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In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene (COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

但微重力对相关基因调控的影响知之甚少，本研究以构建的基因工程细胞株为对象，以回转器模拟微重力效应，利用增强型绿色荧光蛋白（enhanced green fluorescence protein，EGFP）荧光半定量和细胞微丝荧光染色分析技术，探讨回转模拟微重力条件下，细胞微丝系统对I型胶原α1链基因（collagen type I alpha chain 1，COL1A1）启动子活性的影响。

In this study,we focus on the interaction between nuclear modifier gene MTO2 and aminoglycosides,and have received the following innovations.1,We here report first that the knockout of MTO2 significantly inhibited mtDNA p_(454)~R mutant's sensitivity to aminoglycoside antibiotics.Here are the results:(1)In neomycin GYP medium,when mitochondria genes were wild type,whether MTO2 knockout or not,the growth of yeast strains has no significant difference;when mitochondria genes were complete deletion,whether MTO2 knockout or not,the growth of yeast strains also has no significant difference;Interesting,when mtDNA P_(454)~R,wild-type MTO2 strains were hardly survival in neomycin GYP medium,but the growth of MTO2 knockout strains were only partly inhibited,there was a significant difference performance of neomycin sensitivity between them.

具体结果如下：(1)在含有新霉素的葡萄糖培养基上，mtDNA为野生型、MTO2敲除与否，相应的酵母菌株的生长没有显著差异；mtDNA完全缺失、MTO2敲除与否，相应的酵母菌株的生长也没有显著差异；然而，当mtDNA存在P_(454)~R突变时，MTO2野生型菌株几乎完全不能生长，而MTO2敲除菌株的生长只受到部分抑制，表现二者对新霉素的敏感性存在显著差异。

Slug:(1) A strip of metal, usually six points or thicker, for spacing lines of type,(2) A line of type cast in one solid piece on Linotype or Ludlow machines.

1铅条，(2)铅字条：(1)是6点或更厚的活字行隔金属长条。(2)在或排铸机上铸成的整行字条。

It includes four parts: 1 Part I, it introduces the basic knowledge of computer viruses such as computer virus definition, history and important viruses events, features, behavior phenomena, difference with computer software and hardware troubles, damage behavior and capability, classification, naming, future trend, etc; 2 Part II, it introduces the corresponding knowledge of computer systems related to computer viruses such as the composing of software and hardware of the computer system, storage media and its working mechanism, interrupt technologies,.com/.exe/.pe file formats and their working mechanism, etc; 3 Part III, it introduces computer virus mechanism and theory such as computer virus structure, work flow, working mechanism of key modules, typical technologies used to design computer viruses including the corresponding traditional technologies (interrupt filching, memory resident, etc.), the corresponding new routine technologies (self-encrypting, Mutation Engine, etc.), and the corresponding new technologies used by some prevalence computer viruses such as macro viruses, e-mail viruses, worm, hacker, Trojan, mobile phone viruses, the working mechanism analysis of some kinds of typical and prevalence computer viruses such as file-type viruses, macro viruses, e-mail viruses, worm viruses, hacker, Trojan, mobile phone viruses, the analysis of some typical and prevalence computer viruses examples including BALL viruses, WORD macro viruses, WantJob viruses, Code Red viruses, BO Trojan, etc; 4 Part IV, it introduces the defense and killing technologies of computer viruses such as the aim and criterion of computer viruses defense and killing, prevention methods and corresponding technologies, detection technologies (comparison method, character code scanning method, behavior inspecting method, analysis method, etc.), manual and automatic killing technologies, immunity technologies such as IBM digital immunity system, new anti-viruses technology trends (real-time anti-viruses technologies, 32 kernel technologies, active kernel technologies, etc.), some typical virus defense and killing softwares (Symantec AntiVirus product, PC-Cillin AntiVirus product, etc.), the defense and killing method analysis of some kinds of typical and prevalence computer viruses (file-type viruses, macro viruses, worm viruses, hacker, etc.), for example, firewall and intrusion detection technologies for anti-hacker, the defense and killing of some typical and prevalence computer viruses examples including WORD macro viruses, Code Red viruses, BO Trojan, etc.

课程内容具体包括四大部分：1）第一部分，介绍计算机病毒基本知识，包括：计算机病毒定义、病毒发展史及重大事件、病毒特点、病毒表现现象及与软硬件故障的区别、病毒破坏行为及危害性、病毒的传播途径及媒介、病毒分类、病毒的命名及计算机病毒技术发展趋势等内容；2）第二部分，介绍与计算机病毒有关的计算机系统相关知识，包括：计算机系统软硬件组成、存储介质结构及工作原理、计算机系统引导机理及流程、中断技术、。com/。exe/。pe等文件格式及工作机理等内容；3）第三部分，讲解计算机病毒机理，包括：计算机病毒的组成结构、病毒工作流程、病毒引导/触发/感染/破坏等模块的工作机理、计算机病毒所采取的编制技术（包括中断窃取/内存驻留等传统编制技术、自加密/隐形/变形机等新的常规编制技术、宏病毒/电子邮件病毒/网络蠕虫/特洛伊木马/黑客/手机病毒等一些新的流行病毒所采取的编制技术等）、一些类型的典型或流行计算机病毒的工作机理分析（包括：引导型病毒、文件型病毒、宏病毒、电子邮件病毒、蠕虫病毒、黑客、特洛伊木马、手机病毒等）、一些典型或流行的计算机病毒实例剖析（包括：小球病毒、WORD宏病毒、求职信病毒、红色代码病毒、冰河木马等）等内容；4）第四部分，讲解计算机病毒防治技术，包括：计算机病毒防治目的、病毒防治策略及规范、病毒在管理和技术上的预防措施、病毒检查技术（包括：比较法/病毒特征码扫描法/行为监测法/虚拟执行法/分析法等）、手工和自动病毒清杀技术、病毒免疫技术（包括：针对某种一次性感染病毒的基于病毒标签的免疫方法/基于自我完整性检查的计算机病毒免疫方法/IBM的数字免疫系统等）、反病毒技术的新发展（包括：实时反病毒技术/32位内核技术/主动内核技术/以毒攻毒技术等）、诺顿/趋势/金山等公司的病毒防治软件产品、一些类型的典型或流行的计算机病毒（包括：引导型病毒、文件型病毒、宏病毒、蠕虫病毒、电子邮件病毒、手机病毒、黑客、特洛伊木马）的防治措施（其中，也包括介绍面向防范黑客攻击的防火墙、入侵检测技术）、一些典型或流行的计算机病毒防治实例剖析（包括：WORD宏病毒、红色代码病毒、冰河木马v1.1/v2.2等）、多层次病毒防护体系等内容。

