查询词典 trypsin

The main collagens in skin are type I and III. In this research, total collagens were separated from rat skin by enzymatic digest and acetum methods. The collagens were denatured at 60℃ and digested with trypsin. Characteristic peptides typical for collagen type I and III were identified with high performance liquid chromatography/mass spectrometry.

哺乳动物皮肤真皮中胶原蛋白含量约为70%，主要为是I型、III型胶原蛋白，本实验利用稀酸溶解和酶法提取了大鼠皮肤中的总胶原蛋白，将胶原蛋白粗提品在60℃变性后用胰蛋白酶进行降解，液相色谱/质谱联用法分析了两种胶原蛋白的特征多肽，利用特征多肽比较了不同生长期大鼠皮肤中I型和III型胶原蛋白相对含量。

METHODS: BMSCs, CCs, and ADAS were primary-cultured from goat bone marrow following trypsin digestion and amplified in monolayer.

抽取羊骨髓，胰酶消化后获得原代骨髓间充质干细胞细胞、软骨细胞和脂肪源性成熟基质细胞，单层培养扩增。

The different effects of water-miscible organic solvents on the esterase and amidase activities of trypsin have been known for some time.

本文首次报道在单相共溶剂体系通过溶剂的疏水作用对木瓜蛋白酶的酰胺酶活性和酯酶活性进行调控。

We chose a-amylase which is important in digestion of amylum and saccharide as well as pepsin and trypsin that play key role in digestion of proteins.

我们选择了在消化淀粉和糖类物质中起重要作用的α-淀粉酶以及在蛋白质消化过程中起主要作用的胃蛋白酶和胰蛋白酶。

The affection of the autolysis of the peptide near active site on trypsin activity has also been analysed.

我们还分析了EPT活性中心部位附近肽键的自溶对猪胰蛋白酶的活性的影响。

This study is design and preparation of active peptide analogues of mung bean trypsin inhibitor Lys fragment, Trichosanthes trypsin inhibitor I, carcinoembryonic antigen peptide and aspartame using solid and liquid phase peptide synthesis methods.

本论文研究活性多肽类似物的设计、制备和功能，分为四个部分，采用多肽固相和液相合成的方法，分别合成了活性多肽绿豆胰蛋白酶抑制剂 Lys片段长链16肽、天花粉胰蛋白酶抑制剂、癌胚抗原肽、甜味肽等一系列类似物，并测定了这些类似物的生物活性，有以下新的研究结果：1、furin酶抑制剂的设计、制备和功能；2、芳香氨基酸芳环之间的π-π共轭能部分补偿二硫键的作用；3、癌胚抗原肽Gly4被L-Pro替代可增加与HLA-A2分子的结合；4、肽键对甜味肽保持甜味是必须的。

Trypsin treatment resulted in a remarkable decrease of the PsbS protein amount in the thylakoids from saturating light-preilluminated but not in those from dark-adapted soybean leaves , compared with that of the control (from dark-adapted soybean leaves and without trypsin-treatment) thylakoids .

与对照（从暗适应大豆叶片提取、没有用蛋白酶处理的类囊体，DU）相比，用胰蛋白酶处理从饱和光照射大豆叶片提取的类囊体后，类囊体的PsbS量显著降低，但是用胰蛋白酶处理从暗适应的大豆叶片提取的类囊体后，类囊体的PsbS量没有显著变化。

Greater than 40 serial MSC passages and 100 cell population doublings have been recorded for some MSC batches. Early and late passage MSCs were defined here as those cultures receiving less than 5 trypsin passages and more than 15 trypsin passages, respectively.

本实验研究了传代超过40代的MSCs和100个细胞集落，并将传代少于5次和多于15次的分别命名为传代早期和晚期的MSCs。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

The trypsin inhibitor activity of sporamin was determined using the method of Smith, and show a fairly linear relationship with the concentration of sporamin between an region of the inhibitor rate from 20% to 70%. The R^2 was 0.983 and the trypsin inbitor activity was (735.32±12.11) TIU/mg.

采用Smith推荐的方法测定了sporamin的胰蛋白酶抑制剂活性，拟合分析结果表明：在对胰蛋白酶抑制率为20%~70%的范围内，胰蛋白酶抑制率与sporamin的浓度呈线性关系，回归系数R^2为0.983。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。