查询词典 tryptophan

Recombinant plasmid pFf-22-Trp operon was successfully constructed, and both the anthranilate synthase and tryptophan synthase activities of expressed product increased. It laid a foundation of construction of recombinant E. coli strain for production of tryptophan in a large scale.

已成功构建了重组表达质粒pET-22b-Trp operon，邻氨基苯甲酸合成酶和色氨酸合成酶的活性在大肠杆菌中得到了提高，为高产色氨酸基因工程菌的构建奠定了基础。

Due to tryptophan's resistance to insulin, this will result in an increase of circulating tryptophan.

由于色氨酸对胰岛素的耐受，结果血液循环中的色氨酸升高。

The inner reorganization energy and the exothermicity of the reaction are then determined. Closed shell HF SCF calculations have been carried out for the systems Tryptophy1-Tyrosine and Tyrosyl-Tryptophan for different values of R, and the Koopmans^,theorem is invoked to estimate the energy level splitting A.From the obtained A~m~i~n, the values of electron transfer matrix element V~D~A are determined to be 0.96kJ. mol^-^1 and 0.87kJ.mol^-^1 for Tryptophyl-Tyrosine and Tyrosyl- Tryptophan, respectively.

对色氨酰酪氨酸和酪氨酰色氨酸体系进行闭壳层HF自洽场计算，按Koopmans定理计算体系分子轨道分裂能值A，在R约为0处发现了A的极小值，从而获得色氨酰酪氨酸及酪氨酰色氨酸体系分子内电子转移的电子转移矩阵元V~D~A分别为0.96kJ.mol^-^1和0.87kJ.mol^-^1。

Through a variety of resin filter, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research, this article eventually determine the strong acid cation resin HZ-001 which is at pH 3, 3BV / h, having the largest adsorption to the tryptophan; the fermentation broth were centrifuged to remove sediment,then treated with activated carbon to be decolored and then treated with strong acid cation resin HZ-001. After that,2mol/l amino acid were passed through the HZ-001 ion exchanging column,kept almost constants when the flow rate stayed 6 BV / h. At 60℃,it was condensed into the saturated state and then added Ethanol until its percentage reaching 30%. At 4 ℃ under 100rpm placed in the shaker 20h, the collection of L-tryptophan was dried up in vacuo.

本文通过对种树脂的筛选、吸附和洗脱条件的优化，以及对脱色、结晶方法的研究与摸索，最终确定强酸型阳离子树脂HZ-001在pH=3，3倍床体积流速，温度室温，对色氨酸有最大的吸附作用；将发酵液离心去沉淀并用活性炭脱色后通过HZ-001强酸型阳离子交换树脂柱，再用2mol/L的氨水6倍床体积流速在室温下进行洗脱，收集洗脱液，60℃浓缩为过饱和状态后加入30%无水乙醇，4℃下100rpm摇起晶20h，过滤搜集结晶并真空干燥得白色粉片状L-色氨酸晶体。

Results Agarose gel electrophoresis proved that the length of PCR product was about 7 000 bp. SDS-PAGE showed that the target protein was highly expressed. Compared with those of control, the anthranilate synthase and tryptophan synthase activities of expressed product increased by 2.7 and 3.2 folds respectively.

结果 凝胶电泳可见代PCR扩增产物大小约为7000bp，SDS-PAGE鉴定目的蛋白分别得到了表达，邻氨基苯甲酸合成酶和色氨酸合成酶的活性分别比对照提高了2.7和3.2倍。

In this study, a feedback insensitive anthranilate synthase gene ( ASA2 ) cloned from a tobacco cell line was tested for Agrobacterium mediated transformation of axis tissue of soybean mature embryo, with a tryptophan analogue 5 methyltryptophan (5 MT) as the selective agent.

通过从烟草中克隆的邻氨基苯甲酸合成酶基因(ASA2 )作为筛选标记基因，并采用氨基酸的类似物 5 甲基色氨酸为筛选剂，进行了农杆菌介导的大豆成熟胚尖转化研究。

There is fluorescence resonance energy transfer between the intrinsic tryptophan and MIANS that labeled on Cys residues of ArsA, antimonite can increase the efficiency of energy transfer.

ArsA内源色氨酸和标记于Cys上的MIANS之间存在荧光能量共振现象，SbO〓能够增加它们之间的能量共振转移效率。

The results indicated that all tested strains could produce 0.83~8.41 mg/kg of tyramine, putrescine, cadaverine, histamine, spermine and spermidine, but none of them could produce tryptophan-amine in sausage fermentation, the biogenic amines' content in sausage was dependent on strain obviously.

结果表明，供试乳杆菌和片球菌均能产生0.83~8.41mg/kg不等量的组胺、尸胺、腐胺、酪胺、亚精胺和精胺，但都不产生色胺，产品中的生物胺生成量表现出明显的菌株效应。

The kinetic analysis indicated that one tryptophan and one carboxyl group in one molecular of XynA were essential for the XynA activity.

XynA的化学修饰动力学分析表明，每个酶分子中有 1个色氨酸残基和 1个谷氨酸残基对酶的活性特别重要。

Under optimal conditions mixtures containing eight inorganic anions, six amino acids such as arginine, histidine, cysteine, methionine, tryptophan and proline with positive or negative charge and four basic proteins such as lysozyme, cytochrome c,α-chymotrypsinogen A and myoglobin were separated completely.

经优化，用中等浓度的中性缓冲液分别成功地分离了八种不同的阴离子Br〓、NO〓、NO〓、SCN〓、MoO〓、BrO〓、WO〓和IO〓；六种带不同电荷的氨基酸如精氨酸、组氨酸、半胱氨酸、甲硫氨酸、色氨酸和脯氨酸；四种碱性蛋白质如溶菌酶、细胞色素c、抗凝乳蛋白酶原A和肌球蛋白。

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。