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Arturo Rollo managed it. I told our story in Two Types magazine, which is no longer published, and on my website .

Arturo Rollo 管理,我的故事,在 Two Types 杂志上发表,该杂志已停止发行,还有我的网页。

The 5'coding region of its eDNA was optimized according to the codon bias of Pichia pastoris to make it secretively express in Pichia pastoris with higher performance. Two-step DNA synthsis was used to synthesize the eDNA sequence being optimized of ADTZ. OPT-ADTZ was connected with constitutive plasmid pGAPZaA to construct the reecombinant plasmid pNOA. pNOA was linearized and then transformed into Pichia pastoris GS115. Then codonoptimized ADTZ was constitutively and secretively expressed in Pichia pastoris.

为了使重组蛋白rADTZ能在毕氏酵母中高效分泌表达,根据毕氏酵母密码子的偏好性对rADTZ的5'末端的编码区域进行了优化,利用two-stepDNAsynthsis技术合成出ADTZ优化的基因序列OPT-ADTZ,并与组成型表达载体pGAPZaA连接,构建重组质粒pNOA,线性化pNOA后转化至毕氏酵母GS115中,实现了密码子优化的rADTZ组成型分泌表达。

Styling / Convenience Engine Trim Black powder-coated with Wrinkle-Black treatment Exhaust System Chrome, staggered shorty duals Foot Supports and Controls Mid-mounted rider footpegs with brake and shift levers; standard passenger footpegs Handlebars Stainless steel, wide, dirt-track inspired Seat One-piece, two-up Sportster classic Instruments Electronic speedometer with odometer, time-of-day clock on odometer, dual resettable tripmeter, fuel gauge with low fuel warning light, low oil pressure indicator light, diagnostics readout, LED lights, tachometer Hazard Warning Integrated into turn signal controls Smart Security System Optional, next-generation hands-free security fob; operates based on proximity to vehicle

称呼/便利引擎修剪黑色粉末上漆与皱痕黑治疗排气系统镀铬物,摇摆的shorty 双倍脚支持和控制中间登上的车手footpegs 与闸和转移杠杆;标准乘客footpegs 把手不锈钢,宽,土轨道启发了位子单件, two-up Sportster 经典仪器电子车速表与测路器, time-of-day 时钟在测路器,双重resettable tripmeter,燃料测量仪以低燃料警告灯,低油压显示灯,诊断读出, LED 点燃,车头表危险警告集成轮信号控制聪明的保安系统任意,下一代没有雇工的安全fob;操作根据与车的接近度

Shift levers; standard passenger footpegsHandlebars Stainless steel, wide, dirt-track inspiredSeat One-piece, two-up Sportster classicInstruments Electronic speedometer with odometer,time-of-day clock on odometer, dualresettable tripmeter, fuel gauge withlow fuel warning light, low oil pressureindicator light, diagnostics readout,LED lights, tachometerSmart Security System Optional, next-generation hands-freesecurity fob; operates based on proximity

称呼/便利引擎修剪黑色粉末上漆与皱痕黑治疗排气系统镀铬物,摇摆的shorty 双倍脚支持和控制中间登上的车手footpegs 与闸和转移杠杆;标准乘客footpegs 把手不锈钢,宽,土轨道启发了位子单件, two-up Sportster 经典仪器电子车速表与测路器, time-of-day 时钟在测路器,双重resettable tripmeter,燃料测量仪以低燃料警告灯,低油压显示灯,诊断读出, LED 点燃,车头表危险警告集成轮信号控制聪明的保安系统任意,下一代没有雇工的安全fob;操作根据与车的接近度

The wampum, or beaded belt, showed two parallel lines on a pale background.

酋长们说,与其遵守《权利和自由宪章》,倒不如尊崇确定与非土著人关系的条约《the Two-Row Wampum Treaty》(易洛魁联盟与荷兰政府代表于17世纪签订,The wampum也叫 beaded belt,表示在灰白背景下的两条平行线)。

The wampum, or beaded belt, showed two parallellines on a pale background.

酋长们说,与其遵守《权利和自由宪章》,倒不如尊崇确定与非土著人关系的条约《the Two-Row Wampum Treaty》(易洛魁联盟与荷兰**代表于17世纪签订,Thewampum也叫 beadedbelt,表示在灰白背景下的两条平行线)。

Yet the "Desiderata" which Shore found among his papers may indicate that Jones had planned to spend additional time on linguistics: the third desideratum is "A Grammar of the Sanscrit Language, from Panini, etc."; the fourth,"A Dictionary of the Sanscrit Language, from thirty-two original Vocabularies and Niructi."

然而绍尔在他的文件中找到的 Desiderata或许暗示出琼斯本来计划在语言学上花费更多的时间:其中的第三项迫切需要获得之物是,&梵语语法,帕尼尼等&;第四项是,&梵语词典, from thirty-two Vocabularies and Niructi &。

As its title suggests, Two Fisted is a tough film.

就像标题让人联想到的一样,Two Fisted 是一部硬朗的影片。

To evaluate which combinations of preprocessing and differential expression method perform well, we consider 4 popular preprocessing methods (MAS 5.0, RMA ,dChip and PDNN) and 5 popular differential expression methods (fold-change, two sample t-test, SAM, EBarrays and limma).

为了评估各种预处理方法与表现量差异方法组合的表现,我们考虑了四种常用的预处理方法:MAS 5.0、RMA、dChip及PDNN,与五种常用的表现量差异方法:fold-change、two sample t-test、SAM、EBarrays及limma。

Rebuilt melters come with a one-year standard warranty and a two-year/4000 hour warranty on SP pumps and controls.?

重建melters配备了为期一年的标准保修和保修two-year/4000小时对SP水泵和控制。?

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

Several theories about reigniting and extinguishing of the arc have been refered.

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