查询词典 two-time

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

And they made two casings of gold, and two gold rings; and put the two rings in the two ends of the breastplate.

016 又作两个金槽和两个金环，安在胸牌的两头。

Measure the items on before and after the two courses of treatment respectively as follows:① Syndromes Investigation: to adopt the scoring methods of symptoms and signs with the certain standard scoring table of symptoms and classifying table of signs for the patients, on the day before and after the two courses of treatment respectively ,② Signs Investigation: the mucoma visibility, swelling or hyperemia conditions, or gloyp and polypoid change in the inferior and middle nasal conchas, nasal septum, etc with a nasal endoscope on the day before and after the two courses of treatment respectively,③ Test of the lung functiaon on the previous day before and after the two courses of treatment respectively.

治疗组在治疗前、两个疗程结束后分别进行以下项目的观察：①临床症状的评定：治疗前及两个疗程结束后当天对患者鼻塞、鼻痒、流涕、喷嚏症状的程度进行评分；②体征的评定：治疗前及两个疗程结束后当天采用鼻额镜肉眼观察患者下鼻甲鼻粘膜颜色、肿胀情况，及中鼻甲是否可见，有否息肉样变等；③治疗前及两个疗程结束后即刻予以检测肺功能。

Measure the items on before and after the two courses of treatment respectively (one course for 15 days) as follows:①Syndromes Investigation: to adopt the scoring methods of symptoms and signs with the certain standard scoring table of symptoms and classifying table of signs for the patients, on the day before and after the two courses of treatment respectively,②Signs Investigation: the mucoma visibility, swelling or hyperemia conditions, or gloyp and polypoid change in the inferior and middle nasal conchas, nasal septum, etc with a nasal endoscope on the day before and after the two courses of treatment respectively,③Test of the interferon-γ、interleukine-4(IL-4) in serum of patients by the method of enzyme linked immunosorbent assay on the previous day before and after the two courses of treatment respectively.

治疗组在治疗前、两个疗程结束后分别进行以下项目的观察：①临床症状的评定：治疗前及两个疗程结束后当天对患者鼻痒、鼻塞、流涕、喷嚏症状的程度进行评分；②体征的评定：治疗前及两个疗程结束后当天采用鼻额镜肉眼观察患者下鼻甲鼻粘膜颜色、肿胀情况，及中鼻甲是否可见，有否息肉样变等；③治疗前及两个疗程结束后一天清晨空腹抽取患者肘静脉血4ml离心后，采用酶联免疫吸附试验检测患者血清IFN-γ、IL-4的含量。

This aircraft uses control-figured vehicle design and nine independent control planes: two forward wings, two forward wing flaps, two inside elevons, two outside elevons, and one vertical rudder.

同时歼十使用了数字飞控设计，有9个控制翼面：两个在前翼，两个在前襟翼，两个在升降副翼中，两个在副翼之外，还有一个在垂尾上。

This aircraft uses control-figured vehicle design and nine independent control planes: two forward wings, two forward wing flaps, two inside elevons, two outside elevons, and one vertical rudder.

该机采用了control-figured vehicle design和九个单独机翼平面：2个前端机翼，2个前端小机翼，2个内部升降舵补翼，2个外部升降舵补翼和1个垂直舵。

Power Station where the main products are: the former pool water level monitor, oil mixing with water signal devices, oil mixing with water monitor, two two-FirstCall / 2 three / two four-way double-acting self-sustaining valve, speed monitoring devices, bearings oil level signal devices, flow, head efficiency monitoring device, the displacement angle of transmission controllers, intelligent two-way transfer of water supply valves, intelligent pressure transmission controllers, automatic self-cleaning water filters, small-caliber recoil-type filter, guided Leaf position switch, automatic qi device, float liquid level controller, temperature indicator, water level monitor, solenoid valve, electric butterfly valve, magnetic flap level gauge, linear displacement transducer, temperature data logging devices, shear sell-off signal devices, vibration, degree of monitoring devices placed, programmable speed monitoring devices, gate opening apparatus such as hundreds of professional instrumentation and automatic control device!........

