查询词典 type 1

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

In the course of establishing standard operating procedure, The temperature, time , the feasibility without decorticating and efficiency were investigated when alliinase was deactivated used a dry heating apparatus in tunnel type, but the efficiency can be achieved less than 70%, need to be improved; The reformative cribriform plate has been proved to be good in practice result in pulping enzyme-deactivated garlic without dehulling; The extraction efficiency achieved more than 90% when volumic ratio of 20% alcohol and water is 3 I 1 and 2:1 was adopted to extract alliin two times respectively; During exact filtration, the ratio of diatomite in precoating and filtration was 3% and 1% respectively, the losses of alliin adsorbed by diatomite in extraction solution were not remarkable. In the stage of alliin isolation by ion exchange resin, three simple and sensitive detected measures were successfully adopted to control the whole process; the efficiency of alliin isolation was similar to laboratory results; The alliin yield was over 50% in acidic fractions and the purity quotient of alliin achieved above 50% after drying; Furthermore, the phenomenon was found that losses of alliin were seriously affected by the temperature and time periods in concentration.

在制定中试生产标准操作规程中，考察了隧道式干热烘干机杀酶的温度、时间和效率，干热灭酶效率只能达到70%，待改进；改良的筛板使带皮打浆效果良好，证明非脱皮熟蒜打浆的可行性，减少了工序，降低了成本；提取过程中，20%酒精分别1∶3和1∶2提取2次，效率达90%以上；硅藻土板框过滤时，探索了精滤过程中提取液中预涂和过滤时硅藻土的比例分别为3%和1%，并考察硅藻土对蒜氨酸的吸附损失，滤液澄明度良好，蒜氨酸损失很少；在树脂吸附分离阶段，采用三种简便灵敏检测手段控制生产过程，分离蒜氨酸的效果与实验室一致，酸性流分中蒜氨酸收率可达50%以上，干燥后蒜氨酸纯度达50%以上；试验发现浓缩蒜氨酸洗脱液时温度和浓缩时间对蒜氨酸损失的具有很大影响。

The introduction combines traditional Chinese and Western medicine below the experience of enteritis of noxiousness of fine ailment of remedial Tibet mastiff, in order to offer reference. 1 hair sick condition and clinical expression ill dog are 2~4 month more age young dog, majority is come on suddenly, anorexia or abandon absolutely, mental depressed, systemic symptom worsens quickly, produce acuteness sex vomiting and diarrhoea, puke is first feed rotten, afterwards is yellow or olive bubble mucus and hematic type thing, have diarrhoea dung has the sticky stiff thing of grey yellow, turn after for rare dung of effluvial shape of embedded mucous membrane, then shows hematic dysentery, because ill dog is acuteness vomiting and diarrhoea are rapid dehydration, eyeball cave in, temperature is shown two-way and calorific,℃ of the 40~41 at the beginning of disease, 1~2d falls it is normal to come, after 3~4d answer elevatory, die very quickly next, 4~5d of course of diseases, the temperature when on one's deathbed drops more to normal temperature the following. Bottom of stomach of dog of die in one's bed of 2 analyse check has haemorrhage sex inflammation, show cardinal, small intestine mucous membrane falls off, alvine wall attenuates, there is gules mucus inside, mix inside large intestine have show wine blood excrement and urine, there are a lot of haemorrhage places on mucous membrane, haemorrhage of rectum mucous membrane is more, mesentery lymph node enlargement, sometimes afterwards sends intussusception, alvine tangent plane bleeds, cardiac muscle is loose, color becomes weak, cystic plentiful, liver is qualitative fragile and brittle. 3 diagnose 3.1 epidemiology to diagnose this ill much hair at young dog, rather dog of epidemic disease have an inoculation feels the most easily, be affected directly or affect secondhand, basically pass enteron infection, this disease all can happen all the year round, but with cold...

下面介绍中西医结合治疗藏獒细小病毒性肠炎的心得，以供参考。1发病情况和临床表现病犬多为2~4月龄幼犬，多数为忽然发病，食欲减退或废绝，精神沉郁，全身症状急剧恶化，发生剧烈性呕吐和腹泻，呕吐物先为食糜，继为黄色或黄绿色泡沫黏液和血样物，泻粪有灰黄色的黏稠物，后转为恶臭的内含黏膜状稀粪，继而呈血痢，由于病犬剧烈呕吐和腹泻迅速脱水，眼球下陷，体温呈双向发热，病初40~41℃，1~2d降至正常，3~4d后又复升高，然后很快死亡，病程4~5d，临死时体温多下降至常温以下。2剖检病死犬胃底部有出血性炎症，呈深红色，小肠黏膜脱落，肠壁变薄，内有红色黏液，大肠内混有呈暗红色血液粪便，黏膜上有许多出血点，直肠黏膜出血较多，肠系膜淋巴结肿大，有时继发肠套叠，肠切面出血，心肌松软，颜色变淡，胆囊充盈，肝质脆而易碎。3诊断3.1流行病学诊断本病多发于幼犬，未免疫接种犬最易感，直接感染或间接感染，主要通过消化道感染，该病一年四季均可发生，但以寒。。。

Type III is composed of latite and benmoreite, which are enriched in Al and Ti, with Na2O/K2O≥1.1 and no negative Eu anomaly (δEu=0.93–1.06), and Th/Ta and Ta/Hf ratios indicate a continental intraplate extensional environment. Its formation is probably related to underplating of basaltic magma.

