打造的&高绿&品牌的各种产品享誉海内外。这一切离不开社会各界人士对我公司的支持与厚爱。

Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明,T NF的两种形式在受体结合、生物学效应等方面各具特征[3,4]。M-TNF通过直接接触在组织局部中发挥效应的特性,有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

在克隆M-TNF cDNA的基础上,删除两型TNF转换酶酶解部位的编码序列,构建出mTNFm并测序验证;建立只表达膜型T N F的真核细胞株。

A kind of M-TNF recombin ant with a stable,uncleavable mutant typewas constructed for its gene therap y. Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明,T NF的两种形式在受体结合、生物学效应等方面各具特征[3,4]。M-TNF通过直接接触在组织局部中发挥效应的特性,有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

After detail field inspecting, combined with the deep indoor research result, the author regarded that those deposits, which districted in West Kunlun Mountain Region , are belong to Mississippi Valley Type Lead-Zinc deposits.

本文运用区域成矿学理论从沉积建造、岩浆活动、构造演化以及区域地球物理场和地球化学场特征等方面系统地阐述了西昆仑地区(特别是奥依塔格—库尔良晚古生代裂陷盆地)的成矿地质条件。

Type of oil-gas reservoirs were dissected, the result shows that S_(2+3) oil layer inwest slope region have four types oil-gas reservoirs, anticline, lithotogic updip pithing, structural-lithologic,fault barrier. Gas lies upper but oil lies bottom in the same layer, oil-gas distribute mainly in Taikang upliftzone and nearby in the plane, the types of oil-gas reservoirs are anticline and structural-lithologic inTaikang uplift zone.

通过典型油气藏解剖得到西斜坡区萨二、三油层油气藏主要有背斜、岩性上倾尖灭、构造—岩性、断层遮挡4种类型油气减,同一油层气在上油在下,油气在平面上主要分布在泰康隆起带及其附近,泰康隆起带以背斜和构造—岩性油气藏为主,西部超覆带以不整合和岩性上倾尖灭油气藏为主。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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