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There are three copies of R fragment in the S mt-genome, and each copy contains two closely adjacent open reading frames: orf355 and orf77. Among these three copies two BamHI fragments were cloned, and the sequence analysis showed that cox1 and cox2 gene lies upstream of the R region in each fragment, respectively. However, they locate in different strands and head to head with R. In this study, we found that:(1) The expression of cox1 and cox2 is not affected by the transcription of R region;(2) The two copies of R region transcribe in large amount in the microspores, and produce transcripts of 1.6kb and 2.8kb, respectively, in the sterile microspores; However, the abundance of these two transcripts is reduced in the fertility restored microspores, realized through the mRNA decay, and this reduction occurs at the uninucleate microspore stage;(3) In the sterile microspores, the 5 terminus of the 1.6kb transcript contains a palindrome region that can fold into a stem-loop.

本研究发现：(1) cox1和cox2的转录没有受到R区转录的影响；(2)两个拷贝的R区在在不育花粉中高丰度表达，其转录本大小分别为1.6kb和2.8kb；而在育性恢复的花粉中，这两个转录本的丰度被大大降低，进一步分析表明这一结果是通过mRNA分子的降解途径来实现的，此降解过程发生在单核花粉期；(3)在不育花粉中，1.6kb转录本的5′末端具有一段可形成茎环结构的迴文对称序列，而在可育花粉中，其5′末端短缺了9个碱基；(4)在不育花粉和育性恢复的花粉中，R区转录本的加尾位点都集中位于一个3′茎环结构之后；(5)不管是在不育花粉还是育性恢复的花粉中，orf77区域内均在第52位和100位核苷酸处发生了不同频率的C向U的编辑，从而形成UGA终止密码；(6)不管是orf55-orf77的成熟转录本还是转录前体分子，都已被加上了Poly尾巴，表明已进入降解途径；在以上实验结果的基础上，本研究对S型CMS花粉育性的恢复机理进行了如下推测：在不育花粉和育性恢复的花粉中，orf77区域内均发生了终止编辑，即由RNA编辑产生了提前终止密码子。

The same result can also be achieved when optimizing F8 function (Rastrigin's function). Comparing to EGA, KEGA is easy to realize and the increase of computation load can be neglected, but the performance of KEGA can be improved greatly. 2. To enhance the speed of AIA, two new methods of computing the concentration of antibody were developed. Simulation results for F8 and F15 (needle in haystack: type I) functions show that the two methods can improve the running speed of AIA more than 8. 5 times. 3. As to the drawback in defining antibody concentration, a new definition based on Euclidean distance between two antibodies and fitness is proposed. On this basis, a new AIA was constructed. The convergence performance are more better than that of AIA. 4. Combining king crossover with AAIA and DBAIA, two new AIAs, KAAIA and DKBAIA, were proposed.

仿真实验结果表明KEGA实现简单，与EGA相比，其增加的计算开销可以忽略，但性能改善十分明显； 2、针对基于信息熵的免疫算法运行速度慢的缺点，提出了二种能够加快AIA运行速度的新的抗体浓度计算方法，得到两种新的免疫算法，即加速的人工免疫算法(Accelerated Artificial Immune Algorithm，AAIA)和加速的人工免疫算法-2（Accelerated Artificial Immune Algorithm-2，AAIA-2），对测试函数F15(needle in haystack：type I)、F8函数的仿真研究表明，这两种算法的运行速度都是AIA的8.5倍以上； 3、针对AIA的浓度定义存在的缺陷，提出了一种新的基于抗体间欧氏距离和适应度的新的抗体浓度定义，并依据该定义构造了一种基于欧氏距离的人工免疫算法(Euclidean Distance-based Artificial Immune Algorithm，DBAIA)。

The two mesoscale vortices for east-west distribution are similar to azimuthally wave number two and intensify TC more strong than other two vortices distributing and weaker than actual azimuthally wave number two waves disturbance.

