查询词典 transformation myth

The results Showed that the highest transformation efficiency was obtained through the following transformation procedure: after preculture for 2 d, the explants were in fected 3 min with 50 mg/L acetosyringone and OD600=0.5 Agrobacterium culture liquid, and then co-cultivated for 2 d. Resistant shoots from leaf disks were screened on MS0.4 mg/L NAA + 100 mg/L Cef medium supplemented with 10 mg/L Km.

结果表明：2 d的预培养，OD600值约为0.5的农杆菌菌液添加50 mg/L AS，侵染时间3 min，2 d的共培养，从分化培养基获得的抗性芽在生根培养基MS+0.4 mg/L NAA+10 mg/L Km+100 mg/L Cef上进行延迟筛选，有利于获得较高的转化效率。

Factors that influence percentage of transformation including time of preculture, dipping and coculture, type and content of antibiotic, day of delayed selection culture, intension of selection were studied. The effective transformation system of "Starkrimson" was developed as follow: Leaves precultured in darkness for three days were dipped into agrobacterium suspension for three minutes, then were cocultured in darkness on MS medium with TDZ0.5mg/L and NAA 0.3mg/L for three days, and then cultured in darkness on MS medium with TDZ0.5mg/L NAA0.3mg/L and Cef 250mg/L.

通过对叶片预培养时间、侵染时间、共培养时间、抑菌素种类和浓度、延时筛选时间、不同选择压等遗传转化影响因素的研究，建立的'新红星'苹果遗传转化体系为：叶片黑暗预培养3天，经农杆菌侵染3分钟，在含TDZ0.5mg/L，NAA0.3mg/L的MS培养基上黑暗共培养3天，然后加250mg/L Cef黑暗维持培养3天，再进行Km12.5mg/L的选择压黑暗培养至抗性愈伤出现；转为光照条件1-2次的继代筛选，获得抗性芽后，进行扩繁。

The results showed that: the explants of strawberry leaves were rather sensitive to kanamycin, and the corresponding concentration of 20 mg/L could substantially inhibit the growth of non-transformed tisse and be used to screen the leaves of strawberry after transformation. Timentin at the concentration of 300 mg/L could be used to eliminate Agrobacterium tumefaciens and had minor impact on the growth of the explants. The preculture time of 2-4 days was beneficial to improve transformation frequency. Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium. The bacterial concentration of OD(subscript 600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明：草毒叶片外植体对卡那霉素较为敏感，适宜的筛选浓度为20mg/L，可明显抑制非转化组织的生长；300mg/L的羧吩青霉素可有效抑菌，对外植体生长影响也较小；预培养2-4d有利于转化；共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长；OD(下标 600nm)值为0.4的菌液侵染10min效果最佳。

GCJ8 activate medium, preculture 2 days, 5 X Ca concentration in preculture medium and coculture medium, inoculation I mineture,4mg li1 AgN0 and 0.7% P\P, delay selection 5 days will obtain the highest transformation frequenc Using 'pingyitiancha'shoot apex, mediated by A.tum as above system, the HVAIgene, was transformed into pingyitiancha and obtained Kam resistance rerzeneration plants, it is confirmed that 6 plants of them is transformation plants by identified by PCR and dot blot

按照上述体系，以平邑甜茶茎尖分生组织为受体，将HVA1基因导入平邑甜茶中，获得了具有Kam抗性的再生植株，经PCR与点杂交鉴定，证实有6株为转化植株。

Phase transformation and incomplete phase transformation behaviors of TiNi shape memory alloy within small prestrain level (＜10％) were studied by DSC and recovery strain measurements.

采用DSC方法和回复应变的测量，对小预应变（＜10％）TiNi形状记忆合金的相变及不完全相变行为进行了研究。

This thesis holds that all of Chao Yu's dramas should be divided into former period and latter period; the deep structure of former Chao Yu's dramas is the contrast and opposition of an ideal world to a prisonlike world, and the deep structure of latter Chao Yu's dramas is the"clear sky"; the transformation of former Chao Yu's drama to the latter is a transformation of its deep structure.

本文认为曹禺戏剧存在着前期和后期之分；前期曹禺戏剧的深层结构是闭锁的世界与理想国的对照与对立，后期曹禺戏剧的深层结构则是&明朗的天&；从前期戏剧到后期戏剧的转换是深层结构的转换。

And sometimes even purposedly repressed. Therefore, in this period Xinmin was more expressed in the nature of nationalism than the modern nature of individulism of nationality transformation.From Xinhe Revolution (1911) to the eve of MayFourth Movement, with the mitigation of national crisis, rapid development of the country's capital economy and intelligentsia's deeper understanding of western culture, thought about nationality transformation developed into a brandnew stage -that of Liren.

这一时期的国民性改造思想在个人与国家、民族的关系上，实现了手段与目的的根本转换，人自身，即人的生命、尊严、价值、需求成为资产阶级思想家关注的重心，他们呼吁突显&自我&，在价值观上要求以资本主义的个人本位主义取代封建的家族本位主义，在道德观念上以独立、自由、平等的资产阶级近代规范取代三纲五常的封建道德规范

In the recent years, with the confirmation of the reformational target of the market economy system and the quickening of the transformation of the social structure, it has become an urgent requirement for the government to reform the old pattern of social security system so that it may be cooperative with the restructuring of the economic system and the transformation of social structure.

近年来，伴随着建立社会主义市场经济体制的改革目标的确定和社会结构转型的加快，改革计划经济体制下形成的旧的社会保障模式，使之与我国经济体制改革与社会结构转型相配套，已成为迫切需要解决的问题，亦成为学术探讨的一个热点。

Aimed at Wigner-Ville transformation crossterms badly interfering signals' resolution in the time-frequency analysis, the improved smoothed Wigner-Ville transformation is employed to analyze the time-|frequency characteristics of the Ricker wavelet synthesis record.

针对 Wigner-Ville变换中交叉项对信号时频分析的分辨率有较大的干扰，采用改进了的平滑Wigner-Ville变换处理技术，来分析雷克子波合成记录的时频特征，能够提高时频分析在时间和频率上的分辨率。

Aiming at local hydrological feature, the problem of transport and transformation of heavy metal in river network regions through mathematical modeling at the first time. Establishing modeling of heavy metal transport and transformation, dissolved heavy metal was the main research goal and the synthetically sedimentation coefficient was imported to investigate the migration of heavy metal between aquatic phase and sedimental phase.

针对区域水文特点，首次采用数学模拟的方式对平原河网地区的重金属迁移转化问题进行研究；建立重金属迁移转化模型，以溶解态重金属为主要研究对象，通过引入综合沉降系数分析重金属水相和沉积相之间的相互迁移过程。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。