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Two molecular forms of Xenopus hatching enzyme, 60kD and 40kD molecules, was obtained during preparation and purification. Both of them were verified as the hatching enzyme molecules, using anti GST UVS.2 antibody as the probe. 60kD molecule was digested or autodigested easily into 40kD molecule during purification. It was indicated that 40kD molecule was probably derived from 60kD molecule with its two CUB repeats lost, and the two CUB repeats may play an important role in recognizing and/or modifying of the molecular structure of vitelline envelope.

在分离纯化非洲爪蟾孵化酶时,得到了60kD和40kD两种分子,用孵化酶的特异性抗GST-UVS.2抗体进行Western杂交的结果证明二者均为孵化酶分子。60kD分子很不稳定,在纯化过程中极易降解,40kD分子可能是由60kD分子经过降解或自身降解丢失了两个CUB重复区而形成的,而CUB重复区很可能在对受精膜分子结构的修饰或改造中具有重要作用。

One zippered horizontal welted left chest pocket; one zippered vertical welted right chest pocket; two handwarmer pockets set behind two patched-on flap front pockets; two interior security pockets.

左胸拉链横开口袋,右胸拉链直开拿破仑式口袋,两侧按扣搭片暖手袋,暖手袋背后隐藏两个横向口袋,两个内部拉链安全口袋,衣服拉链全为高等级YKK拉链。

In crystal, two APC-PY trimers associate into a hexamer by face. The assembly of two trimers within this hexamer is very similar to that of C-phycocyanin and R-phycoerythrin hexamers, but the tight level of the assembly of two trimers within this hexamer is clearly not so much as that of C-PC and R-PE hexamers.

晶体结构中,两个APC-PY的〓三聚体通过面对面的方式缔合成一个〓六聚体,该六聚体内两个三聚体的组装方式十分类似于C-藻兰和R-藻红六聚体,但六聚体内两个三聚体组装的紧密程度上显然不如C-藻兰和R-藻红六聚体。

The femtosecond irradiation spectra was used to measure the radiation kinetics process. Fitted by the two exponent model, two time peaks were obtained, corresponding to the interband radiation and the surface defect radiation respectively. This results also strongly suggests that there are two relaxation pathways in the nanocomposite.

利用飞秒发射光谱观测凝胶玻璃中ZnSe纳米粒子发光动力学过程,采用双指数模型进行拟合,得到了两个时间组分:11.2ps和550ps,分别对应纳米品的带间发光和表面缺陷态发光,进一步证实了半导体纳米晶材料受激辐射的两种发光途径的存在。

Two staff used to walk in the Light of hand, even the no umbrella; Two people sitting back to back and used on the lawn in the afternoon, even if the sun is dazzling; Two people used to the cold winter with a cup of coffee you hand me a mouthful, even if it already cold coffee through ......

习惯两个人手牵手漫步在绵绵的细雨中,哪怕没有打伞;习惯两个人背靠背坐在午后的草坪上,哪怕阳光很刺眼;习惯两个人在寒冷的冬天手握着一杯咖啡你一口我一口,哪怕这咖啡早已凉透。。。。。。

After fixing the two sides standard sections, the hoisting machine loosen rope, pull out the two end horizontal pin of pillar, lift the pillar to the place of two section standard section's middle angle steel, the operation staff leave the suspension coop.

待两侧标准节连接固定好后,卷扬机松绳,拔出顶梁两端横销,用手摇起重机上小吊杆将顶梁提到新接好的两节标准节中间框架角钢处,穿好横销并锁定,操作人员离开吊笼。

NEW YORK - There were two on, two outs and the home team down two in the last of the ninth, and Bobby Abreu watched strike three sail on by.

纽约讯-九局下半主场球队两出局两在垒时落后两分,阿不累唷眼睁睁看著第三个好球从眼前飘过。

Pharmacology function】Huang An the AN oxygen Mi Ding be in the Huang An medicine to suppress the germ a very strong kind of the function to synthesize anti-virus medicine, because of it with for germ to synthesize folic acid demand of to ammonia Ji the benzene be sour to have likeness on the structure, exchange with PABA to contend for two hydrogen folic acids to synthesize Mao, bar the germ synthesize two hydrogen folic acids to synthesize Mao, bar the germ synthesize two hydrogen folic acids, end influence nucleic acid protein of synthesize, repress thus germ of growth breed, to variety E-and E+very strongly repress a function.

磺胺间甲氧嘧啶是磺胺类药物中抑菌作用极强的一种合成抗菌药物,因其与细菌合成叶酸时所需要的对氨基苯酸在结构上具有相似性,与PABA兑争二氢叶酸合成酶,阻碍细菌合成二氢叶酸合成酶,阻碍细菌合成二氢叶酸,最终影响核酸蛋白质的合成,从而抑制细菌的生长繁殖,对多种E-及E+有很强的抑制作用。

METHODS:①Sixty samples were randomly divided into two groups(test and contrast groups,n=30 respectively),and the sciatic nerves of the toad was isolated.Two sorts of solution(alkalinized and plain bupivacaine hydrochloride) were applied to a segent of nerve,and the amplitude of the action potential was measured.②The grouping method was the same as above.After destroying the cord above the third spinal vertebrae,two sorts of bupivacaine soultion in various concentration were injected into abdominal cavity of the toad.The leg′s flexing time was measured.

①离体坐骨神经法:取蟾蜍60只,制备坐骨神经标本,分两组,实验组和对照组各30只,测定不同浓度的两组布吡卡因对离体坐骨神经动作电位幅度的影响;②在体坐骨神经丛法:取蟾蜍60只,分组同上,破坏第三脊椎以上的脊髓后,将不同浓度的两种布吡卡因注入蟾蜍的腹腔内,测定其缩腿反射消失的时间。

The ten patients of the avulsed fragment was reduced and fixed with two wire under arthroscopy.Result All patients were followed up from two months to two years.

方法对 10例(11膝)髁间嵴撕脱性骨折病人,在关节镜监视下进行骨折复位,通过两根钢丝套环引出固定钢丝固定骨折。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。