台风内核区和眼壁附近扰动的减弱使台风最大切向风速的增幅减小，且强度增强，风速变化的震荡性加强，震荡的周期缩短；强度减弱，风速变化的震荡性减弱，震荡的周期加长。

The phase diagrams of PEG/phosphate aqueous two-phase systems and the partition properties of BSA in the systems The PEG/phosphate aqueous two-phase system was regarded as the research object, the contents of phosphate and the polyethylene glycol in the system were measured by weighting and freeze-drying method respectively. Two diagrams were successfully drawn, and the characteristics such as binodal curves, tie lines and lever rule of the phase diagrams of the aqueous two-phase systems were described, as well as the achieved phase diagrams were qualitative analyzed with thermodynamics.

本论文以聚乙二醇／磷酸盐双水相系统相图和相分离过程及牛血清清蛋白在双水相系统中的分配特性为研究基础，从探讨超声波对双水相系统组成物质磷酸盐和PEG溶液的作用机理入手，建立了静态环境中双水相萃取过程的相分离数学模型，揭示了超声波对双水相系统组成和溶质分配效率影响的规律。

In the paper,the transmission of designed roller oscillating tooth reduction gear adopts osci llating tooth gear drive without exception,one-stage reduction gear and two-stage reduction gear have joined through spline coupling band;The joining way of shock wave thellomble and oscillating tooth adopts the biserial bearing of without inner ring of needle bearing,and directly adopts bearing outer ring as shock wave thellomble;Center wheel of two-stage reduction gear and center wheel of two-stage reduction gear passes through taper thread,and center wheel is fixed;As soon as the flange of input end and center wheel of one-stage reduction gear,the flange of export end and center wheel of two-stage reduction gear makes monobloc structure.

本文预设的井下减速器的传动均采用活齿传动，一级减速器的输出轴和二级减速器的输入轴通过花键联轴套联接；激波环与活齿的毗连方式采用能蒙受较大载荷的无内圈双列滚针不锈钢管，且直接采用不锈钢管外圈作为激波环；一级减速器的中心轮和二级减速器的中心轮是通过锥螺纹毗连的，且中心轮固定；一级减速器的中心轮与输入端的法兰盘，二级减速器的中心轮与输出端的法兰盘均做成整体的结构。

In the paper,the transmission of designed roller oscillating tooth reduction gear adopts oscillating tooth gear drive without exception,one-stage reduction gear and two-stage reduction gear have joined through spline coupling band;The joining way of shock wave thimble and oscillating tooth adopts the biserial bearing of without inner ring of needle bearing,and directly adopts bearing outer ring as shock wave thimble;Center wheel of two-stage reduction gear and center wheel of two-stage reduction gear passes through taper thread,and center wheel is fixed;As soon as the flange of input end and center wheel of one-stage reduction gear,the flange of export end and center wheel of two-stage reduction gear makes monobloc structure.

本文设计的井下减速器的传动均采用活齿传动，一级减速器的输出轴和二级减速器的输入轴通过花键联轴套联接；激波环与活齿的连接方式采用能承受较大载荷的无内圈双列滚针中国财经圈，中国财经圈，且直接采用进口中国财经圈外圈作为激波环；一级减速器的中心轮和二级减速器的中心轮是通过锥螺纹连接的，且中心轮固定；一级减速器的中心轮与输入端的法兰盘，二级减速器的中心轮与输出端的法兰盘均做成整体的结构。

The best survuval rate of each stage thawed-embryos existed in groups of Zygote EDT3O-two-step method with I mm equilibration(66%), of Morulae EDT3O-two-step method with I mm equilibration(94%) and EDT4O-two--step method with 0.5 mm equilibration(94%), of Blastocyte EDT3O-two-step method with I mm equilibration(91%) and EDT4O-one-step method with I mm equilibration(91%).