In the engine room there are two electrical compressors(type: CZ-5/30FZK). One air reservoir (type: A0.1-3CB*493-87)and control cabinet compose compress air system, the compressed air will be used for tyfon, sea chest and purging of CO2 piping, charge of pressure tank, miscellaneous air of engine room and pneumatic quick-close valves.

机舱设置电动空气压缩机组（型号：CZ-5/30FZK）2台，空气瓶（型号：A0.1-3CB*493-87）1只及控制箱等组成压缩空气系统，其压缩空气供气笛用气、海水箱和二氧化碳管系吹洗、压力水柜充气、机舱杂用及气动快关阀用气。

Fourteen cases were converted to laparotomy (including 4 cases of type I Mirizzi syndrome, 4 cases of type II which all converted to laparotomy. 2 cases with the location of cystic duct into the common bile duct much lower than normal one, 2 cases with the confluence of cystic duct and cho ledoch locating in the posterior wall of choledoch. 1 case with a public wall shared for about 2 cm between the cystic duct and the choledoch).

结果 全组90例，术中中转开腹14例（包括Mirizzi综合征Ⅰ型4例Ⅱ型4例全部中转；胆囊管汇入胆总管过低2例；胆囊管开口于胆总管后壁2例；胆囊管与胆总管共同一侧壁，并并行约2 cm 1例；右肝管汇入胆囊管1例，开腹术中证实）。

MMP-1 can denature and degrade fibrillar collagens, especially type I collagen and type III collagen in the certain site of them.

目前，对CHF患者血清MMP-1及TIMP-1水平变化与CHF功能状态、诊断、治疗与预后的关系的研究较少，国内还未见同类报道。

The main conclusions are the following:1 It is a effective means that making use of the high resolution remote sensing imagery has quickly and dynamically investigated and monitored the land use type ofRed Water River, and has carried through the four grades of classification, and its result has achieved the precision requirement of the minimum area of obvious land use type (cultivated land, field, economic forest) less than 9 pixel of imagery, area precision 9~27 pixel and 0.33 hectares.2 It is a high-efficient and feasible means that making use of the digital managing technology of wave band merging, precise geometry correction, imagery merging, imagery strengthening, imagery cut out and inlay, and making the orthograph, etc, and observing on the spot to data analysis, it has carried on people-computer interactive screen translating to the remote sensing imagery data of high resolution by using many kinds of geographical information systems soft ware.

主要结论如下： 1 利用高分辨率遥感影像进行快速、动态调查与监测红水河流域土地利用类型，并将土地利用类型进行到四级分类，达到重点地类图上最小面积9个像元，面积精度9～27个像元、1.5亩的精度要求，是一种行之有效的方法。

In this study, with a total of 798 SOC density data and the 1:500 000 soil maps, the SOC storage in Yunnan-Guizhou-Guangxi Region of China (including Yunnan province, Guizhou province and Guangxi Zhuang Autonomous Region) was estimated using the soil type method with the soil type method with the linkage of arithmetic mean method, median method, and area-weighted mean method (based on the area recorded in the Soil Series) based on different map units and the results were compared with that of the linkage of PKB method (Pedological Professional Knowledge Based Method).

利用中国滇黔桂地区（云南省、贵州省和广西壮族自治区）798个土壤剖面及1：50万土壤图在不同的制图单元水平上，分别采用算术平均值法、中值法和面积加权平均值法（以土种志中的面积为权重）估算了该地区1 m深度SOC储量，并与基于土壤学专业知识连接方法(Pedological Knowledge Based Method，PKB)的估算结果进行了比较。

The adjusted relative risk (95% confidence interval ) of MI associated with each standard deviation increase in anxiety variable was 1.37 (95% CI 1.12 to 1.68) for psychasthenia, 1.31 (95% CI 1.05 to 1.63) for social introversion, 1.36 (95% CI 1.10 to 1.68) for phobia, 1.42 (95% CI 1.14 to 1.76) for manifest anxiety, and 1.43 (95% CI 1.17 to 1.75) for overall anxiety. These relationships remained significant after further adjusting for health behaviors (drinking, smoking, and caloric intake), medications for hypertension, high cholesterol, and diabetes during follow-up and additional psychologic variables (depression, type A behavior, hostility, anger, and negative emotion).

心梗相关的焦虑变量升高的标准差校正的相对危险度为1.37(95%可信区间1.12到1.68)，这些联系在进一步校正健康习惯饮酒、抽烟和热量摄入）后，随访期间治疗高血压、高胆固醇、糖尿病和其他心理变量（抑郁、A型行为、敌意、愤怒和消极情绪）后变得更显著。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。