查询词典 transcriptional

MicroRNAs are a class of small regulatory RNAs, which act as post-transcriptional modulators of gene expression.

中文摘要 MicroRNA 是一种有调节能力的小分子的RNA，作为基因转录后的调控者。

EF116490. Characterization and comparison of this sequence with mouflons, goat, cattle and European bison orthologues were also conducted. Results showed that: 1 The 5'-flanking region contained many potential transcriptional factor binding sites such as those for C/EBPb, C/EBP, SP1, Cap, USF, HFH-2, HNF-3b, and Oct-1, which might have an important effect on transcription activation and regulation as well as tissue-specific expression.

EF116490，在分析其序列结构特征的基础上与GenBank中摩弗伦羊、山羊、普通牛、欧洲野牛进行了比较基因组学和系统进化研究，结果表明：(1)欧拉型藏绵羊GHR基因启动子P1区存在C/EBP、C/EBPb、SP1、Cap、USF、HFH-2、HNF-3b、Oct-1等多个潜在的转录因子结合位点，可能与GHR基因的转录调控和起始以及特异表达有关。

Using semi-quantitative PCR within the exponential phase, with beta-actin as an external control, the constitutive transcriptional expression of genes of phase I (CYP1A), phase II xenobiotic metabolizing enzymes, HSP70 and ATP1A1 were also investigated.

应用半定量RT-PCR的方法，以β-肌动蛋白作为外参照，在指数期增长范围内分别得到了CYP1A、GSTA、GSTR、HSP70和ATP1A1 mRNA与β-肌动蛋白mRNA的比值，确定黄斑篮子鱼肝脏去毒相关基因的组成型表达水平。

In recent years, some derived yeast two-hybrid systems have been established, including yeast two-hybrid dual bait system, reverse two-hybrid system, membrane-associated two-hybrid system (SRS, TOPIS and split-ubiquitin base two-hybrid system) and transcriptional factors-associated two-hybrid system(RTA system and PolⅢ based two-hybrid system), which promise to overcome some limits and improved the sensitivities of the original yeast two-hybrid system.

十余年来，随着酵母双杂交迅速推广，不断涌现出一些衍生系统，其中包括酵母双杂交的二元诱饵系统，逆向双杂交系统，非转录读出特点的双杂交系统（如Sos蛋白招募系统、PI3K介导的靶蛋白识别系统和断裂-泛素为基础的双杂交系统）以及转录激活因子与其相关蛋白之间的相互作用的双杂交系统（如以polⅢ为基础的杂交系统和RTA系统）等。

As transcriptional factors, no synergized activation of the two pathways was detected.

STAT3和β-catenin都是转录激活因子，但我们在研究STAT3对β-catenin转录活性的影响中尚未发现可喜的结果。

The protein interaction domains was delineated at the N-terminal 50-amino-acid fragment of HCV core protein and the C-terminus of p53. Confocal analysis also revealed that these two proteins colocalize in subnuclear granules and peri-nuclear region. Transfection experiments using a p53-responsive reporter plasmid in HuH-7, Hep3B, HepG2 and H1299 demostrated that full-length HCV core protein could elicit a positive or negative effect on the p53-mediated transcriptional activation depending on the concentration of the HCV core protein.

更且利用p53蛋白C端删除55-95个胺基酸之质体与含p53蛋白结合区之报导基因和表现全长HCV核心蛋白三者之质体於H1299细胞株进行共同转染时，与全长p53蛋白比较，发现HCV核心从增强全长p53蛋白之转活化能力转为抑制p53蛋白缺乏C端55-95个胺基酸之转活化能力，因实验室已有结果证实活体外及活体内缺乏C端55个及75个胺基酸之p53蛋白无法与HCV核心蛋白进行结合(Kao， unpublished data)。

Under the guidance of the concept, we identified the different expression genesbetween the low malignant BTCC and normal transitional epithelium using lasercapture microdissection and gene chip analysis, indicting that high expression ofEZH2, a member of transcriptional repressors, is involved in early development ofBTCC.

在这一概念的指导下，我们利用显微切割和基因芯片技术研究了低恶性膀胱移行细胞癌和正常膀胱黏膜基因表达谱变化，发现在低恶性膀胱癌中转录抑制因子EZH2基因表达增加，提示EZH2基因与膀胱癌的早期形成有关。

And ZNF397nf function as transcription repressors. The different transcriptional regulation activity of the nf isoforms and their interaction with the fu isoforms would contribute to the complexity of the transcription regulation. To further examine the biological function of interaction between different isoforms we also analyze the potential role of ZNF191 and ZNF434 on signaling pathway. We found that ZNF191fu can significantly enhance the HSE-luciferase activity, while ZNF434fu can enhance the AP1-luciferase activity, then we analyze whether the nf isoforms have impact on the fu isoforms.

而这些成员的nf剪接本与它们对应的fu剪接本的转录调控功能有所不同，其中ZNF191nf与ZNF434nf剪接本的转录调控活性相对它们的fu剪接本显著降低，呈现较弱的转录抑制作用，但ZNF447nf剪接本却呈现出显著的转录激活作用，同时ZNF397nf剪接本则表现出显著的转录抑制作用，ZNF396nf剪接本的转录调控作用与其fu剪接本相当。nf剪接本这种与fu剪接本不尽相同的转录调控活性以及能与fu剪接本互作的能力，大大增加了转录调控作用的复杂性，这使得通过少量的转录因子作用来实现众多基因时空表达的不同成为可能。

In mammals the Notch gene family comprise four related genes, Notch1-4, as the Notch ligands that encode transmembrane proteins. Ligand-receptor interaction mediated Notch activation ultimately leads to the conversion of effectors-CSL CBF1, Su(H, Lag-1 proteins, from repressors to transcriptional activators, and subsequent up-regulation of downstream targets.

在哺乳动物中，Notch基因家族包含了4个相关的基因，分别为Notch-1至-4，和Notch受质蛋白一样能被转译成膜蛋白；受质-受体间相互作用所引起Notch受体蛋白的活化会促使作用蛋白-CSL CBF1， Su(H， Lag-1 蛋白扮演抑制分子或者是转录活化分子上的角色改变，并且进一步地正向调节下游的标的基因。

Five expressed genes contributed to the flowering process and embryonic development such as prolamine, gene for allergenic protein, chalcone synthase, putative peptide methionine sulfoxide reductase and acyl carrier protein Ⅱ gene were chosen to hybridize with a 1510-RNA-blot (time-point phenotypes) array. The 1510 RNA blots were prepared into an RNA array for the purpose of conferring and validating global transcriptional profiles on booting, flowering and filling quantitatively and qualitatively at a statistic level. The results verified: Prolamine and allergenic protein were high-expressed in filling grains after being regulated by development, Chalcone synthase and methionine sulfoxide reductase were high-expressed in the leavies induced by light, in the root stressed for nitrogen deficiency.

为了全局性分析基因表达谱以及进一步验证它们与开花过程的相关性，选取参与水稻开花过程和胚胎发育过程的基因：查尔酮合酶、酰基载体蛋白Ⅱ、醇溶蛋白、S-腺苷基甲硫氨酸还原酶、过敏反应蛋白等为探针，与1500个水稻RNA斑点阵列进行杂交时，结果证实：醇溶蛋白和过敏反应蛋白受发育调节在乳熟成穗中高表达，查尔酮合酶和S-腺苷基甲硫氨酸还原酶在叶片中受光诱导、在根中受缺氮胁迫高表达。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