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The relationships of VEGF with the indices of renal damage, including renal/body weight, urinary protein excretion, glomerular volume and glomerular area, were analyzed. Results The expression of VEGF mRNA in diabetic kidney was significantly up-regulated after operation from 2 weeks to 24 weeks with the peak level at 20weeks, when compared with control at the same time- points. The positive results of VEGF staining in diabetic glomeruli was increasingly observed after operation from 2 weeks to 24 weeks, with the peak at 20 weeks. The positive results of VEGF staining in diabetic tubuli was increasingly seen from 2 weeks to 24 weeks, with the peak at 8 weeks.

结果 VEGF mRNA在糖尿病模型组大鼠肾脏中持续高表达，各时点与对照组相比差异均有统计学意义(P.05)；糖尿病组2~24周肾小球VEGF蛋白表达强于对照组(P.05)，24周时有所减弱，但仍强于对照组；糖尿病组各时点肾小管VEGF蛋白表达均强于对照组(p.05)， 8周后表达明显增强；VEGF表达变化与肾质量／体质量(r=0.518， P＝0.001)、尿蛋白(r=0.409， p=0.001)、肾小球面积(r=0.499， P-0.000)和体积(r=0.499， P-0.000)呈正相关。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

1St Week: A general introduction to the nature and contents of the course; an investigation of students' acquired knowledge of literature in general; a brief introduction to African-American literature 2nd and 3rd Weeks: Rediscovering an Invisible Culture (Why Black culture had been unknown and unobserved for several decades; how it came to be rediscovered ) 4th to 6th Weeks: Double Consciousness (the marginal perspective in Language, Oral Culture, Folklore and Religion); Reading representative works by three Black writers of racial consciousness: Douglass, Washington and Bu Bois 7th to 9th Weeks: Minstrelsy (Imitation, Parody and Travesty in Black-White interaction rituals 1830--1920); Reading the novel about the Black life: Uncle Tom's Cabin 10th to 12th Weeks: Social Mobility and Cultural Stigma: The case of Chicago Jazz 1920--1930; Reading poems by Hughes and novels by Wright 13th to 15th Weeks: Oral Tradition and the Quest for Literacy: The crisis of Black writers from Philips Wheatley to Ralph Ellison; Reading the masterpiece of Ellison 16th to 18th Weeks: Contemporary Afro-American Culture: the sixties and seventies (efforts on reconstructing the Black identiy and Black history); Reading novels by Morrison

第1周：介绍课程性质、内容，了解学生相关文学背景，简要概括黑人文学历史第2-3周：再现黑人文化（黑人文化长期被掩盖的事实和原因，重新被发现的原因和过程）第4-6周：双重意识（语言、吟唱文学、民俗民谣、宗教的边缘化），阅读黑人种族意识三代伟大启蒙家道格拉斯、华盛顿、杜波伊斯的代表作第7-9周：吟游文学（黑人与白人的文化互动过程中的模仿、戏仿、曲仿），阅读第一部反映黑人生活的小说《汤姆叔叔的小屋》第10-12周：社会动荡与文化耻辱（芝加哥爵士乐的兴起、流行和影响），阅读休斯和赖特的代表作第13-15周：口头文学传统与争取教育权利的斗争（从惠特利到埃里森的黑人作家危机），阅读拉尔夫·埃里森的代表作第16-18周：六、七十年代以来的美国黑人文化（重建身份和重建历史的努力），阅读莫里森的代表作每位参加该课程学习的学生均须严格出勤，按时完成学读任务，每学期主持两次小型（10分钟）学术报告，第一次针对老师推荐的作家作品做一个专题报告，第二次针对自己的学期研究计划做一个选题前瞻性和可行性报告。

Part two Part two Okay lets do this here again in front of all of your friends I need a little room but dont go Cant be too far youre the star of my show And you dont need a mike to move the crowd Dont need a spotlight you smile, They wanna see the love we sold out I bet when its over, they go wow They know what it is, what it is Hard to end up with a couple of kids When they say, and the winner is They gonna be calling our card for the love again Give them what they want Show them how to love They want something new They want love part two They see action and lights They want that in their life They want love like us coz its new They want the love part two, part two The winners you this year,again A round of applause from all of them They try to memorise your lines I know you by heart, coz youre mine You dont need a crowd to do the show Dont even need the script coz you know That even when the lights go out The show must go on So I Give them what they want Show them how to love They want something new They want love part two They see action and lights They want that in their life They want love like us coz its new They want the love part two Its like a replay love Cut off that he she say love Turn up that make me stay love Its all that i want Somebody show your love Somebody out there want your love They stay through all of part one I know what they want, what they want Give them what they want Show them how to love They want something new They want love part two They see action and lights They want that in their life They want love like us coz its new They want the love part two What they want Show them how to love They want something new They want love part two They see action and lights They want that in their life They want love like us coz its new They want the love part two They want the love part two They want the love part two Baby,They want the love part two They want the love part two Love part two They want the love part two They want the love part two

