查询词典 two dimensional

The changes of soluble protein composition during the pre-stage, early stage and advanced stage of cantharidin synthesis in M. calida Palla were analysed by SDS-PAGE and two-dimensional electrophoresis.

本研究采用 SDS-PAGE 、双向电泳技术，分析了苹斑芫菁 Mylabris calida Palla 在斑蝥素合成前期、大量合成早期和末期的蛋白质组成的变化。

The examined subjects were asked to carry out Muller maneuver slowly so that the intrathoracic pressure decreases gradually while the blood flow of the right ventricular outflow tract observed with pulsed Doppler echocardiography at the parasternal short-axis view of the great vessels in the two-dimensional echocardiography.

结果 1。模拟实验胸压变化对血流动力学的影响 3 第四军医大学博士学位答辩论文外周静脉压和模拟胸腔压力变化相同时所引起的右心室容积的变化是相同的；当两者同时同步变化时，右心室容积无变化。模拟胸腔压力变化时，模拟肺血管床和左心室内压力同等程度变化。

TBS had obvious image features on CDE:① The parasternal long axis view of two-dimensional echocardiography （2DE） image showed discontinuous echo of ventricular septal,and the anterior aorta developed from right ventricle while the posterior pulmonary artery straddled on the ventricular septal, which was named 2DE ＂straddle sign＂.

TBS的CDE图像特征：①二维超声心动图（2DE）胸骨旁左室长轴观显示室间隔回声中断，主动脉在前方发自右心室，肺动脉在后方骑跨在室间隔之上，称2DE&骑跨征&。

If so, it can also be regarded as viscoelastic. An adequate amount of data useful for bioengineering has been obtained from one- and two-dimensional tensile tests for fresh pericardia of oxen and those fixed by glu-taraldehyde.

作者对新鲜的和戊二醛处理过的牛心包进行了一维和二维的拉伸，试验结果，得到一些可能对生物工程有用的数据。

The method utilizes petrographical information obtained from two-dimensional thin sections to stochastically reconstruct the 3D model of a stone sample by the theories and techniques of image processing and mathematic morphology.This paper compares quantitatively the reconstructed microstructures...

这种方法利用从二维薄片取得的岩相学图象信息，用图象处理理论技术和数学形态学的方法生成了岩石的三维模型，对重建的三维微观结构和实际砂岩的表面细微结构进行了定量比较，比较显示，这种重建方法得到的三维模型孔隙率与原始图象基本一致，其水平面/纵切面/横切面上的颗粒长径分布与实际岩石有较好的相关性。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

So it is of much significance to study the mechanisms of ovule development and abortion of Chi- nese pine. By combining two-dimensional gel electro- phoresis (2-DE) and mass spectrometry analysis, the pat- terns of gene expression in a specific tissue at a specific stage can be displayed and characterized.

双向电泳和质谱技术结合研究基因表达的蛋白质组技术正越来越广泛地应用到植物研究中，我们将该技术应用到油松胚珠发育的研究中，比较了两种蛋白质提取的方法，发现蔗糖提取方法比传统的TCA 方法更适合于油松蛋白质的制备。

In the first part of this dissertation, the UV-induced photochemically photochromic process of diaryperfluorocyclopentene was investigated using two-dimensional correlation infrared spectroscopy.

二维相关光谱学是光谱分析领域中的最新进展之一，它以动态光谱数据的测定为基础，同时又与实验设计和数据处理相结合。

The mechanism of the UV-induced photochemically photochromic process of 1,2-bis-(2',5'-bimethyl)-3'-thienyl-perfluoro-cyclopentene was investigated using two-dimensional(2D) FTIR spectroscopy.

利用二维相关红外光谱方法首次研究了1，2-二（2'，5'-二甲基-3'-噻吩）－全氟环戊烯在紫外光照引发下发生光化学闭环反应的历程。

This study focused on two-dimensional laboratory model test for the pile-soil-tunnelling interaction using a close range photogrammetric technique and numerical analysis.

使用一个近距离摄影测量的技术和数据分析，这项研究集中于二维堆土壤挖洞互作用的实验室模型试验。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。