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The morphologic changes of these three tissues were observed with light microscope and transmission electronic microscope; the pulmonary wet-to-dry weight ratio, the pulmonary weight indexs, and the ratio of the area of the multilamellar body to the area of cytoplasm in type II alveolar cell were measured. Results 1.The permeability of the pulmonary endothelial cells increased 2 hours after the injection of oleic acid.

经光镜和透射电镜观察对照组和实验组三种组织的形态学改变;并测定肺组织的湿干重比和肺系数;采用立体计量法测定肺II型细胞板层小体与细胞质面密度比。

The type III cells were mainly located in the necrotic area or around the necrotic area.

III型细胞主要分布在坏死区或其周围。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周,炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节,另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg,20分钟后踝关节照光,激光波长627.sum,功率密度 100mwcm',照射时间1000秒,能量密度100)/。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径,治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后,治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗,随机治疗一侧膝关节,另一侧作自身对照。兔耳静脉注入I'arrainrelomg/Kg,20分钟后,膝关节用金蒸气激光照射,激光能量密度100)儿旷。24 /J'时后取膝关节滑膜作病理检查,并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果: 1。模型观察:CIA大鼠炎症高峰期滑膜下炎细胞浸润明显,滑膜细胞明显增殖,炎症达到高峰后二周,血管缀形成,并侵蚀和破坏软骨和骨, CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生,滑膜下炎细胞浸润,也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明,各组织对HMME 的吸收速度和吸收量不同,荧光值一时间曲线不同,滑膜组织比皮肤和软骨对 HMME的吸收多,在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明:PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性,但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好,统计学检验差异有显著性。。3_军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少,图像分析结果表明面密度(阳性染色的面积总和与统计视野面积的比值)治疗组小于对照组,统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多,图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组,统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小,与对照组相比,统计学检验差异有显著性。结论:二。CIA、AIA的病理改变类似人类RA,可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同,滑膜组织比皮。

We consider that there is geochemical anomaly pattern of typical porphyry copper deposit in the Qulong area,through systematic exploration of geochemistry in the mineral district with the geological and structural data. The degradation level of orebody is very shallow. The Qulong area is a good prospect to looking for supper large-scale copper-molybdenum deposit with the porphyritic type Cu-Mo mineralization as its main ingredient.

通过测区系统的地球化学勘查工作,结合地质构造资料,认为驱龙地区存在典型的斑岩铜矿床地球化学异常模式,矿化体剥蚀程度很浅,是找寻以斑岩型Cu-Mo矿化为主的超大型铜钼矿床的良好远景区。

According to the principles of synthesis, dominance, regionality and relative consistency, the environment degradation evaluation system is divided into three classes: vulnerable region, vulnerable type and vulnerable cell. The research area belongs to the erosion degraded Southern Red Hilly Area region. Based on the conceptions and grading, the dissertation probes into the methodology of evaluation and case study, and establishes a potential degradation evaluation system which comprises altitude, gradient, soil erosion index K, annual rainfall, the proportion of rainfall in raining period, and the drought index of rainless period.

在脆弱性概念及其分类的基础上,本文进行了脆弱性评估的方法探讨和实证研究,在脆弱性分析的基础上,选择高度、坡度、土壤可蚀性K值、降雨量、暴雨量、多雨期降雨比重和少雨期干旱指数为潜在脆弱度指标;选取作为区域人地关系、社会经济发展与管理水平综合影响结果的人类活动指标森林覆盖率和坡耕地指数作为现实脆弱度评估的人为加速侵蚀的修正指标,计算生态环境的现实脆弱度,并将其指标体系与方法用于衡阳盆地生态环境脆弱度评估的实证研究。

Methods Fifty healthy SD rats were fed for twentytwo months in order to build the model of aging rats, the histology of femoral bone, tibial bone and lumbal vertebra was used to observe, then we evaluated the indexes which reflect the degeneration of intervertebral disc including the quantity of collagen type Ⅱ,Ⅹ of intervertebral disc by the immunohistochemistry methods, the relative area of vascular bud, the thickness of calcified layer and uncalcified layer on cartilage endplate and the indexes which reflect the osteoporosis including the thickness ratio of cortical bone, the relative area of bone trabecula, the bone density, the max stress and straining of bone.

50只健康纯种SD大鼠,喂养22个月,建立老龄大鼠模型,进行股骨、胫骨、腰椎等组织学观察,并评价椎间盘退变的相关指标(椎间盘软骨Ⅱ型和Ⅹ型胶原表达、软骨终板血管芽相对面积、软骨终板钙化层/非钙化层厚度)与骨质疏松的相关指标(骨皮质厚度指数、骨小梁相对面积、骨密度、骨最大应力和应变)的相关性。

Results There was moderate or strong negative correlation among the indexes of the degeneration of intervertebral disc including the relative area of vascular bud, the ratio of the uncalcified and the calcified layer, the quantitation of collagen type Ⅱand the indexes of osteoporosis including the thickness ratio of cortical bone, the relative area of bone trabecula, the density of femoral bone and vertebral body bone, the max stress and straining of bone.

结果 骨质疏松相关指标骨皮质厚度指数、骨小梁相对面积、股骨密度、椎体骨密度与椎间盘退变指标终板血管芽相对面积、非钙化/钙化层比值、Ⅱ型胶原定量之间存在中度到强度的负相关,而与Ⅹ型胶原表达存在中度到强度的正相关,其中椎体骨密度与软骨终板的Ⅹ型胶原相关性最强。

In percentage of the tiller ear number/the tiller number, the increased ear number per unit area, the like grain number per ear and weight of 1, 000 grains; a bit shorter plants; almost the same base internode length, weight per unit internode length, internode length under ear, LAI values in middle and later stages, process of dry matter accumulation from straight growth onwards, dry matter accumulation amount from earing to maturity, length of flag leaf, 2〓 leaf, 3〓 leaf and 4〓 leaf from top and total area of these leaves, leaf thickness, mean canopy leaf tilt angle, light extinction coefficient , ratio of light penetration in the upper, middle and lower parts of the wheat canopy, and fair plant type; almost the same senescence process of flag leaf; and slightly increased root amount in 0-120cm soil layer and reduced root amount in 90-120cm soil layer; the optimized irrigation-optimized fertilization with crop residues improved the growth and development of the winter-wheat.

秸秆还田有利于改善冬小麦的生育状况。

Synthetic Aperture Radar is the more ideal remote sensing tool to survey the range of flood area;SPOT image data is applied to land use classification.It arranges two kinds data and computes the total area and the land use type of flood zone,and excepts to bring humanistic,social and economic data into the flood management system.It can help the operation and development of flood disaster prevention system.

合成孔径雷达为测绘洪水区域范围较为理想可行的遥测工具;而SPOT卫星影像资料则是应用于土地利用分类上,以GIS整合两种资料并计算出洪泛区域之总面积及其土地利用形态,期能将人文、社会及经济等资料纳入洪水管理体系之发展,有助于水利防灾整套系统之运作。

To investigate the characteristics of arsenic methylating metabolism in population in coal-burning type arsenism endemic area and in control area, and to explore the difference between the two population.

目的 描述燃煤型砷中毒病区人群与非病区人群砷甲基化代谢的特征,比较两区人群砷甲基化代谢水平的差异。

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