第Ⅲ类由安粗岩，歪长粗面岩组成，富Ti，Al，Na2O/K2O≥1.1，Eu无异常(δEu=0.93～1.06)，Th/Ta，Ta/Hf值显示大陆板内伸展环境，其形成可能与玄武质岩浆的底侵作用有关。

The reaction of cyclic b-keto esters with CF3CO2ZnCH2I provides the corresponding ring-expanded products with the advantage of mild condition in moderate to good yields. A simple and efficient procedure has been developed for a variety of 1,3-diketones converted into the corresponding 1,4-diketones and trans-1,2-disubstituted cyclopropanols in high yields. In conclusion, this new type of organozinc reagents has been developed for many useful reaction in organic synthesis.

利用有机锌试剂所具有的双重特性，在新型的Baylis-Hillman反应中，可以直接与炔基酮和醛反应一锅制得多官能团的三取代的顺式烯；在b-酮酯化合物的扩环反应中，取得了重要进展，得到了增加一个碳的扩环产物，&一步法&合成，反应条件温和、环境友好；在1，3-二酮分子中成功地插入1个碳，合成出1，4-二酮和环丙醇，为现代有机合成提供了新的合成方法，具有良好的应用前景。

Methods 150 patients with prostatitic of Ⅲa type were randomly divided into two groups. Patients in treatment group (n=75) were treated by combination of extraorgan short wave therapy (once 20 mins/day), wild chrysanthemum suppos.(a piece, once a day, take by anus) and controlled release tablets of Doxazosini Mesylas (40 mg, once a day). Patinets in control group (n=75) were treated by extraorgan short wave therapy.

将150例Ⅲa型前列腺炎患者随机分为2组，治疗组75例予体外超短波（每次20 min，每日1次）联合野菊花栓（每次1枚，每晚1次肛门给药）及甲磺酸多沙唑嗪控释片（每次4 mg，每晚1次睡前服）治疗，对照组75例单纯予体外超短波治疗。2组均14日为1个疗程，1个疗程后统计临床疗效和治疗前后慢性前列腺炎症状评分。

Methods 150 patients with prostatitic of ⅲa type were randomly divided into two groups. patients in treatment group (n=75) were treated by combination of extraorgan short wave therapy (once 20 mins/day), wild chrysanthemum suppos.(a piece, once a day, take by anus) and controlled release tablets of doxazosini mesylas (40 mg, once a day).

将150例ⅲa型前列腺炎患者随机分为2组，治疗组75例予体外超短波（每次20 min，每日1次）联合野菊花栓（每次1枚，每晚1次肛门给药）及甲磺酸多沙唑嗪控释片（每次4 mg，每晚1次睡前服）治疗，对照组75例单纯予体外超短波治疗。2组均14日为1个疗程，1个疗程后统计临床疗效和治疗前后慢性前列腺炎症状评分。

The results indicate that the presence of GSH increased the expression of exoY、exoS、phzA1 and gacA,while no difference was observed with others virulence factor genes tested.PAO1 produced markedly less pyocyanin than the wild type strain PAOIP＜0.01Treating with GSH, the PCN production of PAOI(△gshB was increased from 0.47μg/mL to 1.73μg/mL (P＜0.01),the PCN production of PAO1 increased from 1.3μg/mL to 1.9μg/mL(P＜0.05),this result indicates that GSH upregulates PCN production.

结果发现，GSH对启动子库中exoS、exoY、phzA1和gacA等致病性因子的表达有促进作用，对其余测试过的致病性因子则没有明显影响；铜绿假单胞菌分泌的重要致病因子绿脓菌素(pyocyanin，PCN)的含量，在PAO1中远低于PAO1P＜0.01加入GSH后，PCN平均含量在PAO1(△gshB中从0.47μg/mL提高到1.73μg/mL，差异十分显著(P＜0.01)，在PAO1中从1.3μg/mL提高到1.9μg/mL，差异显著(P＜0.05)，表明GSH对铜绿假单胞菌生产PCN有促进作用。

We found, GLP-1-IR cells in various guts of rat, pig and foetus of human with distribution scatteredly among the basal portion of intestinal glands. The cell shape corresponded with open type cells, i.e. L-cells. The density of GLP-1-IR cells was different in races, but the distribution regularity of GLP-1-IR cells was same. It showed that a continuous increase from the proximal to the distal portion of small and bowel.

结果发现：在各段肠管都可见到GLP-1-IR细胞；GLP-1-IR细胞散在于肠腺的柱状上皮细胞之间，多位于肠腺的基底部，呈开放型内分泌细胞形态，即L细胞；不同种属问GLP-1-IR细胞密度不同，但分布规律相同，即自小肠和大肠的近端向远端逐渐增大。

The results showed that there were differences among treatments in transformation of high quality gene(Glu\|D 1 5+10) in F 1 grains, and heterozygous type was more frequently appeared in the treatment of irradiation. In the F 2 segregative population, heading date deviated to early\|mature parent in the treatment of reciperocal cross and its combination with irradiation, indicating that cytoplasm and irradiation could promote the heading. In F 3 the expressive frequency of (5+10) gene in the cross was lower than that in reciperocal cross, and irradiation treatment could raise the expressive frequency of(5+10) gene. These results showed varience in different crosses.

结果表明，不同组合处理间F1籽粒优质亚基(Glu D15+1 0 )的转化存在差异，辐射处理的多以杂合型出现。F2 分离群体中，反交及反交结合辐射处理的抽穗期明显倾向于早熟的亲本，细胞质与辐射具有促进抽穗期的作用。F3 植株Glu D15+1 0亚基的表达频率，不论是哪种组合，反交时 5+1 0亚基的表达频率高于正交；无论正交还是反交，辐射处理提高了 5+1 0亚基的表达频率，而且在不同组合间存在差异。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。