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Potato spindle tuber viroid ; hammerhead ribozyme ; cleavage activity in vitro

马铃薯纺锤形块茎类病毒;锤头型核酶;体外切割活性

The genes of hammerhead ribozymes targeted against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式,设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。

The genes of hammerhead ribozymes targeting against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式,设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。通过体外转录,将PSTVd负链RNA分别与双价和三价核酶混合,37℃温育 2h。

Viroids replicate within the plant cell and cause characteristic disease symptoms; examples are the potato spindle tuber viroid and the hop stunt viroid.

类病毒的复制在植物细胞内进行,能够导致典型病症。例如马铃薯纤块茎类病毒和蛇麻草矮病类病毒。

Based on the conserved region nucleotide sequences of four viruses (Alfalfa Mosaic Virus, ALMV; Cucumber mosaic virus, CMV; Cucumber mosaic virus-satellite, CMV-sat; Potato virus Y, PVY) and one viroid (Potato spindle tuber viroid, PSTVd) which could be pathogenic on potato world and one inner control(18S rRNA), specific oligonucleotide probes and primers were designed and spotting for hybridization detection.

利用芯片点样仪将5种侵染马铃薯的病毒/类病毒(苜蓿花叶病毒、黄瓜花叶病毒、黄瓜花叶病毒-卫星病毒、马铃薯病毒Y、马铃薯块茎纺锤状类病毒)的保守区寡核苷酸(Oligonucleotide,oligo)探针和PCR探针点样于玻片,并以植物18S rRNA作为内参照制成基因芯片。

Moreover, the increase of contents of IAA, ABA and JA would inhibit the elongation of stolons, whereas, promote tuber formation.

而内源IAA、ABA及JA含量的增加则会抑制匍匐茎的伸长及促进块茎的形成。

The field experiments were conducted to study on the effect of different N, P2O5, K2O fertilization rate and ratio on nutrient status and tuber yield of potato in three potato production bases. According to traditional application rate, N-P2O5-K2O =300-345-375 (kg/hm2), the fertilization rate of N, P2O5, K2O was decreased to 180, 180, 225 kg/hm2, respectively.

试验分别在甘肃省景泰县、内蒙古自治区达拉特旗和河北省沽源县3个马铃薯生产基地进行,在基地常规N、P2O5、K2O肥施用量分别为300、345、375kg/hm2的基础上,研究改变磷、钾、氮肥用量对马铃薯营养状况、块茎产量以及土壤养分的影响。

ITS Sequence can be used to authenticate the genuineness and identify the varieties of wild resources in the root tuber of P. multiflorum.

ITS序列可对何首乌的道地性做出鉴别,对野生资源品种的鉴定具有较好的分辨性。

According to the dry weight of root tuber and effective components content, treatment C is the best planting density.

综合考虑块根产量与活性成分量两个方面,处理C为最佳种植密度。

With 2.1% of fresh weight, the protein content in potato is the highest among root and tuber crops.

它有益健康,富含碳水化合物,能为人体提供充足的热量。

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