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With retro-transcription PCR method, we cloned cDNA of iron-superoxide dismutase from the seeds of camphor tree.

本文报道了关于樟树种子的铁超氧化物歧化酶基因的克隆，表达，金属离子配基置换以及高级结构模拟方面的工作。

Transcription factor E2F 1 is related to the cell proliferation,apoptosis,aging and canceration of renal intrinsic cells.

转录因子1（E2F-1）与肾固有细胞增殖、凋亡、衰老及癌变有关。

Objective To evaluate the reliability of reverse transcription -PCR in detecting the expression of the carbonic anhydrase Ⅸ gene in renal cell carcinoma.

目的评价RT-PCR法检测碳酸酐酶Ⅸ基因在肾癌组织的表达的可靠性。

The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达，酶联免疫法检测培养上清液中碳氧血红蛋白含量，四唑氮比色法检测细胞增殖。

It plays an important role in the regulation of many skeletal muscle- and cardiac muscle-specific gene expression and transcription.

通过与基因启动子中特异序列结合，从而发挥对肌细胞特异性基因表达、转录的调控作用。

Used to enhance transcription of the lac and ara operon genes, which produce proteins involved in catabolism.

用于增强lac和ara操纵子基因的转录，产生的蛋白在分解代谢中起作用。

CRP/CAP Catabolite activator protein,CAP is an activator required for high level transcription of the lac operon.The active molecule is a CRP dimer that binds 3'5' cyclic AMP to form a CRP-cAMP complex.

CRP/CAP 分解代谢物激活蛋白CAP是Lac操纵子高水平转录的一个必须的激活因子，有活性的CRP二聚体与3ˊ5ˊ-环式AMP结合形成CRP-cAMP复合物。

Histone deacetylase 6(HDAC6) is microtubule and HSP90 associated protein, which control genetic transcription and change cellule motor ability by deacetylased microtubule and HSP90,and then participate tumorigenesis, growth, metabasis, and the production of tumor vessel.

研究方法： 1。应用激光捕获显微切割术联合逆转录-聚合酶链反应技术，检测36例行根治性切除的原发性肝细胞癌中心处非坏死癌组织及24例正常肝组织的HDAC6 mRNA在组织中的表达，并对HDAC6与肝癌发生发展过程中相关的临床病理因素间相关性进行研究。

The recombiant protein with GST tag was soluble. SjMRLC showed similar transcription level in all stages including sporocyst, miracidium, egg, cercaria, schistosomulum, female worm, male worm, and paired adults.

结果 构建pGEX 4T-3/SjMRLC重组质粒，测序表明插入的外源基因片段正确，诱导表达获得带有GST标签的可溶性蛋白（Mr 49 000）；虫期转录特异性分析显示该基因在日本血吸虫各个虫期均有转录，且水平一致。

Methods The expression of mdr1 and MRP were measured in 55 patients with AL by reverse transcription polymerase chain reaction.

采用逆转录-聚合酶链反应的方法检测55例AL患者的mdr1及MRP的表达。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。