第二部分第二部分这样好让这里的所有朋友面前再次我需要一个小房间但是dont去也不容过于youre我的一颗明星和你不需要一麦克风移动人群 dont需要聚光灯你微笑，他们希望看到我们的爱售罄我敢打赌，当它结束了，他们走哇他们知道它是什么，它是什么很难最终形成了两个孩子当他们说，获奖者是他们gonna是要求我们的爱再次卡给他们他们想要的东西告诉他们如何爱他们希望新的东西他们希望爱第二部分他们看到行动和灯光他们希望在他们的生活他们希望像我们这样的堂妹爱新他们希望爱第二部分，第二部分优胜者你今年再次来自所有这些全面的掌声他们试图记住您的线路我知道你的心，我的堂妹youre 你不需要人群做节目 Dont甚至需要脚本堂妹你知道这甚至当灯熄灭该比赛必须继续进行因此，我给他们他们想要的东西告诉他们如何爱他们希望新的东西他们希望爱第二部分他们看到行动和灯光他们希望在他们的生活他们希望像我们这样的堂妹爱新他们希望爱第二部分它像一个重放的爱切断她说，他爱打开了，使我留下的爱它的一切，我希望有人展示你的爱有人在那里要你的爱他们通过各种逗留第一部分我知道他们想要什么，他们想要的东西给他们他们想要的东西告诉他们如何爱他们希望新的东西他们希望爱第二部分他们看到行动和灯光他们希望在他们的生活他们希望像我们这样的堂妹爱新他们希望爱第二部分他们想要的东西告诉他们如何爱他们希望新的东西他们希望爱第二部分他们看到行动和灯光他们希望在他们的生活他们希望像我们这样的堂妹爱新他们希望爱第二部分他们希望爱第二部分他们希望爱第二部分婴儿，他们希望爱第二部分他们希望爱第二部分爱第二部分他们希望爱第二部分他们希望爱第二部分

Seventy-seven male big rats of four to five months old with the weight of two hundred and eighty to three hundred and twenty grams were divided randomly into contrast group of zero week,contrast group of three weeks,contrast group of seven weeks,contrast group of eleven weeks,experimental group of three weeks,experimental group of seven weeks and experimental group of eleven w...

选用280～320g 4～5月龄wistar雄性大鼠44只，随机分为0周空白对照组11只，3周实验组11只，7周实验组11只，11周实验组11只，0周空白组于0周处死，解剖取大鼠股骨，以生理盐水浸湿的纱布包裹，置-20℃冰箱内保存备用。

To survive since the age of about 1400 (or 130) years ago to 350 (or 30) years ago, the early representatives Ramapithecus (1400 million years ago to 700 million years ago), late representative is Australopithecus (50 million years ago to 100 million years ago).

生存年代约自1400（或130）万年前至350（或30）万年前，早期代表是腊玛古猿（1400万年前至700万年前），晚期代表是南方古猿（50万年前至100万年前）。

RESULTS AND CONCLUSION: PCHC: Intra-bone mechanical strength was lower at 2 weeks, the lowest at 4 weeks, but then closed to intension of normal cancellated bone at 8 weeks, higher than normal cancellated bone at 12 weeks, and recovered to the level of normal cancellated bone at 16 weeks.

结果与结论：多孔碳酸化羟基磷灰石骨水泥：2周时的骨内力学强度较低，4周时降到最低，8周时接近正常松质骨强度，12周时超过正常松质骨强度，16周时恢复到正常松质骨水平。

Microscope examination:in group A,at 2 weeks,a few inflamatory cells appeared in the holes with many osteoblasts and ostoid appeared around the drilled holes.At 8 weeks,marrow formed in the drilled holes in group B,at 2 weeks,there were a large amount of osteoblasts in the drilled holes and some ostoid formed around thc drilled holes.At 4 weeks,the drilled holes were full of trabeculae.At 8 weeks,the trabeculae matured with the marrow appeared in the intertrabecular space.

组织学结果：2周时A组钻孔区出现少许炎症细胞，边缘出现较多成骨细胞并有骨组织形成，至8周时，钻孔区内形成骨髓组织，只在边缘形成骨小梁结构。2周时B组钻孔区有大量的成骨细胞，边缘有较多骨组织形成，4周时钻孔区内充满重生骨小梁结构，8周时钻孔区内骨小梁成熟，小梁有骨髓组织填充。

Microscope examination:in group a,at 2 weeks,a few inflamatory cells appeared in the holes with many osteoblasts and ostoid appeared around the drilled holes.at 8 weeks,marrow formed in the drilled holes in group b,at 2 weeks,there were a large amount of osteoblasts in the drilled holes and some ostoid formed around thc drilled holes.at 4 weeks,the drilled holes were full of trabeculae.at 8 weeks,the trabeculae matured with the marrow appeared in the intertrabecular space.

组织学结果：2周时a组钻孔区出现少许炎症细胞，边缘出现较多成骨细胞并有骨组织形成，至8周时，钻孔区内形成骨髓组织，只在边缘形成骨小梁结构。2周时b组钻孔区有大量的成骨细胞，边缘有较多骨组织形成，4周时钻孔区内充满新生骨小梁结构，8周时钻孔区内骨小梁成熟，小梁有骨髓组织填充。［结论］骨髓基质干细胞对兔股骨头缺血性坏死有良好的修复作用。

Sixty six male Sprague-Dawley rats were randomly divided into control group,and 5 model hepatic fibrosis groups perfused alcoholic,maize oil and pyrazole for 2 weeks,4 weeks,8 weeks, 12 weeks and 16 weeks (11 rats in each group) respect...

结果 与对照组比较，酒精灌胃 4周、8周、12周、16周组大鼠OPN、α SMA表达显著增强并且肝内胶原纤维含量显著增加。正常肝组织中几乎不表达OPN ，在酒精性肝纤维化大鼠模型的建立过程中，从第四周开始中央静脉。。。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。