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To get more informationon gene regulation during erythroid differentiation of K562 cells, we applied modifieddifferential display reverse transcription polymerase chain reaction methodto identify genes showing differential expression in uninduced and hemin-induced K562cells.

为进一步获得分化过程中相关基因表达的信息，我们应用改良的差异显示RT-PCR技术，分析K562细胞在Hemin诱导的红系分化前后的基因表达变化，共获得357条差异表达带。

In uninduced HEL cells, GATA-2 transcription factor can specifically bind to the regulatory elements of human β-globin gene, including the proximal regulatory element (theβ-promoter) and the distal regulatory elements (the DNaseⅠ hypersensitive sites in the locus control region, HS2-HS4 core sequences).

在非诱导的HEL细胞的核蛋白中，能够与β—珠蛋白基因的近端启动子调控序列及远侧端LCR调控序列（HS2，HS3和HS4核心DNA序列）相结合的蛋白质主要是GATA—2转录因子，而在羟基脲诱导的HEL细胞核蛋白中，与上述调控序列相结合的蛋白质主要是GATA—1转录因子。

HTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start site.

人端粒酶逆转录酶是一种核糖核蛋白酶，由端粒RNA和端粒酶蛋白组成的一种特殊逆转录酶，在激活端粒酶的过程中起限速作用，能以自身RNA为模板合成端粒序列以稳定端粒长度使细胞获得永生，也是人类端粒酶的活性中心，其表达是端粒酶活化的必须前题［2～4］。

Thus systematic identification of binding targets of transcription factors is key to unraveling gene regulation networks.

系统化的确定转录因子结合位点是揭示基因调控网络的关键。

Unstimulated resting cells served as control. The expression of CD154 mRNA was assessed by reverse-transcription PCR.

CD154 mRNA的稳定性用RT-PCR测定，并与不经刺激的T细胞作对照。

Immunofluorescence staining and reverse transcription polymerase chain reaction were used to test the expression of syndecan-1 protein and its mRNA in cultured unstimulated RPE cells.

2在正常RPE细胞，可检测到syndecan一1蛋白和mRNA的表达；第四军医大学硕士学位论文未受刺激的RPE细胞胞膜/胞浆、核仁呈现强的黄绿色荧光；随TNF一。

Attenuation The trp operon is also controlled by attenuation A leader sequence in the polycistronic mRNA upsteam of the coding region of the trpE structural gene encodes a 14 amino acid leader peptide including two tryptophan residues The RNA leader sequence can for several possible stem-loop secondary structures,one of which can act as a transcription terminator whilst a different stem-loop can act as an anti-terminator.

弱化作用 trp 操纵子也受到弱化作用的调控。在trpE结构基因编码区上游的多顺反子mRAN上有一段前导序列，可以编码一个含有14个氨基酸的前导肽，此肽中含有两个色氨酸残基。

Third partThe expression of HO-1 could inhibit the invasion of inflammatory cells, activation of transcription factor AP-1 and the production of cytokines, also could improve the oxdative stress state of the VILI and lessen the lung injury.

第三部分HO-1的表达增高能够抑制炎症细胞浸润、转录因子AP-1活化和细胞因子的产生，同时改善肺内氧化应激状态，可能是HO-1对VILI发挥保护作用的机制。

The effect of cyclin C on zygotic transcription activation during vertebrate embryonic development is poorly understood.

在脊椎动物胚胎发育过程中有关细胞周期蛋白C合子型转录激活机制尚知甚少。

The results of this study first pointed out that HMG transcription factor and GRP protein played a very important role during the development of somatic and zygotic embryo and CKⅡwas very important for somatic embryo development in plant.

本研究结果首次证实了HMG转录因子和GRP蛋白在植物体细胞胚和合子胚发生发育中及CKⅡ在植物体细胞胚发生发育中的重要作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。