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As an important component of transcriptional regulation, transcription factor binding sites identification has become a hot topic at present.

作为一种重要的转录调控元件，转录因子结合位点的识别已经成为当前的研究热点。

Transcription factor binding site discovery is an important step in understanding transcriptional regulation.

转录因子结合位点发现是理解转录调控中一个重要的步骤。

Expression of different stress-induced genes in plant is mainly regulated by specific transcription factors at the transcriptional level.

植物各种诱导性基因的表达主要受特定转录因子在转录水平的调控。

An in vitro transcription recombinant plasmid 5T1d was obtained after subcloning the target gene of clone 5T1d,which contained mutant HCV 5 non-coding region cDNA,into a transcriptive vector pCDNA Ⅰ.

采用含HCV5非编码区(5NCR)缺失突变的重组质粒5T1d，将其目的基因亚克隆到体外转录载体pCDNAⅠ多克隆位点上，获得转录重组体5T1d。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

Once activated,these two transcription factors translocate into the nucleus for inducing expression of INF-β,thus resulting in initiation of innate immune response and subsequent regulation of acquired immune response.

激活转录因子IRF-3和NF-κB，使其进入细胞核内，诱导干扰素的表达，从而启动固有免疫反应和调节随后的获得性免疫反应，增强机体抵抗病毒的能力。

Otch can translocate into nucleus and regulates downstream genes transcription. So it is suggested that AICD may play a role like NICD.

ICD会进入细胞核并对细胞进行生理调控，因此一般推测AICD可能具有类似NICD之角色。

These results suggested that the stimulation of CD44 plays an important role in tumor metastasis and CD44ICD may translocate to the nucleus as a transcription factor for a possible gene, MT1-MMP, to regulate CD44-dependent tumor cell migration.

而CD44ICD可能是藉由转移至细胞核内扮演标的基因MT1-MMP的转录因子，来调节由CD44所主导的癌细胞转移。

Based on high homology to an unannotated B. rapa BAC clone, primers were designed to amplify a 1566-bp region 5' flanking the transcription start site.

根据与一个未注解的白菜BAC克隆子的高度同源性，设计引物扩增了BnTT12-1转录起始位点5'的1566 bp序列。

To get more factors associated with erythropoiesis in K562 cells, modified differential display reverse transcription polymerase chain reaction method was applied to identify genes with differential expression in uninduced and hemin-induced K562 cells. Remarkable differential ESTs were firstly selected for cloning, sequencing and analyzed with bioinformatics methods.

为了进一步获得分化过程中相关基因表达的信息，我们应用改良的差异显示RT-PCR技术，分析K562细胞在Hemin诱导的红系分化前后的基因表达变化，获得红系分化相关基因的线索，进而确定在红系分化中起重要作用的基因，并研究它们的作用机制。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。