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Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

SUC2 mainly encodes sucrose invertase. The invertase could hydrolyse sucrose into glucose and fructose which are carbon source for yeast growth. When sucrose is used as the main carbon source, invertase is necessary for the growth of yeast. Accordingly, it is of great significance for yeast glycometabolism to study the sequence, transcription and regulation of sucrose invertase.

蔗糖转换酶基因(SUC2)编码蔗糖转换酶，通过降解酵母细胞外的蔗糖成果糖和葡萄糖来提供酵母生长所需的碳源，在以蔗糖为主要碳源的培养条件下，蔗糖转换酶对于酵母生长必不可少，因此研究蔗糖转换酶基因序列、转录、调控等对酵母蔗糖代谢具有重要意义。

Systemic levels of IL-6, IL-8 and TNF- a increase in patients with acute pancreatitis and correlate with the severity of the disease.A key regulator of cytokine induction is the pleiotropic transcription factor nuclear factor- K B.

核因子-κB(nuclear factor-kappa B，NF-κB)是由Rel蛋白家族成员形成的同源或异源二聚体，它几乎存在所有的细胞，不同的二聚体与不同的DNA序列结合，亲和力和转录激活能力是不一样的。

Among the most celebrated works of this school may be cited: the "Book of Kells", the transcription of which is ascribed to St. Columba, but which in reality belongs to the seventh century; the "Evangeliarum of Durham", belonging to the Diocese of Lindisfarne (British Museum, Cotton manuscripts, Nero D. IV), copied in honour of St. Cuthbert by Bishop Eadfrith (698-721), bound by Bishop thilwald, and ornamented with precious stones by the monk Billfrith, is also of great value.

其中最有名的作品，这所学校可引：&本书的爱尔兰&，转录的，是得益于圣哥伦布，但在现实中属于第七世纪&的evangeliarum达勒姆商学院&，属于教区林迪斯（大英博物馆，棉花手稿， Nero的四四），复制的荣誉，止于圣主教eadfrith （ 698-721 ），受主教thilwald ，装饰与宝石所和尚billfrith ，也具有极大的价值。

Breast tissues were taken at surgery from patients with breast adenoma, nodule of lobuli glandulae mammariae and breast cancer. Normal samples adjacent to breast cancer were used as control. NIS mRNA and protein were assessed in these samples by reverse transcription polymerase chain reaction , Western blot and immunohistochemistry methods.

1。乳腺组织取自乳腺纤维瘤、乳腺小叶增生以及乳腺癌患者，另取癌旁正常乳腺组织作为对照，分别运用半定量逆转录聚合酶链反应、Western Blot方法和免疫组织化学技术检测NIS的基因和蛋白表达情况。

However, we compare the alignment of deduced ammo acid sequences between DMO cDNA from Oreochromis aurea and DMRT1 (DM-related transcription factor 1 )cDNA from Oreochromis niloticus, fugu, rainbow trout, medaka, rat and human.

而与尼罗罗非鱼，红鳍东方豚，虹鳟，青鲻，鼠，人等动物的DMRT1进行比较，同源性分别为：25.7％，25.8％，24.3％，29.7％，22.5％，22.0％，这么低的同源性说明DMO和DMRT1可能起源于不同的基因。

Observe the change cardiac rhythm and rate;The expression of GimRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针异搏定所致的缓慢性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Observe the change cardiac rhythm and rate;The expression of Gi-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Methods: Arrhythmia was induced with cordilox, and acupuncture two-aide "Neiguan" by electro-acupuncture later. Observe the change cardiac rhythm and rate; The expression of G mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针异搏定所致的缓慢性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白 mRNA的相对含量。

Methods: tachyarrhythmia was induced with aconitine, and acupuncture two-side "Neiguan" by electro-acupuncture later. Observe the change cardiac rhythm and rate; The expression of G-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。