查询词典 transcription

The mechanisms of GSS revering the GR down-regulation occur at both the transcription and post-transcription levels.

人参皂苷逆转GR下调的机制发生在转录及转录后水平。

Borna disease virus ; Reverse transcription-polymerase chain reaction ; Receptor ; Serotonin ; Dopamine ; Transcription ; genetic

Borna病病毒；逆转录聚合酶链反应；受体；血清素；多巴胺；转录；遗传

Nuclear factor kappa B is a kind of eukaryon cell transcription factor which is transported to the cell nucleus by extracellular stimulus such as LPS, shock and so on through signal transmission pathway. It promotes and regulates the gene transcription of some inflammatory mediators and it closely relates to ALI.

核因子-кB是一种基因转录调节蛋白，内毒素、休克等细胞外刺激通过信号转导通路，引起NF-кB核易位，启动和调节多种炎症介质的基因转录，其活化与ALI的发病密切相关。

Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4／brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸，干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4／brn4的表达，免疫印迹检测反义干扰细胞POU3F4／brn4蛋白条带的强弱，RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化，观察POU3F4／brn4对COCH基因的转录调控作用。

The comparison of the three transcription factor families in homolog and expression patterns between grape and tomato suggested that these transcription fators in grape might play an important role in the regulation of fruit ripening.

比较葡萄与番茄中这三类转录因子的同源性和表达模式，我们发现，这些转录因子可能起着调控葡萄果实成熟的重要作用。

Indicates approximately how much text has been omitted from the transcription, in letters, minims, inches, or any appropriate unit, either because of editorial policy or because a deletion, damage, or other cause has rendered transcription impossible.

约略指出在转录当中省略的文字量，可用字母数、公厘、英吋、或其他适当的单位来表示。省略可能是出於编辑策略、来源文本中的删除、损毁、或其他因素而导致无法转录。

In exploring the mechanism underlying the decreased transcription of SPARC in NPC cells, we found that the transcription factor SRY (sex-determining region Y)-box 5 (SOX-5) is upregulated in NPC cells.

再经由定量聚合酶连锁反应(Quantitative RT-PCR)以及西方吸渍法的确认分析，发现 SPARC 基因在鼻咽癌细胞中的表现量显著减少。

In vertebrate organisms,thesignaling pathway is initiated by the binding of ligandsto the membranousreceptor patchedwhich in turn alleviates the suppression on smoothened,subsequently Smo triggers a series of intracellular events with resultant activation of thezinc-finger transcription effectors,glioma-associated oncogenes(Glil,Gli2,Gli3)transcription factor,which induces the expression of numerous target genes,such as Ptch-1,Hip,Gli1 and Wnt,that regulate proliferation,differentiation.

Hedgehog信号通路是高度保守的调控胚胎组织分化发育的通路，在脊柱动物体内，Hh蛋白与跨膜受体Ptch结合后，解除了Ptch对Smo的抑制，随后Smo从共受体上解离下来进入胞浆，将信号下传并激活锌指转录因子Gli，后者启动Ptch-1、Hip、Gli1、Wnt等目的基因的表达，进而调控细胞的增殖、分化。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

And ZNF397nf function as transcription repressors. The different transcriptional regulation activity of the nf isoforms and their interaction with the fu isoforms would contribute to the complexity of the transcription regulation. To further examine the biological function of interaction between different isoforms we also analyze the potential role of ZNF191 and ZNF434 on signaling pathway. We found that ZNF191fu can significantly enhance the HSE-luciferase activity, while ZNF434fu can enhance the AP1-luciferase activity, then we analyze whether the nf isoforms have impact on the fu isoforms.

而这些成员的nf剪接本与它们对应的fu剪接本的转录调控功能有所不同，其中ZNF191nf与ZNF434nf剪接本的转录调控活性相对它们的fu剪接本显著降低，呈现较弱的转录抑制作用，但ZNF447nf剪接本却呈现出显著的转录激活作用，同时ZNF397nf剪接本则表现出显著的转录抑制作用，ZNF396nf剪接本的转录调控作用与其fu剪接本相当。nf剪接本这种与fu剪接本不尽相同的转录调控活性以及能与fu剪接本互作的能力，大大增加了转录调控作用的复杂性，这使得通过少量的转录因子作用来实现众多基因时空表达的不同成为可能。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。