查询词典 treat a person with sincerity

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

If Sequencing Batch Reactor was used directly to treat anaerobically digested effluent, the treatment efficiency was very poor, with only 8.31% for COD removal rate, and 78.7% for NH 3 N.

采用序批式活性污泥法工艺直接处理厌氧消化液，污染物的去除效果很差，COD仅去除8.31%，NH3-N去除7 8.7%。

OBJECTIVE: Clinical effects of TangLuoTong on DPN are observed in order to find a new and effective anagraph to treat on DPN in Chinese medicine.

目的：观察糖络通对糖尿病周围神经病变的临床疗效，从而在中医药中探索出治疗DPN的有效的新方药。

Hair transplants and scalp reduction surgery are available to treat androgenetic alopecia.

Hair和头皮移植手术，可减少治疗雄激素性脱发。

Someone said that the oriental had anepia, and proposed to go back to the ancient time to treat this anepia.

有人说，东方曾经失语，于是提出回到古代去以治疗这种失语。

UCMS cells express growth factors and angiogenic factors, suggesting that they may be used to treat neurodegeneratie disease.

基质和间充质有何区别？

Balloon-expandable stents angioplasty is a safe and effective way to treat insufficient blood supply caused by angiostegnosis, but the long-term curative effect should be observed further.

血管内支架成形术是治疗血管狭窄引起脑供血不足的安全、有效方法，其长期疗效还需进一步随访研究

Balloon-expandable stents angioplasty is a safe and effective way to treat insufficient blood supply caused by angiostegnosis, but the long-term curative effect should be observed further.

血管内支架成形术是治疗血管狭窄引起脑供血不足的安全、有效方法，其长期疗效还需进一步随访研究中国诺宇综合网http：//www.siaaa.com

Balloon-expandable stents angioplasty is a safe and effective way to treat insufficient blood supply caused by angiostegnosis, but the long-term curative effect should be observed further.

血管内支架成形术是治疗血管狭窄引起脑供血不足的安全、有效方法，其长期疗效还需进一步随访研究中国论文联盟http：//www.lwlm.com

To explore the properties of the cortical response to visual coherent motion and reveal the possible neural mechanism of cortical deficit of visual motion perception in anisometropic amblyopia so that we can diagnosis and treat it earlier and specifically.

以期能进一步揭示屈光参差性弱视视觉运动功能缺陷的可能皮层机制，为早期发现和早期治疗潜在的弱视病人提供理论依据并帮助临床根据弱视不同的脑功能区损害特点进行有针对性的诊疗。材料与方法1。

Although translator has turned from being a crystal ball by which the original culture can unrestrainedly penetrate to another crystal ball by which the target culture can freely traverse, the translator's personal embodiment, in the process of cognitive act, are still absent in translation studies. Translators are still subjects without body or simply disembodied subjects.

译者虽然由原语文化可以自由穿透的玻璃球变成了译语文化可以自由穿越的玻璃球，但译者认知过程中的个体体验在翻译研究中依然缺席，译者依然仅仅是一个没有躯体体验的主体。

Chillingly, he claimed our technology is 'not nearly as sophisticated' as theirs and "had they been hostile", he warned 'we would be been gone by now'.

令人毛骨悚然的，他声称我们的技术是'并不那么复杂，像他们一样，和"如果他们敌意"，他警告说，'我们将现在已经过去了。