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Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点,我们对文献方法进行改进:文献方法用氢氧化钠与3,5-二甲基-4-羟基苯甲酸反应制得3,5-二甲基-4-羟基苯甲酸钠,再用3,5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3,5-二甲基-4-羟基苯甲酸苄酯,我们用氢氧化钾代替氢氧化钠,使合成取得成功;在合成2',6'-二甲基-4'-苯基-吖啶-9-甲酸酯时,文献方法对粗产品进行两次硅胶柱层析纯化,第一次柱层析用氯仿/乙酸乙酯(4∶1,V∶V)作溶剂和淋洗剂,第二次柱层析用己烷/丙酮(2∶1,V∶V)作溶剂和淋洗剂,按照文献方法得到的不是所需要的化合物,我们只进行第一次柱层析纯化,然后用己烷/丙酮(2∶1,V∶V)进行研磨,过滤,洗涤,除去溶于己烷/丙酮(2∶1,V∶V)的部分,得到所需要的产品。

Based on the summary, this paper proposes the urban traffic information system architecture project suitable for the administerial management situation of China. The UTISAP includes a traffic information center, eight subsystems and two databases. Eight subsystems comprise traffic management, ETC, TFGS, traffic information service, GIS, EMS, CVOS and AHS. Two databases comprise the aspects of road traffic management and vehicles management. And the traffic information center is the core.

在总结了国外近年来有关交通信息系统的开发和研究成果后,结合中国在行政管理方面的实际情况,提出了城市交通信息系统的结构方案:一个核心即交通信息中心,交通管理、电子收费、交通诱导、交通信息服务、地理信息、紧急救援、营运车辆管理、车辆安全辅助驾驶八个子系统以及道路交通管理和车辆管理两个数据库,阐述了各子系统的功能。

This whole research constitutes two independent projects, that is "Estimating divergence times among subfamilies in Nymphalidae" and "Fine- and landscape-scale spatial genetic structure of cushion rockjasmine, Androsace tapete, across southern Qinghai-Tibetan Plateau", which discussed respectively the application of molecular evolutionary analysis in two aspects of evolutionary biology.

分子进化分析就是利用分子数据来研究进化问题。地球上呈现的生物多样性则是突变和自然选择的产物,是生物进化的结果。生物进化的过程包含有两个面:基因的长期进化和遗传多样性,也就是通常意义上的大进化和小进化。该项目就是以两个不同的实验——即&蛱蝶科各亚科间分歧时间的估计&和&青藏高原垫状点地梅的空间遗传结构分析&——分别探讨了分子进化分析在大小进化两个方面的应用。

And there are two-part provinces in China whose congregative degrees of Broadcasting Industry are higher than the average level of countrywide.Because of the non negligible political and economical attribute of Broadcasting Industry, the general evaluation to it has to give attention to two aspects, both economical benefit and social benefit.

第9章通过建立广电产业综合竞争力指标体系,采用客观赋权法中的均方差方法,对中国31个省区2000和2004年的广电产业综合竞争力进行了评价,中国31个省级行政区广电产业综合竞争力的强弱差异十分明显,上海、浙江、广东、江苏和北京等省市优势明显,西部的贵州、甘肃等省则竞争力明显落后。

A term that originally referred to the process by which two or more languages converge to form a new language when different cultural groups interact over an extended period, creolization has been gradually extended to describe the transformations undergone in various aspects of culture when two or more groups come into prolonged contact.

最初这个术语指的是当不同的文化群体长时间相互影响后,两种或更多种语言汇合而形成一种新语言的过程。后来这个词的词义逐渐扩展,用来表达两个或更多的群体经过长时间的接触,其文化在诸多方面所产生的变形。

By and large, the influences of the Garden City Theory on city planning of Republic of China mainly had two aspects. The first was planning of new district in city. This was exactly the same with the means by which Howardbuilt two garden cities--Letchworth and Wewyn in suburb in order to avoidincreasingly expansive London. The second was the decentralism of Chinese city planning after the War of Resistance Against Japan. This was tightly correlated with some West decentralization planning theories such "satellite city","organic decentralization","neighborhood unit" which rooted in the Garden City Theory.

就总体影响而言,一是城市新区的规划,这与霍华德避开日益膨胀的伦敦市区,在郊区进行莱切沃斯和韦林两座田园城市的建设做法如出一辙;二是在抗日战争胜利以后,中国的城市规划多主张分散主义,这又显然与田园城市理论及源于田园城市理论的卫星城市、有机疏散、邻里单位等分散主义的规划理论密切相关。

This article believed that, under the new class sign idea teacher role dislodgement and the quality renewal are an eternal process, the role dislodgement and the quality renewal is in the identical developing process unification of opposites two aspects, the two is a condition mutually, complements one another.

本文在综合各家理论研究的基础上,联系中学语文教学实际,概述了教师角色变位的主要形式及素质更新的基本内容,并运用马克思主义哲学的基本原理初步探讨了教师角色变位和素质更新的辩证法。

This article believed that, under the new class sign idea teacher role dislodgement and the quality renewal are an eternal process, the role dislodgement and the quality renewal is in the identical developing process unification of opposites two aspects, the two is a condition mutually, complements one another.

本文认为,新课标理念下教师角色变位和素质更新是1个永恒的过程,角色变位和素质更新是同1发展过程中的对立统1的两个方面,2者互为条件,相辅相成。

This article believed that, under the new class sign idea teacher role dislodgement and the quality renewal are an eternal process, the role dislodgement and the quality renewal is in the identical developing process unification of opposites two aspects, the two is a condition mutually, complements one another.

本文认为,新课标理念下教师角色变位和素质更新是一个永恒的过程,角色变位和素质更新是同一发展过程中的对立统一的两个方面,二者互为条件,相辅相成。

There was significant difference on the two aspects between the two groups(P<0.05).Conclusions:The extraperitoneal sigmoidostomy has the advantages of less complications and better defecation functions.This operation should be applied widely in clinical practice.

腹膜外乙状结肠造口术具有术后并发症发生率低和排便控制功能较好的优点,可提高患者术后的生存质量,值得临床推广。

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You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台,"Mays这样说道。"

F: I think the oval shape suits you well.

我觉得这副椭圆形的可能很适合你。