查询词典 transcription

On the other hand, flow cytometry was employed to detect the action of paclitaxel to cell cycle and apoptosis. Reverse transcription polymerase chain reaction is employed to detect the influence of paclitaxel on Survivin mRNA expression. The purpose of the study is to provide theoretical basis to carcinogenesis, progress, metastasis, angiogenesis and clinical chemotherapy of paclitaxel on TSCC.

采用流式细胞仪检测紫杉醇对舌癌细胞细胞周期的影响及应用逆转录聚合酶链反应(reverse transcription polymerase chain reac-tion，RT-PCR)检测紫杉醇对舌癌中Survivin mRNA的影响，为研究舌鳞癌的发生、发展机制，舌癌的转移及新生血管形成，及临床应用紫杉醇抗舌癌提供理论依据。

POU1F1 is the first certified pituitary transcription factor, it plays a role in control ofgrowth process and metabolism, it has far-ranging biological functions in improvinggrowth, reducing fat aggradation and so on.

POU1F1(pituitary transcription factor 1)是被鉴定的第一个垂体转录因子，它对哺乳动物的生长发育、新陈代谢起重要的调节作用，具有提高生长速度，降低脂肪沉积等广泛的生物学功能。

Based on the understanding of physiological pathway for broodiness, three genes related to broodiness including PRL, PRL receptor, and pituitary-specific transcription factor (POU1F1)-encoding genes were selected as candidates to screen the genomic variation by means of PCR-SSCP protocol and analyze the association with chicken broodiness and other target traits.

禽类血浆PRL水平上升与就巢行为的起始和维持具有密切的相关性，本研究采用候选基因的策略，通过分析就巢行为发生的生理机制，选择与就巢性状密切关联的PRL、PRL受体和PRL转录调控因子——垂体特异转录因子(pituitary-specific transcription factor，POUlF1)基因，采用PCR-SSCP分析检测基因变异，并分析基因变异与就巢性和其它目标性状的相关性。

By interacting with these proteins, PIAS proteins could affect their activation and function, and take part in gene transcriptional regulation and cellular signal pathway including Signal transducer and activator of transcription, Wnt, TGFβ, NF-κB pathways and so on, therefore present important biological functions.

通过相互作用，PIAS蛋白通过影响了蛋白的活性和功能，参与基因的转录调控和包括STAT(Signal transducer and activator of transcription)， Wnt， TGFβ， NF-κB等通路在内的细胞信号通路，具有重要的生理功能。

KCR and HCD are erucic acid synthesis genes. In order to reveal the relationship between the synthesis of erusic acid and the quantitative expression of its functional genes, and to reveal the theoretic base of the breeding for high erucic acid content, the expression of KCR and HCD had been analyzed kinetically and quantitatively by using regular reverse transcription polymerase chain reaction and the internal standards of QuantumRNA18S during the growing period of high erucic acid content lines of Brassica Juncea.

研究应用常规RT-PCR(Reverse transcription polymerase chain reaction)的技术和QuantumRNA18S内标对芥菜型高芥酸油菜生育期内芥酸合成基因KCR、HCD基因的表达进行量化分析，了解芥酸的合成与功能基因量化表达的关系，为芥菜型油菜的高芥酸品种的选育提供理论依据。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

We aim to screen and identify the key potential trans-factors during hemoglobin switch. We firstly analyzed differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal umbilical cord blood, adult bone marrow, and bone marrow of a heterocellular hereditary persistence of fetal hemoglobin patient. We identified ZF (HCF-binding transcription factor, Zhangfei) and SH3GLB1(SH3-domain GRB2-like endophilin B1) that had differential expression in the above three cultures. Furthermore, we confirmed the different expression of the above two genes by quantitative real-time PCR.

为鉴别影响珠蛋白基因表达和开关的新的重要调节因子，我们首先分析了人脐带血、正常成人骨髓和异细胞型胎儿血红蛋白持续存在综合症患者骨髓细胞红系诱导培养物中基因表达的差异，发现ZF(HCF-binding transcription factor，Zhangfei)和SH3GLB1（SH3-domain GRB2-like endophilin B1）在这三种不同来源的红细胞内具有显著的表达差异，并通过实时定量PCR方法验证了差异的真实性。

Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23 A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells.

结果：筛选出21个差异蛋白质点，成功鉴定出5种差异蛋白质，其中在LoVo细胞株中表达上调的蛋白有埃兹蛋白、转录延长因子A (transcription elongation factor A)和甘氨酸A/C，在SW480细胞株中表达上调的蛋白有NM23蛋白[non-metastatic cell 1 protein (NM23A) expressed in isoform a]和（heat shockprotein 8，热休克蛋白8）。

Result: A 77-year-old female presented at our hospital for blurred vision in her right eye. The intraocular pressure was 46 mmHg and slit lamp examination showed a protruding mass with papillomatous appearance near pupil margin. On chest X-ray examination, only prominent left hilum was noted. Iris tumor excisional biopsy was performed. Pathology investigation of the surgical specimen revealed carcinoma and immunohistochemical study demonstrated thyroid transcription factor-1 (TTF-1) positive. Chest-computed tomography showed left upper lung tumor and primary pulmonary adenocarcinoma was confirmed after CT-guided biopsy. She was placed under treatment with Genfitinib. Recurrent iris tumor mass was noted during follow-up, and chest CT revealed shrinkage of adenocarcinoma.

结果：一位七十七岁女性因右眼视力模糊至本院就诊，当时眼压为46毫米汞柱，细隙灯检查发现虹膜靠近瞳孔边缘有一乳突状肿块，胸部X光摄影仅见左肺门稍明显并未发现明显病灶，患者接受虹膜肿瘤切除手术，病理报告显示为恶性肿瘤，且免疫组织染色为thyroid transcription factor-l阳性，进一步的胸部电脑断层显示左上肺肿瘤，经电脑断层指引之切片检查证实为原发性肺腺癌，她接受Genfitinib药物治疗，后续追踪发现虹膜肿瘤复发，胸部电脑断层扫描显示肿瘤体积缩小。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。