查询词典 tributyrin

The most common methods used for screening of lipase-producing strains are observation of transparent circle on tributyrin agar plate and color-changing circle on olive oil agar plate.

在脂肪酶产生菌的筛选中，最常用的方法是三丁酸甘油酯琼脂平板透明圈法和橄榄油琼脂平板变色圈法。

However, the recombinant yeast could not grow on the traditional tributyrin plate because this kind of medium is lack of essential nutrients for yeast growth.

而传统的三丁酸甘油酯平板缺乏有效营养成分，重组酵母无法生长；橄榄油平板灵敏度较低，低脂肪酶活力的重组酵母不能产生变色圈，这给产脂肪酶基因工程菌的鉴定带来了困难。

In conclusion, the mechanism of differentiation and apoptosis in NB4, K562 cells induced by tributyrin may associate with its up-regulation of acetylated histone and p21(superscript WAF1) mRNA level.

TB诱导NB4、K562细胞发生分化和凋亡的机制与TB引起细胞组蛋白高乙酰化和继而上调p21(上标 WAF1)表达有关。

We use plate to check the lipase activity ,the lipase activity is very low, Compared to the wild type , the mutant could not form the clear halo on the olive oil plate but tributyrin plate, it showed that the 227 th Serine was essential for the lipase activity.

与野生型相比，突变体脂肪酶的酶活很低，不能在橄榄油平板上形成透明圈，但可以在以三丁酸甘油酯为底物的平板上形成透明圈，表明第227位丝氨酸可能对脂肪酶酶酶活具有重要影响。

We detected the distribution of lipases in 35 Aeromonas isolates by PCR andphenotypic test using tributyrin as substrate. The positive rates were 100% and 83%,respectively. The lipase activity or associated genes could be detected in the two avirulentstrains, A. hydrophila W1 and A. hydrophila 4332. It suggested that lipase might not be animportant factor in pathogenic mechanism of A.

对本实验室保存的35株气单胞菌分离株进行了脂酶的表型检测和PCR检测，结果分别为83％和100％，特别是嗜水气单胞菌无毒株W1和Ah4332也能检测到脂酶活性或基因，表明脂酶在该菌的致病机制中不是决定性因子。

One positive clone was obtained from the libraries with the tributyrin flat and ELISA sieve method. Analyzed the sequencing result, the positive clone include the Open Read Frame of low-temperature alkaline lipase gene.

序列测定分析表明，此重组质粒包含有长度为此95lbp的低温脂肪酶基因的完整的开放读码框架和上游调控序列。

The expression of p-p38 and Rho GDIαwas upregulated in LCA by either low shear stress or hypertension. However, the highest expression of p-p38 and Rho GDIαwas observed in LCA of low shear stress accompanied by hypertension.(4) Effect of low shear stress on both decreasing apoptosis and increasing proliferation of VSMCs in LCA was reversed by tributyrin, a HDAC inhibitor.

当高血压伴有低切应力时，两者的协同作用诱导p-Akt的表达水平进一步增加；(3)高血压和低切应力都可以诱导颈总动脉p38和Rho GDIα表达增加；当高血压伴有低切应力时，两者的协同作用诱导颈总动脉p38和Rho GDIα表达进一步增加；(4)HDAC抑制剂Tributyrin可以通过抑制VSMCs增殖，促进VSMCs凋亡，在干预低切应力诱导的血管重建中发挥作用。

To elucidate the possible mechanism of differentiation and /or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor, the level of acetylated histone H3 was detected by Western blot, p21~ WAF1expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin，TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Westernblot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21WAF1表达量的改变。

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin， TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Western blot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21(上标 WAF1)表达量的改变。

A strain NK13 was screened for a certain extent asymmetric hydrolyze the rac-ketoprofen Chloroethyl ester and identified as Bacillus megaterium. For the preparation of gene libraries, a positive clones was obtained from the tributyrin flat. The sequence of this esterase gene had been analysised, and contained the whole ORF of an esterase gene with the length of 933bp.

以本实验室筛选出的一株具有不对称拆分消旋酮基布洛芬氯乙酯的菌株NK13为材料，经初步鉴定为巨大芽孢杆菌，通过构建其基因文库，从中筛选得到一阳性克隆重组子pUC-NK。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。