查询词典 transforming

Objective To observe the expression of bone morphogenic protein-2(BMP-2),transforming growth factor-β1(TGF-β1) and vascular endothelial growth factor in fracture healing after treated with Cervus and cucumis polypeptide injection so as to find out its action mechanism.

目的观察鹿瓜多肽注射液对骨折愈合过程中骨形态发生蛋白2(BMP-2)、转化生长因子(TGF-β1)和血管内皮生长因子等生长因子表达的影响，了解鹿瓜多肽注射液促进骨折愈合的分子生物学机制。

Methods Serum contents of hyaluronic,type IV collagen,cholalic acid,tumor necrosis factor,transforming growth Factor-α,interleukin-1(IL-1),interleukin-6(IL-6)and interleukin-8(IL-8)were detected by radioimmunoassay and were analyzed.

方法应用放射免疫法对患者血清透明质酸、IV型胶原、胆酸、肿瘤坏死因子、转化生长因子-α、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)进行测定和分析。

Methods Serum contents of folic acid, vitamin B12 （VB12 ）, cholalic acid, interleukin（IL-2）, tumor necrosis factor, hyaluronic acid, type IV collagen, transforming growth factor-α, and cortisol were detected by radioimmunoassay and analyzed.

应用放免测定法对患者血清叶酸、VB12、胆酸、白细胞介素素-2（IL-2）、肿瘤坏死因子、透明质酸、Ⅳ型胶原、转化生长因子-α、皮质醇进行测定和分析。

Abtract Objective To study the level of the expression of PTTG mRNA in the cholecystic tissues of gallbladder adena?鄄carcinoma, chronic cholecystitis and gallstone. Methods In situ hybridization was used to detect PTTG mRNA on the routinely paraffin?

目的 研究胆囊腺癌、慢性胆囊炎和胆囊结石病例的胆囊组织中垂体肿瘤转化基因（pituitary tumor transforming gene， PTTG）mRNA表达水平及其临床病理意义。

Key words: umbilical cord blood;mesenchymal stem cells; chondroblast; transforming growth factor-β1

脐带血；间充质干细胞；成软骨细胞；转化生长因子-β1

Aim To observe the effect of transforming growth factor-β on promoting the process of chondrogenesis and osteogenesis under the periosteum of mouse femur.

目的 观察转化生长因子-β在小鼠股骨骨膜下启动骨及软骨生成过程。

Methods Autologous BMMSC of New Zealand rabbits were acquired and induced into chondrogenic differentiation by adding transforming growth factor-β1 (TGF-β1), dexamethasane and ascorbic acid in vitro. Then the chondrocytes were implanted onto novel polymeric scaffolds made by Poly (dl-lactide-co-glycolide) embedding with chitosan nonwoven cloth. The tissue engineering cartilaginous grafts composed of chondrogenetic BMMSC/scaffolds were finally transplanted into the defects of ear cartilage in rabbits of the experimental group.

取新西兰大白兔BMMSC体外培养扩增，以含转化生长因子(TGF-β1)、地塞米松和维生素C的培养液做诱导培养，实验组以诱导后的软骨细胞与聚丙交酯－乙交酯共聚物Poly(dl-lactide-co-glycolide)包埋甲壳胺无纺布的新型支架形成复合物修复兔自体耳廓软骨缺损；对照组以PLGA/甲壳胺无纺布支架修复兔耳廓软骨缺损。

"Our study shows that presering and restoring forests is likely to be climatically ineffectie as an approach to slow global warming," said Ken Caldeira, a co-author of the study from the Carnegie Institution. To preent climate change, we need to transform our energy system. It is only by transforming our energy system and presering natural habitat, such as forests, that we can maintain a healthy enironment.

&我们的研究表明，保存及重建森林好象是很无效的减缓全球变暖的方法&共同研究者，卡内基研究所的Ken Caldeira说，为了阻止气候变化，我们需要改造我们的能源系统，保存自然栖息地，例如森林，我们才能维护健康的环境。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