查询词典 transcription

Auxin response factor is an important transcription factor for auxin signal transduction pathway. In order to identify the function of cotton auxin response factor GhARF3, some bioinformatics methods were used to analyze GhARF3 gene sequence, including BLAST analysis, domain analysis, phylogenetic tree analysis, the physical and chemical properties analysis of protein, hydrophobicity analysis, disulfide bonds analysis, phosphorylation site analysis, secondary structure and tertiary structure prediction, and subcellular localization and protein functional classification analysis.

运用生物信息学方法对棉花GhARF3基因序列进行BLAST分析、结构域分析、进化树分析、编码蛋白质的理化性质分析、疏水性分析、二硫键分析、磷酸化位点分析、二级结构以及三级结构的预测、亚细胞定位和蛋白质功能分类预测等分析。

Methods: Four hantavirus strains, which were positive by antigen immunofluorescence assay, were analyzed by reverse transcription polymerase chain reaction, nucleotide sequence and cell culture.

采用逆转录－聚合酶链反应、核苷酸序列测定和细胞培养方法对免疫荧光抗原阳性的4份鼠肺标本进行汉坦病毒分析。

One fragment of 248 bp cDNA of the GABAa recep tor gene from the fourth instar larvae of diamondback moth was amplified, cloned, sequenced and analyzed by using reverse transcription polymerase chain reaction method.

利用反转录多聚酶链式反应对小菜蛾的γ-氨基丁酸a 受体基因cDNA片段进行了克隆和序列分析。

Cerevisiae have found that bZIP proteins play a role in yeast anti-oxidation. at3g51960, the transcription factor found in plants, is highly homologous to that of yeast. To examine the in vivo role of at3g51960, the overexpression and silencing vectors were constructed respectively. By using flower dip-dye, the binary expression vectors, pCAMBTA3301: at3g51960 and pCAMBTA3301: at3g51960Promoter, as well as vectors pCAMBTA3301: at3g51960-GFP and pCAMBTA3301: at3g51960cDNA were transformed to Arabidopsis by Tumefaciens. The transgenetic homozygous Arabidopsis strains have been derived after screening. Analysis on these strains reveals that strains overexpressing at3g51960 gene have higher level of stress resistance, implying a role of at3g51960 in plant stress regulation.

酵母中发现了bZIP家族的转录因子与酵母的抗氧化性相关，植物中的转录因子at3g51960与酵母中的这一转录因子在空间结构上有很高的同源性，为了验证它在体内的功能，本实验构建了过量表达载体和沉默载体，利用花浸染法把农杆菌携带的双元表达载体，pCAMBTA3301： at3g51960，pCAMBTA3301： at3g51960 Promoter和pCAMBTA3301： at3g51960-GFP及pCAMBTA3301： at3g51960 cDNA转入拟南芥，通过多次筛选已经获得了转基因拟南芥的纯合植株，对获得的转基因植株的表型分析发现，在过量表达at3g51960的转基因拟南芥中对胁迫的抗性有一定程度的变化，因此推测at3g51960可能参与植物的抗性调节。

With the methods of Reverse transcription-PCR and cDNA sequencing, the expression of FHIT gene was detected in radiation carcinogenesis. The results show that: 1, The FHIT gene's sequence in normal BALB/c mice compare with the sequence of mice in Ge neBank which absents exon 3, but the function of FHIT protien doesn't alter.

结果发现：1、正常BALB／c 小鼠的FHIT基因序列同GeneBank中小鼠的FHIT基因序列比较缺少了外显子 3，但其表达的蛋白功能并未发生改变；2、FHIT基因在辐射损伤与辐射致癌的早期过程中，对照组血液、胸腺及骨髓均未出现异常的FHIT转录本的表达，不同剂量照射组中血液、胸腺及骨髓均有部分样品出现分子量较小的异常FHIT 转录本的表达。

Objective To study the effect of signal transducer and activators of transcription 5(STAT5)on airway inflammation of rats with asthma.

目的探讨信号转导子和转录激活子5(STAT5)在哮喘气道炎症中的作用。

Activators Proteins that increase transcription of a gene.

激活蛋白促进基因转录的蛋白质。

The expression of cytochrome P450 3A (CYP3A) subfamily implicated in drug metabolism in human fetal adrenals and livers was detected by using enzyme assays and the reverse transcription polymerase chain reaction.

采用酶学和逆转录聚合酶链式反应技术，研究与药物代谢密切相关的细胞色素P450 3A( CYP3A)在胎肾上腺和胎肝中的表达。

Some evidences showed that GA had effects on the post-transcription level of α-amylases.

有证据表明GA在α-淀粉酶的转录后水平的调节上也有作用。

To understand the listed contents:the studies about component of nuclear pore complex; the main functions of gp210;the different configuration models of sequence specific DNA-binding proteins; karyotype and chromosome bandings; configuration of chromatin and dene transcription; nuclear bodies is many subnuclear domains which have different conformation between chromatin.

了解核孔复合体成分的研究；gp210主要的功能；序列特异性DNA结合蛋白的不同结构模式；核型与染色体显带；染色质结构与基因转录；染色质之间的空间发现的许多形态上不同的亚核结构域——核体。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。