查询词典 two-times

Instead, the things you do at the end of your turn happen at two separate times.

取而代之的是你的回合结束时要做的事在两个独立时间发生。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

It is noteworthy that one, there was Jucheng Howe told Cuixi duty; Second, the need for back rent; Third, the two sides are deadlocked several times here, not calmly farewell.

值得注意的是，一、鞠成豪告诉崔喜那里有人值班；二、折回去。。。。。。

If you get two or three times down the court and miss, you can stay in a ballgame.

如果你在球场犯了两三次错误，你还是可以留在球场。

Indeed, Marquez defended the IBF bantamweight title seven times and won the world junior featherweight title in his first meeting with Vazquez. Vazquez made two defenses of the IBF super bantam title, won the world junior featherweight title, defended it twice and lost it to Marquez in their first fight.

事实上，在小马奎兹首战瓦兹奎兹并获得WBC次羽量级头衔之前，他已经7次成功卫冕了IBF雏量级头衔；瓦兹奎兹则是两次成功卫冕IBF超雏量级头衔，再获得WBC次羽量级头衔并成功卫冕两次，之后输给小马奎兹。

Hollywood made us medium-happy, too, with Guillermo Arriaga's The Burning Plain, a subtly epic tale of two women living different lives in different times, with Charlize Theron gnawed by slowly revealed guilts and griefs in present-day Oregon while Kim Basinger finds a tragic love-rapture in sunnier, earlier Mexico.

阿瑞加的《燃烧的平原》，也使我们比较开心。这是一个关于不同时代不同生活的两个女人的精巧叙事故事。查理兹？塞隆生活在今天的俄勒冈，被影片中慢慢揭示出来的罪恶感和痛苦所深深折磨，而金？贝辛格则在阳光更明媚的早年墨西哥陷入了一场悲剧性的爱情痴迷。

In addition this paper,"Bio-Rad method," two parasitic wasps of the post-embryonic development the process of protein accumulation in young bees and the host Plutella xylostella in this process in vivo haemolymph protein content were measured and found that over time, was gifuensis parasitic larvae of the diamondback moth haemolymph protein content showed an upward trend in nearly a straight line, Ichneumonidae parasitic For example, in the early parasitic diamondback moth haemolymph protein content also showed the trend of slow growth, but in the mid-and late parasitic young bee about to be completed in the development of the host body when to stop feeding, a significant downward trend却呈, and significantly lower than the third day of its own protein content; on the same day of the different species of the diamondback moth parasitic wasp hemolymph protein content compared Ichneumonidae Parasitism of diamondback moth have hemolymph protein content than in the third and fourth day after the parasitic Braconidae hemolymph content to be high, but very different in the case of the fifth day, as evidenced by her side after the parasitic bee moth haemolymph protein content is cocoon of the parasitic bee hemolymph protein content of 0.7 times.

另外本文采用"Bio-Rad法"对两种寄生蜂胚后发育过程中幼蜂蛋白积累及寄主小菜蛾在此过程中体内血淋巴蛋白含量进行了测定，结果发现随时间的推移，被茧蜂寄生小菜蛾幼虫的血淋巴蛋白含量是呈近直线增加趋势，而对于姬蜂寄生来说，在寄生初期小菜蛾血淋巴蛋白含量也呈缓慢增长趋势，但在寄生中后期，幼蜂即将完成在寄主体内的发育取食停止时，却呈大幅下降趋势，且明显低于其自身第三天的蛋白含量；对同一天的被不同种寄生蜂寄生的小菜蛾血淋巴蛋白含量进行比较，姬蜂寄生过的小菜蛾血淋巴蛋白含量在第三天和第四天均比茧蜂寄生后的血淋巴含量要高，但在第五天的情况大不相同，表现为被姬蜂寄生后的小菜蛾血淋巴蛋白含量是被茧蜂寄生的血淋巴蛋白含量的0.7倍。

You may have to repeat the removal process two or more times before the calluses are gone.

您可能不得不删除过程重复两次或两次以上的茧之前已经过去了。

This times Beira depth in the werewolf and the blood suck ghost two big camps among the most delicate and prettiest youth, is stranded for the sentiment, half sea water half flame is struggling own choice.

此时的贝拉深陷于狼人和吸血鬼两大阵营中最为俊美的少年中间，为情所困，一半海水一半火焰的挣扎着自己的选择。

I had begged her to help me learn to study with her three times that I should Xiezi "next to Xie" She then turned towards the One who is also embolden the paths, but according to the original up and down over the berm on both sides of the ladder on the dam pavilion li Also sitting two Laoke old man.

我只好求她帮我，跟她学念3遍我代蟹子"旁谢"她，然后壮胆走向那果然还在的小路，只是照原来多了护堤两边的上下阶梯，堤坝上的亭子里还坐着两位唠嗑的老人。

Apllied the data of QuickBird-2——the highest resolution remote sensing,and based on the theory and methodology of fractal geometry,we have quantitatively studied remote sensing lineations in Gaolong gold deposit of Guangxi.

采用Quick Brid-2高分辨率卫星遥感数据，通过分形几何学的盒计维数法，定量研究广西高龙金矿区遥感线性构造。

So, sometimes, to change the color can not be your food (referring to the above-mentioned crustacean) has been a sign of boiling over.

所以，有时，来改变颜色，不能你的食物一直是沸腾了的迹象。