查询词典 trypsin

PurposeTo evaluate the abilities to reduce blood glucose and resist trypsin enzymolysis of chemical modified insulin.

目的评价化学修饰胰岛素的降血糖作用及抗胰蛋白酶降解的能力。

Through researches of stability to heat,acid and alkali, the RMPE had an activity to epiphyte up to 100℃, and inhibited epiphyte most strongly at pH7~8. RMPE were sensitive to proteinase K and trypsin.

对该蛋白提取物的稳定性测定表明，加热到90℃未丧失活性，对酸碱的适应范围也较大，在pH 7～8的时候抑菌效果最为明显，对蛋白酶K和胰蛋白酶敏感。

The author first reported the social phenomenon and synchronizing development incryptosporidia,the dynamic process of the sporozoites or merozoites aparted from theirparent substances and developed into trophozoites,the culture of C.baileyi in chickenembryo,and the excysting process without both the trypsin and the sodium taurocholatein 37℃ water.The high levels of excystation(60%)oceured at 37℃ water showedthat temprature was very important in cryptosporidia excysting process.

在国内首次观察和描述了薄壁型隐孢子虫卵囊；首次报道隐孢子虫具有群体寄生现象和同步发育特征；首次描述了隐孢子虫的裂殖子或子孢子离开母体和发育为滋养体的动态变化过程；首次应用粪便中纯化的C.baileyi卵囊感染鸡胚成功，并用37℃水脱囊，其脱囊率高达60％，表明温度对隐孢子虫的脱囊具有重要作用，无需胰酶和牛胆酸钠的参与。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

We compared the number of integral membrane proteins identified from the PM of human CNE1 cells by PAOT with that of an optimized trypsin/chymotrypsin method. More than double the total number of identified IMPs was obtained with PAOT, mainly contributed by the IMPs with positive grand average of hydropathicity scores.Part 2: We describe a procedure for digesting the hydrophilic domains in the absence of detergents and denaturants.

同时，我们以人类CNE1细胞系为材料，比较了PAOT与另外一种优化好的胰蛋白酶/胰凝乳蛋白酶酶解方法，发现PAOT鉴定到的整合膜蛋白是胰蛋白酶/胰凝乳蛋白酶酶解的2倍，并且这些整合膜蛋白的疏水值大部分都为正值。

The results showed that the shrimp hemocyanin expressed phenoloxidase activity,whose A490nm increased after being activated by trypsin.

但目前研究已在对虾中发现了一些重要的免疫因子如酚氧化酶原激活系统、凝集素、抗菌肽等。

Result succinylation modified, trypsin hydrolysis, SDS and β-mercaptoethanol treatment greatly reduced lectin from gill of bighead carp; dimethyl sulfoxide, NaCl, tri fluoroacetic acid at a given treated concentration had no effect on its activity; lectin from gill of bighead carp agglutinated Haenatcoccus pluvialis, vibrio harveyi and Vibrioauguil larum specifically.

结果］琥珀酰化修饰、胰蛋白酶水解、SDS及β-巯基乙醇处理均使鳙鳃凝集素活性大大降低；在设定的处理浓度下，二甲基亚砜、NaCl及三氟乙酸对其活性没有影响；鳙鳃凝集素能专一地凝集雨生红球藻及哈维氏弧菌和鳗弧菌。

Processing with SCCO2 raises the content of LOX enzymes by 99% and inhibit trypsin activity responsible for off-flavors.

经过SCCO2处理的大豆可抑制胰蛋白酶抑制素。这些经过处理的豆类寿命在不进行冷冻的情况下可达到30天。

Acquire newborn rat astroglial monolayer and dissect embryonic 18 d rat hippocampus and obtain single cell suspension through trypsin digestion. plate the suspension to poly l lysin coated coverglass in（2-5）×103 ?

首先以新生大鼠为实验材料培养纯化单层星形胶质细胞，随后以孕18 d胎鼠为实验材料，分离海马皮层，经胰酶消化制备单细胞悬液。

Acquire newborn rat astroglial monolayer and dissect embryonic 18 d rat hippocampus and obtain single cell suspension through trypsin digestion. Plate the suspension to poly L lysin coated coverglass in（2-5）×103 cm-2 density.

首先以新生大鼠为实验材料培养纯化单层星形胶质细胞，随后以孕18 d胎鼠为实验材料，分离海马皮层，经胰酶消化制备单细胞悬液。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。