查询词典 transcription

Transfect BHK-21 cells with screened positive recombinants and determine the expression of GFP and transcription level of 2B gene.

将阳性重组质粒转染BHK-21细胞，检测绿色荧光蛋白的表达和2B基因转录水平。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

Our group is interested in plant secondary metabolism and cotton biology. Current research projects include characterization of transcription factors and small RNAs involved in controlling cotton fiber and Arabidopsis development; investigation of terpenoid biosynthesis, particularly in Artemisia annua; and genetic engineering of cotton fiber development, plant resistance to insects, and artemisinin production.

利用遗传学、发育生物学、生物化学和分子生物学等手段克隆和鉴定参与叶形态建成的重要基因，解析它们之间的互作关系，了解叶形态建成的基因调控网络，认识叶形态建成的分子调控机理，为创制具有理想叶片形态的作物遗传改良品种提供理论基础和技术支撑。

Consequences of the rotation of DNA are illustrated in Figure 9.17 in the twin domain model for transcription. As RNA polymerase pushes forward along the double helix, it generates positive supercoils ahead and leaves negative supercoils behind. For each

超螺旋与转录还有更多的关系，RNA聚合酶转录DNA时，解链和复链同时也发生，如同Fig113中所示，这需要整个转录复合体绕DNA旋转或者DNA自己绕它的双链轴旋转，DNA旋转的后果如Fig11.19所示，图中是一个转录的双结构域模型，当RNA聚合酶沿DNA前移时，在酶前产生正双螺旋，酶后为负的双螺旋；每一个双螺旋转角被RNA跨越后都会在前面产生+1转角，后面产生-1转角。

The nucleolus is the site of ribosomal RNA transcription and assembly of ribosomal subunits.This subnuclear compartment forms around an array of repeated rDNA genes.

核仁是真核生物核糖体RNA合成、加工和核糖体亚单位装配的场所，这一亚核空间在成簇排列的串联重复rDNA基因周围形成。

Reverse Transcription polymerase chain reaction with RNA PCR Kit,the product of RT-PCR was determined by electrophoresis of agarose gel (100v, 30s ),the result was shoot by GDS gel imaging system, the staps were scaned and quantitated by computer analysis system.

用RNA PCR试剂盒进行逆转录聚合酶链式反应，RT-PCR产物在1.2%琼脂糖凝胶上电泳(100V，30 min)，用GDS凝胶成像系统拍摄电泳结果，各带用计算机图像分析系统扫描定量。

In mammals, the Notch signaling pathway is critical for embryonic development, as targeted disruption of Notch1 or its downstream transcription factor RBP-J in mice resulted in disorganization of somites and delayed closure of neurotube, which finally leads to early embryonic lethality.

Notch信号途径在哺乳动物的胚胎发育中起着关键的作用。

After transcription of ribozymes and substrate by 32 P, cleavage reaction in vitro was detected.

结果 pM1 GS 716和 pM 1 GS 337在 37℃均有切割活性，pM 1 GS 716的切割效率为 93%，pM1 GS 337为 4 2 %，而 pM1 GS 2 35几无切割效应。

Regulatory DNA elements that bind to repressors and cause repression of transcription.

结合到阻遏蛋白上引起转录阻遏的具有调控作用的DNA元件。

Silencers Regulatory DNA elements that bind to repressors and cause repression of transcription.

沉默子 结合到阻遏蛋白上引起转录阻遏的具有调控作用的 DNA 元件。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。