查询词典 trypsin

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

The protein in raw eggs also contain a mucinous protein, it can also be combined and trypsin impede the decomposition of proteins.

生鸡蛋中还含有蛋白质粘液蛋白，也可以结合和胰蛋白酶阻碍蛋白质的分解。

Phenylmethylsulfonyl fluoride (174.2 mg/mL), chicken ovomucoid (1000 mg/mL) and soy-bean trypsin inhibitor (1000 mg/mL) could inhibit enzyme activity, which indicated that the enzyme belonged to serine protease group. On plasminogen-free fibrin plates and plasminogen fibrin plates, the fibrinolytic activity had no obvious difference, indicating that the enzyme was a fibrinolytic enzyme which degraded fibrin directly, but not a plasminogen activator which degraded fibrin by activating plasminogen.

此菌株产生的纤溶酶在50℃以下和pH5.0~11.0范围内具有较好的稳定性，最适作用温度为42℃；最适pH值为9.0；Mg2+、Ca2+对此酶有明显的激活作用，而Cu2+能完全抑制酶的活性；174.2 mg/mL的苯甲基磺酰氟、1000 mg/mL的鸡卵类粘蛋白和1000 mg/mL大豆胰蛋白酶抑制剂能完全抑制酶活性，初步说明此酶属于丝氨酸蛋白酶类；体外溶纤作用表明，该酶溶解纤维蛋白的方式是直接溶解，而不是通过激活纤溶酶原。

The HE activity was almost completely inhibited by SBTI, p-APMSF, bestatin and NEM, greatly inhibited by ovomucoid, TLCK, IAM, chymostatin and PMSF, and slightly inhibited by pepstatin A, TPCK, LBTI and leupeptin. And these results imply that HE is most probably a trypsin-type serine protease.

抑制剂对其酶活性的影响结果显示，该孵化酶的活性几乎可被SBTI、p-APMSF、Bestatin和NEM所完全抑制，被ovomucoid、TLCK、IAM、chymostatin和PMSF所强烈抑制，而对pepstatin A、TPCK、LBTI和leupeptin不敏感，表明该酶极可能是一种属于胰蛋白酶类型的丝氨酸蛋白酶。

Periploca sepium Bunge;protease;trypsin-like;chymotrypsin-like;lipase;amylase

杠柳；蛋白酶；类胰蛋白酶；类胰凝乳蛋白酶；脂肪酶；淀粉酶

A trypsin inhibitor was isolated and purified from Phaseolus vulgaris, and partial characterization of PVTI was studied.

本论文以菜豆的一个栽培品种紫花芸豆为材料，从中分离纯化胰蛋白酶抑制剂PVTI，并对其部分性质进行了研究。

This paper intends to give an overview of the production and catalytic mechanism of trypsin, the biochemical characteristics, gene cloning, ontogeny, and expression and regulation of piscine trypsins.

本文综述了胰蛋白酶生成、催化机制以及鱼类胰蛋白酶的生化特征、基因克隆、个体发生及表达调控等。

Methods:Cardiac muscle of twoday neonate rattus was digested repeatedly by 0.0625% trypsin.The cardiocytes were collected in DMEM medium,which contains 10% fetal bovine serum.Myocardial cells were isolated with the technique of differential anchoring velocity with 90 min and were pured by Brdu.The cardiocytes were placed into CO2 incubator to incubate seven days after their purification.

应用0.0625%的胰蛋白酶重复消化出生第2 d乳鼠的心肌组织多次，收集的细胞用含10%胎牛血清的DMEM培养基中和，用差速贴壁分离法分离，在以溴脱氧尿嘧啶纯化心肌细胞后置CO2培养箱孵育7 d。

Trypsin comes from pancreas , intestines and pyloric ceca of fish .

胰蛋白酶来源于鱼类的胰脏，肠道和幽门盲囊。

The optimal conditions as follows: the digestion solution with 0.1ug/ul of trypsin was deposited in section, sonic oscillation for 10 minutes at 60℃; using the sinapic acid as the MALDI matrix.

实验结果表明，质谱出峰数明显增加，质谱信号明显增强。说明我们建立的新方法适用于福尔马林固定组织的多肽谱分析。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。