查询词典 trehalose

The cytoplasmic trehalose concentration in red blood cells, free hemoglobin and ATP level were determined at different incubation temperatures (4, 22 and 37℃), different trehaolse concentrations (0, 200, 400, 600, 800 and 1000 mmol/L ) and different incubation times (2, 4, 6, 8 and 10 hours), the cytoplasmic trehalose, free hemoglobin, hemoglobin and mean corpuscular volume in fresh RBCs and RBCs strored for 72 hours at 4℃ were compared, when loading condition was ensured.

在不同孵育温度（4、22和37℃）、孵育时间（0、2、4、6、8、10小时）、不同负载缓冲液浓度（0、200、400、600、800、1 000 mmol/L）条件下检测新鲜红细胞对海藻糖的成功负载量及红细胞各项理化指标；在固定负载条件下，对新鲜红细胞和4℃保存72小时红细胞海藻糖负载、游离血红蛋白、血红蛋白和红细胞平均体积进行了比较。

The results showed that the mutant contained high trehalose content and high cell survival rate kept even after 4 h 18 %vol alcohol treatment (no respiration deficiency existed), which indicated that high trehalose content could protect cells membrane and prevent DNA loss of mitochondria and exosmosis of intracellular materials.

结果表明，中性海藻糖酶缺失突变株细胞海藻糖含量较高，用18 %vol的酒精处理4 h后仍可保持高的细胞存活率，并且无呼吸缺陷型的出现，说明高海藻糖含量可以保护细胞膜，防止线粒体DNA丢失和胞内物质的外渗，与酵母的酒精耐性之间存在一定的关系。

This thesis primarily optimized the process of trehalose production from Micrococcus (QS412) based on the previous achievements of our research group, and then investigated the feasibility of trehalose production from other bacteriumCellulomonas ...

本文首先在实验室原有成果的基础上，优化了用玫瑰微球菌QS412合成海藻糖的工艺条件，其次对其他菌种生产海藻糖的可行性进行了初步探索。

The survival rates of mice early blastocyst and morula treated with 0.5M trehalose were 61.8% and 95.6% respectively; and treated with 0.25M trehalose were 35.5% and 67.2% respectively.

小鼠桑椹胚和早期囊胚用0.5M海藻糖处理后存活率分别为61.84％和95.57％；用0.25M海藻糖处理后存活率分别为35.50％和67.22％。

Functinal complementation test showed that trehalose-6-phosphate synthase mutant (tps1△), transformed by the open reading frame of SpTPS1 gene, restored growth on the mediam supplemented with glucose as a sole carbon source. This result indicated that trehalose-6-phosphate synthase gene (SpTPS1) of Selaginella pulvinata has biologically activity and is hopful to be applied to transgeneic improvement of plant for abiotic stress tolerance.

酵母功能互补试验证明，用SpTPS1基因开放阅读框转化的海藻糖合成酶基因突变（tps1△）酵母菌株，可恢复在以葡萄糖作为唯一碳源培养基上的生长，说明垫状巻柏海藻糖-6-磷酸合成酶基因SpTPS1具有生物活性，可能应用于植物抗逆性的转基因改良。

The loading process of trehalose has no effect on apoptosis of platelets. The efficacy of trehalose plus phosphate in suppressing apoptosis of platelets during cryopreservation is superior to that of only trehalose. Compared to 6% DMSO and 2% DMSO plus TS, trehalose plus phosphate can retain the same platelet count, superior hypotonic shock response and platelet aggregatory reaction to ADP, and lower apoptosis rate of platelets. Platelets cryopreserved with trehalose plus phosphate can retain intact ultrastructure.

结论血小板负载海藻糖的过程不会促进血小板的凋亡；海藻糖保存血小板时，联合应用磷酸盐在抑制血小板凋亡方面的效果优于单独应用海藻糖；海藻糖联合磷酸盐冻存血小板的长期效果较稳定，其维持血小板回收率的效果与6%DMSO及2% DMSO联合TS相同，维持血小板低渗休克反应、聚集功能及抑制血小板凋亡的效果优于6%DMSO及2%DMSO联合TS，并能较好地维持血小板的超微结构。

The mechanism in which trehalose is produced from dextrin or starch hydrolyzate by endocellular enzymes of bacterium D-97 can be elucidated by high performance liquid chromatography with differential refraction detection basically, including the effect of the different carbon sources on the endocellular trehalose-producing enzymes in bacterium D-97 and the possibility or ability of the endocellular enzymes to produce trehalose using maltooligosaccharides of different chain lengths.

通过高效液相色谱/示差折光检测系统分析可获得细菌D-97利用糊精或淀粉水解物合成海藻糖的基本生物学信息，包括微生物培养碳源对细菌D-97胞内海藻糖合成酶系的影响以及该酶系利用不同种类或不同分子链长度的麦芽寡糖合成海藻糖的能力及作用过程。

By the partial purification of Arthrobacter nicotinovorus D-97 endocellular enzymes and the results of enzymatic reaction of enzymes of partition collection, it was found that enzymes related to trehalose synthesis were consisted of Trehalose-forming Enzyme and Trehalose-releasing Enzyme . Trehalose-forming Enzyme could convert maltooligosaccharides into non-reducing maltooligosyl trehalose by intramolecular transglycosylation, and Trehalose-releasing Enzyme could hydrolyze α-1, 4 linkage between the trehalose residue and the maltooligosaccharide residue to release trehalose and maltooligosaccharides with two glucose monomer lesser.

将食尼古丁节杆菌D-97胞内酶部分纯化并对各收集组分进行酶反应，发现其海藻糖合成酶系由海藻糖生成酶(Trehalose-forming Enzyme，TFE)和海藻糖释放酶(Trehalose-releasingEnzyme，TRE)组成，前者分子内转糖基将直链麦芽寡糖转化为没有还原性的麦芽寡糖基海藻糖，后者专一切割麦芽寡糖基海藻糖分子上麦芽寡糖基与海藻糖连接的α-1，4糖苷键，从而释放出海藻糖和减少了两个葡萄糖单体的麦芽寡糖。

The results showed that the conidia of the RNAi transformant accumulated more trehalose, lost viability more slowly during prolonged storage and showed reduced heat shock sensitivity than the wild type, and the overexpression transformant has reverse results. These results indicate that neutral trehalase in M.anisopliae control trehalose concentration via degradation of trehalose, and indirectly influnce the viability of conidia during prolonged storage and the resistance to heat stress.

以上研究结果表明，金龟子绿僵菌CQMa102孢子体内海藻糖是由中性海藻糖酶降解的，海藻糖含量与耐储性和耐热性具有正相关性，中性海藻糖酶在虫生真菌金龟子绿僵菌孢子中的生理功能是通过降解分子内海藻糖调控海藻糖含量，从而影响其对不良环境的抗性，如储藏性、耐热性。

Importantly, chemical compounds, such as Congo red and trehalose, can ameliorate polyQ-mediated protein aggregation and reduce the symptoms of HD in transgenic mice.

现今有两种化合物—Congo red和trehalose，被发现可以减少polyQ蛋白的聚集，进而减轻HD转殖基因老鼠的病徵。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。