其中电站主要产品有：前池水位监测仪、油混水信号器、油混水监测仪、二位二通/二位三通/二位四通双动自保持球阀、转速监测装置、轴承油位信号器、流量水头效率监测装置、位移角度变送控制器、智能双向供水转阀、智能压力变送控制器、全自动自清式滤水器、小口径反冲式滤水器、导叶位置开关、自动补气装置、浮球液位控制器、温度显示仪、水位监测仪、电磁阀、电动蝶阀、磁翻板液位计、直线位移变送器、温度巡检仪、剪断销信号装置、振动摆度监测装置、可编程转速监测装置、闸门开度仪等上百种专业仪表与自动控制装置！。。。。。。。。

The main production facilities consist of four seafood production workshops (total building area is about 6950 square meters and storage capacity is about 4000 tons )and three quick freezing room which made up aluminum plate (10 tons /each batch) and one ice-making workshop (its daly output of 35 tons )and one ice storage (total building area is about 350 square meters)and two fresh-freeing installation (the processing capacity is 1500kg/h)and one tunnel quick-freeing installation (the processing capaciyy is 600kg/h)and two production lines for processing shrimp and two production lines for cooking seafood and two production lines for processing fish and one flake ice maker(its daiy output of 35 tons)The plant built a laboratory for testing product quality,which had equipped with all equipments and instruments.

主要生产设施有：生产车间有4间，面积共有6950平方米；冷库5间面积共有3000平方米，储存量约4000吨；10吨/次铝板冷结间3间，原料保鲜库2间约250吨，日产35吨冰的制冰车间10间，储冰库350平方米。主要加工设备有：1500kg/h双螺旋单冻机2套；600kg/k隧道式单冻机1套；虾生产线2条；熟虾生产线2条；鱼生产线2条以及日产10吨片冰机1台。工厂建设有独立的产品质量检验室，配套完善的检验仪器设备，并拥有一批多年从事出口水产品加工和贸易，具有丰富实践经验的技术人员，能够适应现代水产品加工发展的需要。

Based on the presence of the two kinds of different basic modes that are being/have formed in the development of these two clan folksong-the keeping mode and the changing one, the dissertation intends to find out the origin of the generation of these two modes veiled behind its music form, to master the characteristics of different folk music deeply, and make some preliminary interpretation about the development of these two clan folksong.

在揭示两族民歌发展变化所形成的两种不同基本模式——保持型模式与变化型模式的基础上，力图发掘其音乐形态背后的内在成因，籍以深入地把握不同民族音乐的自身特征，并对两族民歌发展的过程作出初步阐释。

Xinjiang Hua Dianhong the wild goose pond power plant 220kV system altogether has four going beyond a line: Two Man Xian (2421), two lines (2422), two grablines (2423), two palace lines (2427), each line disposes WXB-11C and the WXB-15 double draught microcomputer protection.

新疆华电红雁池电厂220kV系统共有四条出线：二满线（2421）、二头线（2422）、二托线（2423）、二宫线（2427），每条线路均配置WXB-11C和WXB-15型双套微机保护。

Vishnu entered a dark fourth dimensional dream that did not support his field or continued life.

毗瑟挐进入了一个第四密度的黑暗梦想，那里并不支持他的能量场或继续生命。

Leaders and decision-making persons use it to collect the data, including the information of unit work, handing in fee, oweing fee, prepaying fee,changing and afterpaying and account transfering of joining-insurance employee, and account paying of all kinds of insurances from hospitalization insurance institutions.The collected data is picked up, organized, switched and showed to user.

该子系统主要面向各级领导、决策分析人员；从各个医疗保险经办机构和定点医疗机构采集数据，包括在各个医疗保险经办机构处理的单位办公信息，单位缴费、欠费、预缴费信息，参保职工变更信息，参保职工增减变动信息，参保职工补缴信息，参保职工帐户划拨信息：包括各定点医疗机构处理的各险种帐户支出信息，各险种的统筹金支付信息等；将采集的数据提取，组织和转换，然后展示给用户。