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Thy-1.1~+ OT-I CD8 T cells were adoptively transferred into Thy-1.2~+ host C57 mice treated with either isotype or PK136 Ab as mentioned above followed by injection i.v.withΔactA LM-OVA.On day 7 after infection,equal number of Thy-1.1~+ CD8 T effector cells from either hosts were sorted and adoptively transferred into second host mice(Thy-1.2) which were also on day 7 after infection and treated with isotype or PK136 Ab,respectively.

为进一步阐明NK细胞促进CD8Tm细胞的产生是作用于收缩期而不是扩增期,我们设计了如下的实验:将Thy-1.1~+ OT-I CD8 T细胞过继回输到经PK136抗体或对照抗体处理的C57小鼠体内并用ΔactA LM-OVA感染,7天后将两组小鼠脾脏中的Thy-1.1~+ CD8T细胞分离纯化后等量分别回输到同步感染的经PK136抗体或对照抗体处理的第二受体小鼠体内。

Then the fiber was transferred to a 4 mL vial which was filled with 100 μL of trifluoroacetic anhydride and stood 30 sec for headspace extraction. Afterward the fiber was transferred again to another blank 4 mL vial and stood 10 min for on-fiber derivatization before the analysis with GC/MS. The absorption-time profiles for both furoic acid and creatinine were examined.

有关尿中肌酸酐的量测,首先亦使用65μm PDMS/DVB纤维,在电磁搅拌转速1200rpm下以直接样本接触方式吸附60秒后,将已吸附肌酸酐的纤维挪至装有100μL无水三氟醋酸的4mL样本瓶中顶空吸附30 sec,再将纤维挪至另一个空白的样本瓶并静置10min(以利衍生物trifluoroacetamide的生成),最后再以气相层析质谱仪进行分析。

However in Hachijo population, nymphal development was the fastest under LD 12:12h, followed by LD 14:10h and the slowest under LD 16:8h. Nymphal growth is restrained when the nymphs are transferred from LD 16:8h to LD 12:12h at 60 and 120 days after hatching, rapid development is observed when nymphs were transferred in the opposite direction in Hachijo population.

八丈岛种群的若虫在短日条件下发育最快,其次是中间日长,长日条件下若虫发育最慢,孵化后60d或120d将若虫自LD 16:8h向LD 12:12h转移抑制若虫发育,反方向转移的若虫快速发育。

There are two defferent design of hardware: optical fiber temperature sensor is used for middle-voltage Metal-clad switchgear, which data is transferred by optical fiber; another one is 1-Wire Digital Thermometer, it is used for lower-voltage Metal-clad switchgear, and the data is transferred by unwired.

硬件方面设计了两种不同的温度传感系统:中压开关柜过热故障监测采用光纤式温度传感器,同时采用光纤传输数据;低压开关柜采用单总线数字式温度传感器,数据采用无线传输。

When the case is transferred for examination before prosecution, the list of the documents on the distrained articles, the certificates on the frozen deposits and other materials shall be transferred along with the case.

在移送审查起诉时应当将扣押物品文件清单、冻结存款证明文件等材料随案移送

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上,并以脂质体介导的方法将重组载体转染包装细胞PA317,以G418筛选克隆细胞,浓缩克隆细胞上清以制备重组病毒液,继之感染NIH3T3细胞后,进行Southern blot和Northern blot分析,检测目的基因在靶细胞的整合与转录水平。

The results showed that the N distribution centre changed with the shift of the growth centre.N mainly distributed in leaf and culm before silking stage,then the N distribution centre transferred to ear after silking stage,and then transferred to kernel formation gradually.

结果表明,糯玉米生育期间氮素的分配中心是随生长中心转移而变化的,开花前主要分配在叶片和茎秆中,开花后氮素的分配中心开始由茎、叶转向雌穗,并逐渐以子粒建成为中心。

When leased land is subleased, the right and obligations defined in the lease contract and the register document are thereby transferred, and the property rights to buildings and other attached installations on the ground are also transferred.

第十九条土地使用权转葼时,出让合同和登记文件中所载明的权利、义务随之转移,地上建筑物、其他附着物的所有权也随之转让。

When examining the relative positions of the valence band and the conduction bands in Table 2, it is evident that it is in fact thermodynamically feasible for the generated electrons in titanium dioxide to be transferred to the lower lying conduction band of any the iron oxides phases present, while the photogenerated holes may also be transferred to the upper lying valence bands of the iron oxides.

当检查原子价的比较位置的时候表 2 的乐团和传导乐团,它是显然的那它事实上热力学能实行对钛二氧化物的被产生的电子被转移对比较低的横躺传导乐团任何的那氧化铁时期呈现,当 photogenerated的时候洞也可能被转移到上面的横躺原子价氧化铁的乐团。

Automatic color transfer effectively solves this issue. Carve up Roseau and subtractive clustering is applied in this paper. Completely automatic algorithm implementation is realized. In clustering, pixels are selected according to their brightness, and those with larger data density are cluster to sample block. First of colors in the same block are transferred first, others are transferred later.

本文将网格划分减法聚类应用到颜色迁移中,完全实现算法的自动化,聚类方法按照亮度值的大小依次聚类,从各个聚类域中提取出数据点密度较大的像素点集组成样本块,首先对每个样本块间进行颜色迁移,再完成样本块以外的其他像素的颜色迁移。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。