查询词典 truncated sequence

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

A series of methods and software programmes for generating truncated Gold sequence are brought forward in this paper, the correlation and balance characteristics of different truncated proportion Gold sequence have been analysed through the simulation of truncated Gold sequence performance, involve with before and after truncated, different period of sequence and different truncated length.

在本文中，研究了截短平衡Gold序列的产生方法，给出了生成截短平衡Gold序列软件程序，通过对截短前后、不同序列周期、不同截短长度的截短Gold序列的性能仿真，分析了不同截短比例Gold序列的相关特性和平衡特性。

Truncated fragments of the amplified sequence were then subcloned into the 5＇of eukaryotic expression vectors with luciferase or chloramphenicol acetyltransferase gene as reporter genes.

将它们进行删切后在荧光酶和氯霉素乙酰基转移酶报告基因真核表达载体的上游构建了18个亚克隆并分别转染Jurkat细胞。

The change phase is estimated by window and interpolation algorithm, and then a phase compensation function can be built accordingly. By multiply this function and the data sequence from the IFFT of the truncated data sequence spectrum in the time domain, the spectral leakages can be compensated when the new data sequence is transformed into the frequency domain by the DFT.

利用加窗插值的方法估计出该变化的相位，并利用估计结果构造出一相应的反相位函数，在时域上将这个反相位函数与由傅立叶反变换产生的离散序列相乘，得到新序列再通过一次傅立叶变换就可达到频谱校正的效果。

Methods:The whole mature protein coding sequence of three truncated dystrophin gene was amplified by RT-PCR method applied to human muscle cDNA. The fragment was inserted into prokaryotic expression vector pET28a plasmid.

以人肌肉组织cDNA为模板，采用RT-PCR扩增三个截短的肌营养不良蛋白cDNA，分别克隆入原核表达载体pET28a中，经限制性内切酶双酶切及DNA序列分析鉴定目的基因。

Using of matrix assisted laser desorption/ionization time of flight mass spectrometry coupled with carboxypeptidase Y digestion for the analysis of C terminal peptide ladder by direct determination of the mixture of truncated peptide, the sequence of C terminus can be read out simplely from the mass differences between adjacent peptide peaks on the mass spectrum.

用羧肽酶Y水解蛋白质和多肽，结合飞行时间质谱测定酶解后缩短肽的质量，根据相邻两肽峰的质量差别读出蛋白质和多肽的C端序列，在pmol水平下测定了人促肾上腺皮质激素片段以及人血管紧张肽片段，其中人促肾上腺皮质激素片段测到了C端 15个氨基酸残基的顺序

Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。

In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列，并去除5'端9 bp的序列，使N端缺少对肠激酶(Enterokinase， EK)具有抑制作用的脯氨酸，将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒，构建重组表达质粒。

There are three features in the minibasins induced by gravitational sliding on the slope: the firstly, feature is surface of unconformity developed by onlap on minibasin margins or surface of unconformity truncated by mass transport-complex are sequence boundary in the study area; the secondly , accommodation of gravity flow is controlled by up lift rate and sedimentation rate , and styles of filling in the minibasins are dominated by the interrelationship between the two factors; and the third , there are two stacking patterns that consists of upward of turbidite fan-hemipelagic drape mud or mass-transport complexes-turbidite fan-hemipelagic drape mud.

认为在深水陆坡重力滑动作用所形成的微盆地内，层序边界表现为微盆地边缘上超不整合面或重力流对下伏地层的侵蚀不整合；逆冲构造隆升速率与沉积速率共同控制了重力流可容空间和沉积充填样式；沉积垂向演化以块状搬运复合体一浊积扇－深海披覆泥或浊积扇－深海披覆泥为特征。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。