查询词典 trypsin

Firstly , the V4 stock was purified three times by plaque formation. It's difficult to see clear plaques in CEF cells due to the avirulent characteristic , so we treated the V4 stock with trypsin and then allantoic Fluid and Mg2+ were added in the layer gel. In this case, we could see small plaques occasionally, but not stably.

本实验首先对实验室保存的V4株进行了三轮的蚀斑纯化，由于V4株属无毒株，其在CEF细胞中很难形成可见的蚀斑，因此我们采用了用胰酶处理V4病毒，并在上层胶中添加尿囊液及Mg2+，这样可以偶尔产生可见的蚀斑，但实验结果重复性很差。

The model includes one β-trypsin, two amylamine molecules, four sulfates, one calcium, and 135 water molecules.

结果表明，正戊胺于环已烷中和β-牛胰蛋白酶有强结合作用，一个戊胺分子结合到酶活性中心；另一个结合在β-牛胰蛋白酶的表面，与也被结合的硫酸根形成氢键。

In aqueous solution no binding observed in crystal structure. In cyclohexane, one amylamine molecule is observed in the active site of trypsin and more sulfates are also found on enzyme surface, which are further proofs that electrostatic interaction are stronger in organic solvent.

在水溶液中，晶体结构难以观察到戊胺与牛胰酶的结合，在环己烷中，一个戊胺分子结合在胰酶的活性中心，同样酶表面观察到了更多的硫酸根，这些都进一步验证了有机溶剂中静电相互作用加强。

The X-ray structures of the crystals of bovine pancreatic β- trypsin soaked with amylamine as a probe were studied and compared in cydohexane and aqueous solution, respectively.

巫广腾；黄其辰；朱广宇；钱民协；唐有祺摘要：丝氨酸蛋白酶是有机溶媒中研究酶促反应的主要对象之一。其中的胰蛋白酶在生物体内有重要的作用，对其抑制剂如苯甲脒衍生物的研究可应用于治疗很多疾病。

It performs autodigestive process resulting in pancreatic and systemic reactions after trypsin is activated.

在外科急腹症中它的发病率仅次于急性阑尾炎、急性肠梗阻、急性胆囊炎和胃、十二指肠穿孔，是普通外科常见急腹症之一。

Binding affinities of a series of benzamide inhibitors for trypsin were evaluated by molecular dynamics simulations using a linear response approach.

用基于线性响应近似的自由能预测方法计算胰蛋白酶和苯酰氨类抑制剂的结合自由能。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

The experiment two: enzyme preparation significantly improved average daily gainand feed conversion ratio (P＜0.05). Enzyme preparation significantly increased energymetabolizability and digestibility of crude fiber, crude protein and neutral detergent fiber,but had no remarkable effect on digestibility of dry matter, crude fat and acid detergentfiber. Enzyme preparation significantly decreased the relative viscosity of duodenal andjejunal digesta. The pH of intestine had no noticed difference in all groups. Enzymepreparation significantly decreased relative weight of gizzard, proventficulus, duodenum,jejunum and ileum. Enzyme preparation significantly increased villus size of duodenumand jejunum, and villus to crypt ratio of duodenum and ileum significantly increased too.Enzyme preparation considerably decreased ileal crypt height (P＜0.05), and didn"t affectthickness of intestinal wall. Supplementing enzyme preparation, the serum glucose, totalprotein and alanine aminotransferase, but enzyme preparation hadn"t noticed influenceupon uric acid, total cholesterol, triglyceride and high-density lipoproteins. Enzymepreparation significantly increased insulin, triiodothyronine and insulin-like growthfactor-Ⅰ. Adding enzyme preparation, the percentage of thyroid stimulating hormone andgrowth hormone in the serum increased 16.44%, 19.18% and 18.84%, 21.74%respectively, and the percentage of glucagon and thyroxine decreased 12.07%, 14.36% and 13.79%, 15.40%, but failed to reach statistical significance (P＞0.05). Enzymepreparation significantly increased (P＜0.05) the trypsin and amylase activity of duodenaland jejunal digesta, but enzyme preparation didnt affect significantly (P＞0.05) theintestinal lipase activity and pancreatic digestive enzyme. Enzyme preparation had nosignificant effect on caecal microbial population.

试验二：酶制剂显著提高平均日增重和饲料转化率(P＜0.05)；酶制剂显著提高能量代谢率及粗纤维、粗蛋白、中性洗涤纤维消化率(P＜0.05)，而对干物质、粗脂肪、酸性洗涤纤维消化率影响不显著；酶制剂显著降低十二指肠和空肠食糜相对粘度(P＜0.05)；添加酶制剂对肠道pH影响不显著；酶制剂显著降低肌胃、腺胃、十二指肠、空肠、回肠相对重(P＜0.05)，显著提高十二指肠和空肠绒毛高度，显著增加十二指肠和回肠绒毛高度／隐窝深度，降低回肠隐窝深度(P＜0.05)，对肠壁厚度影响不显著；酶制剂显著提高血清葡萄糖、总蛋白和谷丙转氨酶浓度(P＜0.05)，对尿酸、总胆固醇、甘油三酯及高密度脂蛋白浓度影响不显著，显著提高胰岛素、T_3、IGF-Ⅰ水平，添加酶制剂后，促甲状腺激素、生长激素分别提高16.44％、19.18％和18.84％、21.74％，胰高血糖素和T_4分别降低12.07％、14.36％和13.79％、15.40％，但差异不显著；酶制剂对胰腺消化酶活性影响不显著，显著增加十二指肠和空肠胰蛋白酶、淀粉酶活性，对小肠脂肪酶活性影响不显著；酶制剂对盲肠微生物菌落数影响不显著。

Canavalia ensiformis protein has relatively high nutritional value,but the unprocessed Canavalia ensiformis protein contains a number of anti nutritional factors such as hemagglutinin ,trypsin inhibitors that limit its used as human food and animal feed.

洋刀豆蛋白是一种具有较高营养价值的植物蛋白质，但是其中含有的一些抗营养因子，如血球凝集素(ConcanavalinA ，简称ConA)、胰蛋白酶抑制剂等，限制了它在人类食用植物蛋白和动物饲料中的应用，其中ConA是最主要的抗营养因子。

In this thesis, four methoxypolyethylene glycols (MPEG, molecular masses 350, 750, 2000 and 5000 Da), each activated by nitrophenyl chloroformate, were used to modify trypsin.

本文用四种不同分子量的MPEG（分子量分别为350、750、2000和5000）对胰蛋白酶进行化学修饰实验研究，MPEG活化方法采用对硝基苯甲氧酯法。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。