查询词典 trehalase

The result discovered that the trehalase expression of the Metarhizium anisopliae preserved reaches the maximum value the third day to drop afterward with the same trends of un preserved.

结果发现，经过保存的绿僵菌，其海藻糖酶的表达趋势和未保存的基本一致，第三天达最大值随后表达量下降。

Baker's yeast;trehalose;freeze tolerance;trehalase mutant

面包酵母；海藻糖；冷冻耐性；海藻糖酶突变株

In our research, the neutral trehalase gene in M. anisopliae was successfully cloned by PCR, RT-PCR, RACE and panhandle PCR, and submitted to GenBank, accession number are AY557613 and AY557612.

本研究利用PCR、RT-PCR、RACE、panhandle PCR 等方法成功地克隆了金龟子绿僵菌CQMa102 中性海藻糖酶基因，并登录NCBI 的GenBank，登录号为：AY557613， AY557612。

In order to enhance the trehalose production, Y7 was used for mutation with EMS. Through primary screening and compound screening, strain L61 with high-yielding trehalose and deep neutral trehalase activity was screened.

实验对出发菌株Y7进行了EMS诱变处理，通过初筛、复筛，从中选育出一株能高产海藻糖同时具有较低海藻糖分解酶活性的菌株L61。

Keywords: Entomopathogenic fungus; Metarhizium anisopliae; neutral trehalase, storage stability

中文关键词：虫生真菌；金龟子绿僵菌；中性海藻糖酶；储藏稳定性

Trehalase plays an important role in glycogen accumulation in the formarion of diapaused eggs. At the beginning of diapause, glycogen phosphorylase is the key enzyme to glycogen decomposition and the formation of sorbitol and glycerol, whereas at the stage of diapause termination, NAD-sorbitol dehydrogenase is the key enzyme to sorbitol decomposition and glycogen re-synthesis.

在滞育卵形成过程中，海藻糖酶对糖原的积累起着重要的作用；在滞育开始时，糖原的分解与山梨醇、甘油的形成，糖原磷酸化酶是关键酶；在滞育解除时，山梨醇的分解与糖原的再生成时山梨醇脱氢酶(NAD-sorbito1 dehydrogenase)是关键酶。

Sugar and protein content in the haemolymph after fed with transgenic rice Total sugar content in the haemolymph of SSB fed with KMD1 was significantly lower than that of the control, while trehalase activity was significantly higher than that of the control, but there were no significant differences on trehalose concentration and glucose concentration between the treatment and the control. However, there were no significant differences on all the four components mentioned above between SSB fed on MSB and the control.

四、取食转基因抗虫水稻后二化螟幼虫血淋巴糖代谢和蛋白质浓度的变化五龄二化螟幼虫取食KMD1后血淋巴总糖含量显著低于取食秀水11对照的，海藻糖酶活性显著高于对照，而海藻糖含量和葡萄糖含量与对照组相比都无显著差异；取食MSB后二化螟血淋巴总糖含量、海藻糖含量、葡萄糖含量以及海藻糖酶活性与取食明恢86的对照相比，均差异不显著。

The glucose-6-phosphate dehydrogenase increased during overw intering,indicating that reduced condition of cell might keep the cell in pr oper function even in cold temperature,and also for cryoprotectant synthesis.The changes in trehalase activity showed that low activity was for keeping higher tre halose content and high activity for removing it in the spring.

越冬期间，体内葡萄糖-6-磷酸脱氢酶活性增高所产生的还原型烟酰胺腺嘌呤二核苷酸磷酸，可为细胞在亚低温状态下发挥正常功能以及体内抗冻保护性物质的合成提供还原动力，同时通过调节体内海藻糖酶活性来维持越冬期间较高含量的海藻糖和移除春季体内累积的过多的海藻糖。

The results showed that the conidia of the RNAi transformant accumulated more trehalose, lost viability more slowly during prolonged storage and showed reduced heat shock sensitivity than the wild type, and the overexpression transformant has reverse results. These results indicate that neutral trehalase in M.anisopliae control trehalose concentration via degradation of trehalose, and indirectly influnce the viability of conidia during prolonged storage and the resistance to heat stress.

以上研究结果表明，金龟子绿僵菌CQMa102孢子体内海藻糖是由中性海藻糖酶降解的，海藻糖含量与耐储性和耐热性具有正相关性，中性海藻糖酶在虫生真菌金龟子绿僵菌孢子中的生理功能是通过降解分子内海藻糖调控海藻糖含量，从而影响其对不良环境的抗性，如储藏性、耐热性。

By means of microscopic observation, we found the body wall ofchemical-stressed SCN shrinked, and stylets of some stressed SCN breaked through the body obviously. Trehalose content and trehalase activity of stressed and unstressed SCN wereassayed by enzyme-labeled instrument.

利用显微镜对虫体进行观察发现，SCN受到化学胁迫后，体壁发生不同程度的缢缩现象，有的胁迫处理后虫体头部口针明显突出体外。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。