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Extracted DNA by CTAB, in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction, Tris-Phenol (Stabilized with 0.1M Tris, pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction, during crude DNA used to Isopropanol DNA and deterged by DNA detergent, without using RNase remove RNA, we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.

用20%聚乙二醇(polyethylene glycol,PEG6000)模拟干旱,采取CTAB提取缓冲液,按一定比例加入PVP和β-巯基乙醇,用Tris平衡酚-氯仿-异戊醇除去蛋白质及杂质,在粗提时用异丙醇沉淀DNA,并用DNA洗液(10mmol/L醋酸铵,75%乙醇)洗涤DNA,在纯化时用乙醇沉淀DNA,且没有加入RNase去除RNA的方法,分别提取2个品种正常和干旱胁迫的苹果属叶片总DNA,并对提取的DNA进行琼脂糖凝胶电泳和紫外分光光度计检测。

Through the field test in the 103 working face of Shanxi Heshun Tianchi Energy Co.,Ltd., it is showed that the degasification parameter of relieved methane is reasonable. The paper can provide a certain theoretical and technological foundation for optimizing achieving degasification system of relieved methane and safety and simultaneous extracting of coal and methane.

通过山西和顺天池能源有限责任公司103综放面的现场实验,说明所得到的卸压瓦斯抽采布置参数较为合理,保证了工作面的安全生产,为优化卸压瓦斯抽采系统、实现煤与瓦斯安全共采提供了一定的理论依据和技术支持。

In this study,we analyze several calculation methods of the production rate of methane in rubbish and make a detailed calculation of the production rate and the cumulate output of methane. On the basis of this study,we use a real rubbish dumping field to calculate the production rate and the cumulate output of methane.

本文比较分析了几种常用的有关垃圾场甲烷气产率计算方法,并详细计算了甲烷的产率及累计产量,在此基础上用一个比较实际的垃圾填埋场计算垃圾填埋过程中甲烷气的产率情况及累计产量,为垃圾填埋场的沼气发电提供了可靠的资料。

According to the system characteristic of the household methane digesters and the combustion characteristic of methane, a series of household methane generators were designed and manufactured ,the power respectively being

根据农村户用沼气池的系统特征和沼气的燃烧特性,设计研制了功率分别为700,1800,3800,4800W等系列的户用沼气发电装置。

According to the system characteristic of the household methane digesters and the combustion characteristic of methane,a series of household methane generator s were designed and manufactured,the power respectively being

根据农村户用沼气池的系统特征和沼气的燃烧特性,设计研制了功率分别为700,1800,3800,4800 W等系列的户用沼气发电装置。

In recent years, the demand growth rate of methane chloridate beyond the production growth rate in domestic market, there are more than 10 methyl chloride factories in our country, with the general production capability about 70 thousand tons, but the consumption is near about 80 thousand tons, the domestic market demand of monochloro methane reached 120—140 thousand tons in 2006, but the monochloro methane production capability just only 80-100 thousand tons.

近几年,国内市场对甲烷氯化物产品的需求增长速度大大快于生产增长的速度,我国现有氯甲烷生产厂10多家,总生产能力约7万吨,消费量近8万吨,到2006年国内对一氯甲烷的总需求量达到12-14万吨,而时国内一氯甲烷的生产能力8-10万吨。

We found the symmetry was destroyed or decreased in the crystal structure of symmetrical star-shaped molecules, such as tetra (4-cyanophenoxy methyl -methane, tetra (N-pyrrolemethyl) methane, and a center of charility was self-formed. The phenomena was discussed by analyzing the intermolecular hydrogen-bonding interactions of tetra (4-cyanophenoxy methyl -methane.

本文发现,原本高度对称的星状分子,在晶体结构中,分子对称性破坏或降低,表现为手性分子;以四(4-氰基苯氧基甲基)甲烷为例,从分子间氢键作用的角度,解释了该现象形成的原因。

Technology program: there are two methods to produce methane chloridate, one is methane chlorination which methane as the original material, the other is methanol hydrochlorinate which methanol as the original material.

技术方案。生产甲烷氯化物的原料路线主要有两种:以甲烷为原料的甲烷热氯化法和以甲醇为要料的甲醇氢氯化法。

In America, the methane chloridate are all produced by methanol hydrochlorinate; in Europe, there are only three factories to produce methane chloridate by methane chlorination and in Japan, only two factories.

随着甲醇工业的迅速发展,甲醇氢氯化法在经济上和技术上的优势越来越明显,在世界范围内已有后来居上取代甲烷法的趋势。在美国目前甲烷氯化物生产全部使用甲醇法,在欧洲只有3个工厂,日本只有2个厂还在使用甲烷热氯化法。

The author pointed out that generated CBM should consist of primary biogenic methane gas in lignite stage, thermogenic methane gas in long-flame coal to gas/fat coal stage, and deuterogenic biogenic methane gas in coal seam imbedded stage.

首先,根据下侏罗统八道湾组和中侏罗统西山窑组煤储层发育、煤岩、煤质、煤级、煤类、煤相地质特征论述了本区低煤级煤层气生成的物质基础。

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推荐网络例句

However it can often be frust ating for a child when people are unable to pronounce their name.

但它往往可以令人沮丧的一个孩子当人们无法断言他们的名字。

All these compounds were obtained from this genus for the first time. Compound, 4-cumylphenol, was a new nature product.

所有化合物均为首次从本属海藻中分离得到,其中4cumylphenol为新天然产物。

But this is unbelievable .too much.

但是这太令人难以置信。