查询词典 tributyrin

The activity of lipasc from S78- pVT102U/a -lip was up to 20U/ml after 72h fermentation; The product of S78-pVT102U/a-L-lip07 was little, only formed the halo on the tributyrin plate.

其中，重组酵母S78-pVT102U／α-lip摇瓶发酵72h，酶活可达20U／ml；S78-pVT102U／α-L-lip07表达的脂肪酶很少，表达产物仅在三丁酸甘油酯平板上形成透明圈。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

In this study, isolate 23 microbes with lipase activity from Wu-Jie scrap heap at Ilan and Taichung Environmental Protection Section. When the microbes incubator in 50oC, there are isolates B61 and M63 that lipase activity are better than others. Brevibacillus borstelensis SH168 isolated from NTU resterant food waste compost is used as control check. Isolates B61, M63 and Brevibacillus borstelensis SH168 determine the lipase activity by the LB with tributyrin. The lipase activity of isolates B61 and M63 are 2~3 fold higher than Brevibacillus borstelensis SH168. So isolates B61 and M63 are more useful in producing lipase. Isolate B61 can grow at pH 5~9, and at temperature 20oC ~ 60oC; isolate M63 can grow at pH 6~10, and at temperature 30oC ~ 50oC. Isolates B61 and M63 were identified by 16S rRNA. Isolate B61 is Bacillus circulans, and isolate M63 is Bacillus species.

本研究，於宜兰五结垃圾场和台中市环保局厨余堆肥样品中共分离出 23 株具脂肪酶活性菌株， 23 株菌株中经过三油酸甘油酯的洋菜培养基於 50℃培养 5 天后，发现有 2 株菌的脂肪酶活性较强，分别为分离株 B61 和 M63；以本实验室先前自台大女九餐厅蔬果厨余堆肥中所筛选出的耐高温脂解菌 Brevibacillus borstelensis SH168作为本实验对照菌株，将分离株 B61、M63 及 Brevibacillus borstelensis SH168 3 株菌株於含有三酪酸甘油酯液态培养基培养并测定其脂肪酶活性，发现分离菌株之活性皆比 Brevibacillus borstelensis SH168 高出 2~3 倍，认为分离株 B61、M63 适於产生脂肪酶的菌株，分离株 B61 在 pH 5~9 皆可生长，生长温度介於 20 oC ~ 60oC；分离株 M63 在 pH 6~10 可生长，生长温度介於 30 oC ~ 50oC； 2 株分离菌株以 16S rRNA定序结果分离株 B61 为 Bacillus circulans，分离株 M63 为 Bacillus 属。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。