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Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

The lack of liver and kidney type PBMC in patients with alopecia areata Thl/Th2 type cytokine gene expression imbalance, presented to Th1-type cytokines and transcription factor T-bet gene over-expression of Th1-type response characteristics, may be involved in the pathogenesis of alopecia areata, alopecia areata may be insufficient evidence of liver and kidney-shaped one of the micro-nature.

肝肾不足型斑秃患者PBMC中Thl/Th2型细胞因子基因表达失衡，呈现以Th1型细胞因子和转录因子T-bet基因过度表达为特征的Th1型反应，可能参与了斑秃的发病机制，也可能是斑秃肝肾不足证型的微观本质之一。

This is an interesting transcription of what actually occurred from Koala Bear's point of view.

这是来自树袋熊对于真实发生过的事件的一个有趣转译。

Using reverse transcription PCR, kininogen mRNA was also detected in lamprey gut, kidney, and leukocyte, but absent in lamprey buccal gland.

运用反转录PCR技术，检测出激肽原还存在于七鳃鳗的肠、肾和白细胞中，但不存在于口腔腺中。

A Plasmid DNA vaccine pcDNA-gB expressing gB protein of infectious laryngotracheitis virus Yantai strain was constructed. The expression of viral protein from constructed DNA vaccine was confirmed by an in vitro transcription /translation system and transtection in 293-T cells.

本研究构建了编码ILTV主要抗原gB基因的重组DNA疫苗pcDNA-gB，质粒转染293-T细胞的间接免疫表明其表达的蛋白具有免疫反应性。

Since respiratory tract infections, including IB, are critical in the poultry industry, a multiplex reverse transcription-polymerase chain reaction assay that detects avian viral respiratory pathogens in the same reaction was developed for the economic and clinical needs. Four sets of specific oligonucleotide primers for infectious laryngotracheitis virus, IBV, Newcastle disease virus, and avian influenza virus were used in this study.

由於呼吸道疾病对於家禽产业有很大的影响，因此基於经济与应用在临床上区别诊断的考量，本实验室发展多引子反转录聚合酶链锁反应，一共使用四组针对传染性喉头气管炎病毒、传染性支气管炎病毒、新城病病毒、以及家禽流行性感冒病毒的特异性引子来进行测试。

It can promote flowering in some late-flowering ecotypes and many late-flowering mutants to decrease the level of FLC transcription and expression in the plant.

FLC是拟南芥中抑制成花的基因，通过负调控FLC的转录及蛋白表达水平，促进拟南芥的某些晚花生态型和晚花突变体开花。

The promoter is typically haloarchaeal, but the start site of transcription is only seven bases from the 5'end of the initiator AUG codon, making the halC8 transcript another example of a"leadless transcript"in the haloarchaea.

它的-29～-24位置上是一"TATA"box序列，为典型的嗜盐古菌启动子；它的转录起始位点距离"ATG"只有7个核苷酸，也是典型的嗜盐古菌的"leadless transcript"。

Various studies indicate that leaf de-velopment is controlled through the interaction of transcription factors, small RNAs and auxin.

文章综述了近年来叶发育和形态建成的分子机制研究进展，以期了解叶发育的调控网络。

In this study, these factors and enzymes are detected on transcription level in mice adipose tissue, liver and adipocyte, which were treated by different concentration of DHA. We also analysised the correlation between adipocyte proliferation and differentiation and SREBP-1c, FAS and HSL gene expression, and tried to explore the effects of DHA on lipogenesis and lipolysis genes.

本试验以小鼠脂肪组织和肝脏及脂肪细胞为研究对象，研究DHA对小鼠生脂基因PPARγ、SREBP-1c、FAS、HSL和TGH转录表达的短期和长期效应以及脂肪细胞增殖分化和脂代谢的影响，探讨DHA对脂肪生成及分解的作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。