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In this research, the spider silk from the major ampullate gland of native Nephila pilipes was collected via the forced-silking apparatus at the reeling speed of 3 m/min. The obtained silk was dissolved in hexafluoro-isopropanol. The influence of pH and temperature over the secondary structure of the silk protein in solution state was examined via Circular Dichroism. The effect of silk concentration and electrical field strength on the structure conformation of silk protein during film casting process was investigated via FT-IR. During the drying process, the increase of silk concentration and the presence of electric field tend to form β-sheet and β-turn. Finally, the dried film of silk protein was treated with different temperatures and cation solutions to study the post-treatment effects.

本研究所采用之蜘蛛丝蛋白质系取自本土品种人面蜘蛛之大囊壶腺体，以3 m/min之卷丝速度强迫取丝(forced-silking)收集蜘蛛丝，再将纤维溶於六氟异丙醇溶剂中配制成溶液，以圆二色光谱仪分析在不同 pH 值与温度条件下丝蛋白质在溶液中之二级结构变化，再将蜘蛛丝溶液以不同浓度及电场强度下乾燥成薄膜后，以傅立叶红外线光谱仪检测分析丝蛋白质在固体状态下之二级结构，最后以不同温度加温、离子溶液浸泡等条件对乾燥所得之薄膜进行后处理，并分析其二级结构之变化。

Personal brief introduction 个人简历 Name:Chengnan date of birth:In 1984 September 07th Sex:Male marriage:Single Height:163 CM weight:54KG Graduate a college:Fukien dragon rock a life time section technique school profession:The machine electricity integral whole turns profession The work grow:Molding tool benchwork Oneself graduated from the dragon rock a life time section technique school machine electricity integral whole to turn professional educational system for two years in 2002 the course learn had in the school 《the car work craft 》《the machine graphics 》《the machine foundation 》《the metals material and hot processing 》《the electrician learn 》wait more than ten courses.

个人简历姓名：陈楠出生日期：1984年09月07日性别：男婚姻：未婚身高：163CM 体重：54KG 毕业院校：福建龙岩世科技术学校专业：机电一体化专业工种：模具钳工本人2002年毕业于龙岩世科技术学校机电一体化专业学制两年在校所学课程有《车工工艺》《机械制图》《机械基础》《金属材料与热处理》《电工学》等十几门课程。

So, the details of our buying inkjet printing, in spite of price and quality which is bepaid, but it should also be subject to their means and entrepreneurial regions, are familiar with clients in advertising quality requirements, which in turn purchase one product is right for you.

所以，抽象我们在选购喷绘机的时间，尽管代价和质量这两个方面是次给思考的题目，但还答视乎自己的经济本领和不守业地域、谙习的客户们差告白质量的给求，进而选购一台得当自己搁置的产物。

It was also found that there were two mutants of amino acids in this study, which may impact the normal biological function of FS, and in turn, affects the reproductive abilities in Bubalus bubalis.

研究发现两处氨基酸的突变，这可能影响FS正常的生物学功能发挥，从而影响水牛的繁殖机能。

It was the turn next of the other new arrival, Domoraud, to get in the action when, seizing on a flicked header from Matt Pattison, he strode through on the Woking left to test the Lewes keeper with a left-footed strike. On the quarter hour mark Danny Bunce tried an ambitious effort from 30 yards, the ball passing a foot or so over the bar. A minute later a good move involving Domoraud and Lambu ended in a scramble in the box. The ball reared up but wouldn't come down quickly enough to allow Gez Sole to get the ball on target.

接下来轮到了另一个新加盟的队员，多莫劳德接马特-帕蒂森的头球传球在左路起左脚射门被路易斯队门将化解。15分钟时丹尼-邦斯有一次30米外的劲射，球稍稍高出横梁。1分钟后多莫劳德和朗布在禁区内的配合制造了威胁，但球下坠速度不够，朱塞佩-索勒没能射中目标。

The whole curve appears near S shape curve which revealed that the compound eyes of green lacewinng have strong adapting ability to light stimuli.4 Relation of the dark-adapted time and ERG value indicates that time of day eyes turning to night eyes is not similar in different kind of species. The turn of compound eye from day eyes to night eyes need about 120min in Chrysopa sinica Tjedar,, but is not accord to in different unit the other two species.

以ERG值为指标，在—定时间内，暗适应时间长短影响草蛉复眼的ERG振幅的大小，不同种革蛉其ERG值达稳定所需时间是不—致的，中华草蛉一般都需要120m，ERG值才能达到稳定，而大草蛉、丽草蛉不同个体ERG值达到稳定的时间不完全一致，大草蛉ERG值一般需60-90m达稳定，个别在120m左右达稳定，丽草蛉ERG值一般在60m左右达到稳定。

As to the dynamic loads, the calculation considers only the inertial loads because of jump-start and brake. However, in the actual turn-over processor of the circumgyrate machinery, especially in the process of braking, the impact to the organization is very obvious. Now in the strength calculation of the circumgyrate machinerys parts, this distinct dynamic effect is replaced by safty coefficient.

然而，在回转机构实际的运转过程中，特别是在制动时，机构中发生的冲击振动现象十分明显，但这种非常显著的动态效应在回转机构的强度计算中仅由安全系数来考虑，也就是把较复杂的动态系统人为地转化为简单的静态系统并没有准确地反映事物的本质。

In newly the development progress of 1, Tang's mountain has become the strong dint engine, city ferry that a grain of important pawn, the region industry of"bo triangle" that the northeast second integral whole turns comes together to hope a core city in the city turn, taking to lead the way a sheep function in the construction the circumlittoral economy the society the development the progress of strong province.

在新一轮的发展进程中，唐山已成为东北亚一体化的一粒重要棋子、&渤三角&地区产业聚集的强力引擎、京津冀都市圈中的一个核心城市，在建设沿海经济社会发展强省的进程中担当着领头羊作用。

The IRSL and GLSL age estimates reported for 11 samples of colluvial and slope wash deposits from trench wall at Xiyangfang, Yanqin county, Beijing, could be useful for understanding tectonic activity in the last 20ka, particular in last 2000 years, in turn, for assessing earthquake risk around the Yanqing area.

北京延庆西羊房探坑剖面崩积物和坡面沉积物11个样品的IRSL和GLSL测年数据将有助于对延庆地区近二万年来，尤其近二千年来构造活动的理解和地震危险性评估。

The exterior walls of these atria are composed of minimal membranes supported by two-way cable nets in order to maximize their visual and solar transparency, The main atrium's cable net is stiffened by twoV-shaped cables that in turn are counterweighted and kept in tension by the weght of the suspended lantern.

外墙这些心房组成最小膜支持双向电缆网，以便最大限度地利用他们的视觉和太阳能的透明度，主要心房的有线网是由twoV肋形电缆，又counterweighted和保存在紧张的weght暂停灯笼。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。