总之，我们的研究表明，DET玻璃液冷冻方法是简单、经济而又高效的，冷冻小鼠各阶段胚胎都能获得较高的存活率，其中受精卵的EDT30二步法1分钟平衡组(66％)、桑椹胚的EDT30二步法1分钟平衡组(94％)和EDT40二步法0.5分钟平衡组(94％)、囊胚的EDT30二步法1分钟平衡组(91％)和EDT40一步法1分钟平衡组(91％)冷冻效果最好。

Two steering cantalevers are fastened to the frame of vehicle. The two sections of the adjustable pole are reversely screwed. Pole and two sections are jointed by fastener and bolt, bolt of two sections need be loosened in order to circumrotate body of pole for adjusting length.

两个悬臂座分别固定在车架上，前后可调连杆两头为正反丝扣套接，平时紧固卡子和紧固螺栓将可调连杆杆身和两头的接头紧为一体，需要时松开两头紧固螺栓，旋转可调连杆杆身可达到伸缩调整。

The results show:(1) that the dynamical properties in the system depend on the degree of entanglement of the initial two identical two-level entangled atoms and the intensity of the coherent optical field, and the atomic dipole moment can be completely squeezed via choosing optimal degree of entanglement of the initial atoms in the feeblish optical field;(2) that the quantum properties of the system also depend on the degree of entanglement of the initial two identical two-level entangled atoms and the intensity of the coherent optical field, and the radiation field can be effectively squeezed via choosing optimal degree of entanglement of the initial atoms in the feeblish optical field.

结果表明：(1)相干态光场的强度和两原子的初始纠缠度对原子的动力学行为有很大的影响。原子布居的振荡频率及幅度和原子偶极压缩的演化规律与光场强弱和两原子初始纠缠度相关联。在弱光场下，选择合适的两原子初始纠缠态，原子偶极矩可以完全被压缩。(2)相干态光场的强度和两原子的初始纠缠度对光场的量子特性也有很大的影响。光场的二阶相干性质和光场的压缩效应均与光场强度和两原子纠缠度相关联。在弱光场中，选择合适的两原子初始纠缠度，光场压缩深度和时间可达到最大。

The invention provides a dual redundant CAN bus controller, which is arranged on a crewel CAN bus, characterized in that: two CAN bus transceiving modules, two initializing modules are respectively corresponding to the CAN bus controller module A and CAN bus controller module B; the two CAN bus controller modules are respectively connected to the two CAN bus via a driver shifting control unit; the initializing module is connected to the bus control initializing register for initializing the e CAN bus controller modules; a protocol processing module is connected to the FIFO buffer; the FIFO buffer is connected to the node processor via the logical interface; a intermitting process unit sends the corresponding intermitting control signal to the CAN bus transceiving modules according to the CAN bus controller modules; one path of the CAN bus controller module is directly connected to the protocol processing module; another path is connected to the protocol processing module via the bus receiving buffer.

本发明提供了一种双冗余CAN总线控制器，设置于双线CAN总线上，其特征在于：两个CAN总线收发模块、两个初始化模块分别与CAN总线控制器模块A和CAN总线控制器模块B相对应；两个CAN总线控制器模块通过驱动器切换控制单元分别与两条CAN总线相连；初始化模块与总线控制器初始化寄存器相连，对所对应的CAN总线控制器模块进行初始化；协议处理模块与FIFO缓存器相连；FIFO缓存器通过逻辑接口与节点处理器连接；中断处理单元根据CAN总线控制器模块的控制命令对CAN总线收发模块发出相应的中断控制信号；CAN总线收发模块一路与协议处理模块直接相连，另一路通过总线接收缓存器与协议处理模块相连。

Looking to bounce back from defeat against Cesena last weekend, the Calabrian boss will be sticking to his tried and tested 4-3-3, with Lopez and Sedivic joined up front in Giampaolo's absence by either Nanni or Plasmati.

鉴于上周末对切塞纳的比赛中反败为胜，这支南方球队将会更坚持其一贯而有效的4-3-3阵型，洛佩斯和塞德维奇将取代受伤缺席的詹保罗，和纳尼或者帕斯马蒂中的一个搭档锋线。

Meanwhile, their exposure to the more freewheeling ways of the West broadened dramatically.

另外，西方较自由的生活方式对年轻人的影响增大。