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Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

A significant decrease in BOULE transcript levels was detected in patients with spermatogenic failure, and BOULE transcript levels progressively decreased with increasing severity of testicular failure.

研究结果显示在造精障碍的患者中 BOULE 转录量显著的降低，而且随著造精障碍的严重程度增加，BOULE 转录量就更显著的下降。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

The promoter is typically haloarchaeal, but the start site of transcription is only seven bases from the 5'end of the initiator AUG codon, making the halC8 transcript another example of a"leadless transcript"in the haloarchaea.

它的-29～-24位置上是一"TATA"box序列，为典型的嗜盐古菌启动子；它的转录起始位点距离"ATG"只有7个核苷酸，也是典型的嗜盐古菌的"leadless transcript"。

SW480 and SW620 cells were also used to study the gene regulation with high carbohydrate intake, where it was shown that during glycolysis eight genes, HK1 (nuclear gene encoding mitochondrial protein, transcript variant 1), GPI, GAPD (glyceraldehyde-3-phosphate dehydrogenase), PGK1 (phosphoglycerate kinase 1), PGK2 (phosphoglycerate kinase 2), ENO2 (enolase 2), PKM2 (pyruvate kinase, muscle, transcript variant 2) and GLUT1 (facilitated glucose transporter, member 1) are over-expressed. In our study, we also found that during hypoxia of cancer tissues the resulting up-regulation of HIF-2α(hypoxia-inducible factor 2, alpha subunit) would in turn up-regulate the GLU1 gene, further activating glycolysis.

另一方面，在高碳水化合物的调控研究上，吾人也利用SW480和SW620细胞株分析证实醣解水解活化过程中，其六碳醣激酶-1(HK1)、葡萄糖磷酸异构酶、3-磷酸甘油醛脱氢酶、磷酸甘油酸激酶-1(PGK1)、磷酸甘油酸激酶-2(PGK2)、烯醇酶-2(ENO2)、丙酮酸激酶-2(PKM2)以及葡萄糖输送蛋白-1(GLUT1)等共有8个基因是连续上升的过度表现；此外，吾人也发现当癌细胞在缺氧的组织中，其缺氧转录子-2α基因(HIF-2α)表现上升后，会活化GLU1基因上升，进一步活化醣解代谢的进行。

In accordance with the requirements of the hearsay evidence rule, the survey notes recording the integrator's investigation results do not have consequent provability , only the investigation officer as witness in the trial accepts questioning and anti-questioning and assure that the investigation transcript was made on the basic of right observation and awareness, can the transcript be used as evidence.

根据传闻证据规则的要求，记录刑侦人员勘验调查结果的笔录，不具有当然的证据能力，只有该勘验，侦查人员在庭审时作为证人接受询问和反询问，并陈述确实系他根据正确的观察和认识而制作成的，才能作为证据使用。

In order to understand these differences on gene transcript level, even to get the genes and their expression related to cell culture, we researched the differences of total mRNA of these tissues by RAPD-PCR, the result showed that the difference between embryo and head of nymphae was much closer than embyo with others, which was accorded to the time order of insect upgrown, and the tissue which is more different from embryo on transcript level ismore diiBcult to be cultured in vitro.

四种组织的培养难易程度和分离出的细胞形态的不同，说明存在着组织表达的差异，为了从转录水平上来认识它们的差异，甚而获得与细胞培养难易相关的基因及其表达产物，本实验还采用了RAPD分析的方法进行了这四种组织：中后期虫卵胚胎、若虫头部组织、成虫的精巢和卵巢的mRNA表达差异研究，结果表明：从发育方向上看，四种组织的表达差异有分化越大差异系数越大的趋势，而在相同条件下则越难培养。

Although the larger transcript is expressed at low levels in other tissues, the smaller transcript is expressed exclusively in spermatids, suggesting GCNF may play roles during the development of sperm cells.

虽然7.4Kb的大转录子在其它组织如脑、肝等中有低水平的表达，但2.3Kb小转录子则专一性地在生殖细胞中表达，因此GCNF可能在精细胞的发育过程中起到重要作用。

Objective: To investigate the effect of glucocorticoid on expression of hypothalamic Agouti-related peptide, and cocaine and amphetamine regulated transcript in normal and obese rats.

目的：探讨糖皮质激素对普通大鼠和肥胖大鼠下丘脑Agouti相关蛋白和受可卡因和安非他明调节的转录肽基因表达的影响。

26 Am Ian studies his transcript and diploma as he departs the Bear Creek High School commencement at Red Rocks Amphitheatre.

上午11时26伊恩研究，他的成绩单和文凭，他离开了熊溪中学的红色岩石露天剧场展开。

"Vina Express" refers to the freight forwarding name?

"Vina Express"指的是货运代理的名字吗？

Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage.

珍珠层粉在新西兰兔股骨髁内的成骨试验。