查询词典 tube culture

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

Autologous MSC can be obtained easily, their source in human are plenty, own active proliferation and potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful, the standard technology is culture the cell-ball in centrifuge tube, the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Autologous MSC can be obtained easily,their source in human are plenty , own active proliferationand potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer , but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

软骨组织工程的材料学研究主要体现在两类基质材料方面的进展，一类为天然基质材料，如胶原、壳聚糖、纤维素等；一类为人工合成可降解材料，如PGA、PLA等，但两者在来源、塑形能力及细胞识别信号等方面存在不足。

Objective To explore the isolation and culture conditions of neuroepithelial stem cells from neural tube of embryonic rats and to observe their differentiation characteristics in vitro.

目的探讨大鼠胚胎神经管神经上皮干细胞的分离培养条件，并观察其在体外的分化特性。

After 5 days, we determined the effects of LF-PMF on EPCs' proliferation ability by MTT assay, cell cycle by flow cytometry, migration ability by cell scrath assay and angiogenic potential by tube formation assay and 3D- culture assay.

曝磁5天后，MTT法检测细胞增殖情况，流式细胞仪检测细胞周期，划痕试验检测细胞迁移能力，管状结构形成试验和三维培养检测细胞成血管能力。

Hongshan Culture," U - shaped tube of jade," the true meaning - journals | Free Magazine | E-Magazine ...

红山文化&马蹄形管状玉器&的真正含义-期刊杂志|免费杂志|电子杂志。。。

The results showed that pollen in differentfamily or genus of plant have different requirements for phytohormones.The basicmedium of pollen culture supplemented with 2ppm NAA and GA_3 can promotes thepollen germination in Cruciferae.It has been proved that basic medium supplemtedwith 2ppm IAA and GA_3 has special effect on pollen germination and elongation ofpollen tube of woody plant in Rosace...

结果证明：不同科属的植物花粉对激素的要求不同，如在花粉萌发的基本培养基上加2ppm的NAA和GA_3混合液对十字花科植物花粉有促进作用，加2ppm的IAA和GAa_3混合液对蔷薇科木本植物花粉萌发和花粉管伸长有显著效果，而葫芦科植物的花粉在基本培养基上只要有无机盐离子就可以萌发，激素仍有促进花粉萌发的作用。

Shanghai zhoushou steel co,.ltd is set up in 2003 Enterprise idea obeying \" being eager to make progress all-out , developing \" steadily, developing and expanding "service creates and value " corporate culture , take that professionalization clear and definite manages as core, make effort iron and steel material providing customer with high-quality , low cost, have improved and perfected , have taken form unceasingly be eager to make progress and step-by-step with the seamless steel tube, I-steel , U-steel , angle steel , zinc-coated steel are in charge of , zinc plating steel plate , the professionalization opening a flat waiting to assemble a project with material for the characteristic sell enterprise, In east China, area believes degree even there is bigger notability and higher sincerity in home market.

上海周寿钢铁有限公司成立于二零零叁年，秉承&全力进取，稳步发展&的企业理念，和弘扬&服务创造价值&企业文化，以明确的专业化经营为核心，着力为客户提供高品质、低成本的钢铁材料，不断进取并逐步完善，形成了以无缝钢管，工字钢、槽钢、角钢、镀锌钢管、镀锌钢板、开平板等安装工程用材为特色的专业化销售企业，在华东区域乃至国内市场有较大的知名度和较高的诚信度。

Centrifuge tube aggregate culture and BAI induced medium are effective manners in inducing DTCs to express type Ⅱ collagen.

离心管聚集体培养和BAI诱导培养基是诱导去分化转化软骨细胞Ⅱ型胶原的有效方式。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