查询词典 transcription

Methods: The cells were treated with the different concentrations of Cobaltous chloride. The lactic acid dehydrogenase from the supernatant of the cells was detected by a biochemical analysis. The cells were co-transfected with a pGL2-eNOS-p vector while stimulated with Cobaltous chloride at the different concentrations and disposing time, and the transcription activity of human eNOS promoter was determined through using a double luciferase reporter gene system.

用含不同浓度氯化钴的培养基培养细胞，检测细胞培养上清中的乳酸脱氢酶含量；将已构建的pGL2-eNOS-p质粒转染HUVEC-12细胞，利用双荧光素酶报告基因技术检测在不同浓度氯化钴和不同作用时间下的eNOS启动子转录活性。

Transcription of received volumes must be current, with at least three true copies of all new works, and four true copies of Compendia.

当前那些已被认可的卷宗必须抄写，至少把所有的新作品精确抄写三份，大纲则精确抄写四份。

Transplantation of human neural stem cells transduced with Olig2 transcription factor improves locomotor recovery and enhances myelination in the white matter of rat spinal cord following contusive injury.

BBB评分法专门用于评价大鼠胸髓损伤后后肢功能恢复情况；每一个分数都有一个独立的标准；评分还包括许多动物行为评定细节和特征说明；评分的渐进性排列，体现了脊髓损伤恢复的全过程；行为学研究表明该标准较为客观，已被广泛应用。

The expression profiles analysis of 25 genes belonging to 8 types in different development periods of Y35 somatic embry showed, genes involved in transcription, signal transduction, cell fate, substance transport, cell and development regulation were active at early and middle stages of somatic embryogenesis, while at the stage of cotyledonary embryo maturation, the high level-expressed genes were mainly those related to storage protein, maturation and defense.

通过半定量和Real-Time PCR技术，对WRKY和MAPKK等8类25个差异表达基因在Y35体细胞胚不同发育时期的表达分析显示，转录、信号转导、细胞生长分裂、细胞命运、物质转运和多数发育调节相关基因在体细胞胚胎发生的早期或中期比较活跃，而进入子叶胚成熟后期则主要是储藏蛋白、成熟和防御相关基因大量表达。

The reverse transcription polymerase chain reaction RT-PCR and cDNA microarray were used to evaluate VEGF-C mRNA expression of T24 cells at around the best effect time and as the Crocin concentration reached the best.

用RT-PCR和基因芯片分别检测最佳作用时间和最佳药物浓度作用前后T24细胞VEGF-C的表达。

Methods The authors studied the expression of CyP gene from white blood cells in children with nephrotic syndrome by reverse transcription polymerase chain reaction.

采用逆转录聚合酶链反应测定28例肾病综合征患儿血白细胞中CyP mRNA的表达，并以正常儿童作对照。

In othor way, we use RT-PCR to observe the secretion of mRNA to compare with the balance of TH1/TH2 in spleen, so that we can see if we treat with cystamine can apparently raise the ratio of TH1 and also raise mRNA of CD25/ Foxp3, but reduce mRNA of CD40L. As a result of kidney which is the important secondary immunity organ discusses cystamin whether is the important control action or not to regulate in kidney then to be able to improve illness.

另外，透过了反转录聚合链锁反应(reverse transcription-polymerase chain reaction， RT-PCR)观察spleen中CD8/CD4 maker的mRNA表现量比较TH1/TH2 balance的倾向，发现经过胱胺处理后的确TH1相关的CD8 maker的mRNA比例量有大幅度上升，间接证明SLE倾向於TH2有被反转，而在Treg的特异性 maker方面CD25/ Foxp3之mRNA之比例也有大量上升，T cell的活性化maker CD40L也减少了mRNA的表现量，同时证实了SLE免疫倾向的反转，及发炎情况的减少及Treg cell的大量诱发。

The expression levels of Nup88, Nup214 and NPM were decreased in a dose-dependent manner both at protein level and transcription level when cultured with deguelin.

其抗白血病效应可能与其下调核孔蛋白Nup88、Nup214以及核磷蛋白NPM的表达有关。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

The inhibitor for DNA methyltransferase, 5-azacytidine (5-azaC), can demethylate the methylated DNA and restore transcription of genes. High dose homocystine can mediat DNA methylation and suppress gene expression.

应用甲基转移酶抑制剂5-氮杂胞苷(5-azacytidine， 5-azaC)可以使甲基化的启动子去甲基化，启动基因重新转录；高浓度同型半胱氨酸(homocystine， Hcy)可以介导基因甲基化而抑制其表达。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。