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Reverse transcription PCR was conducted and cDNA partial sequences of core SNAREs proteins SNAP-25, VAMP2 and Syntaxinl were successfully amplified from Japanese sea perch.

利用核酸序列数据库中SNARE蛋白相关基因的保守序列，设计出基因特异性引物，从鲈鱼脑组织总RNA中逆转录扩增出SNARE蛋白家族核心成员SNAP-25，VAMP2和Syntaxinl的部分cDNA序列。

Results As shown in Western blot analysis, the presence of microsomes did not alter the molecular weight of TM-TNF , but it did result in the cleavage of the IL-2 signal peptide from S-TNFm,suggesting that the leader sequence of TNF might differ from the signal peptide of typical secretory protein in that it seemed not to have undergone cleavage during translation in the rough-faced endoplasmic reticulum. Further enzymatic analysis revealed that TM-TNF was converted into S-TNF through the effect of certain metalloproteinase. Conclusions These results suggest that the mechanisms of TNF-α production may be as follows: Activation of TNF-α producing cells by LPS leads to augmented transcription/translation of TNF-α gene, resulting firstly

实验结果提示17×103 S-TNF产生机制可能是：TNF产生细胞经LPS等激活后，导致TNF基因转录翻译增加，首先形成26×103 TNF，并借助其信号肽疏水氨基酸部分将之&锚定&在细胞膜，成为跨膜型TNF，介导细胞与细胞之间的生物学效应；在某些蛋白酶作用下，可将mTNF的&信号肽&切除，产生17×103分泌型TNF，释放至体液中，在局部或全身发挥作用。

Objective To investigate the promoter methylation status and the transcription level of BLU in bladder carcinoma and their significance.

目的 研究膀胱癌中BLU基因启动子甲基化状态、转录水平及其意义。

A transcription factor GhDREB for regulating the stress resistance of upland cotton and its coding gene are disclosed.

本发明公开了一种调控陆地棉抗逆性的转录因子及其编码基因与应用。

The final product of transcription is then spliced and modified into one of three RNA molecules: messenger RNA, transfer, or ribosomal.

最后产品的转录然后剪接和修改分为三种 RNA 分子：信使 RNA

Flow cytometry techniques was used to to determine cell apoptosis under the action of mifepristone. And expression of survivin gene was evaluated by reverse transcription polymerase chain reaction. Result: Four different condensations dose-dependently inhibited the proliferation of HO8910 cells.the inhibition by mifepristone plateaued at 20 y.

将HO8910细胞进行体外培养，采用MTT法测定，观察不同浓度米非司酮(20，10，5，2.5μmol／L)对HO8910细胞的杀伤活性；通过流式细胞仪分析用药后细胞凋亡的情况；逆转录PCR法检测了米非司酮培养前后Survivin扣只人A的表达，并将结果进行了相关分析。

After 5 days of coculture, the percentage of CD4 CD25 T cells was detected by flow cytometry. T cell proliferation was assessed by ［3H］ thymidine incorporation using a liquid scintillation counter. The expression of Foxp3 in CD4 CD25 T cells was detected by reverse transcription polymerase chain reaction.

众多学者对其可能的作用机制进行了研究，目前认为可能通过与免疫细胞（如T细胞，树突状细胞，B细胞等）直接接触［3］或通过改变细胞因子分泌网络间接影响免疫细胞发挥效应有关，但确切机制仍不清楚。

Electron micrograph showing transcription of ribosomal RNA genes in the developing egg cell of the spotted newt.

电子显微镜显示了斑点蝾螈卵细胞在发育过程中核糖体RNA基因的转录。

During germline transposition, PB could excise precisely from original insertion sites and transpose into the mouse genome at diverse locations, pref erably transcription units.

PB因子在生殖细胞中转座时能从原始插入位点精确切离，并转座到小鼠基因组的不同位点，尤其是基因区。

Interestingly, HG-induced Janus kinase 2 (JAK2)-signal transducers and activators of transcription 1 (STAT1) activation but not STAT3 or STAT5 activation at 30 min were blocked by NO donors and PKG activator.

有趣的是在刺激30分钟后，高糖会明显诱发JAK2与STAT1的活化，但STAT3与STAT5的活化则不受影响。在加入NO供给者及PKG活化剂后均可有效抑制上述高糖的效应。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