查询词典 transcribed

Methods According to differences in internal transcribed space sequences of Penicillium genus and specific IAO sequence, we designed species-specific primers AS1/RS4 and IAO1/IAO2 of Penicillium griseofulvum and established the corresponding PCR systems.

根据 GeneBank 中青霉属不同种和相近属种的 ITS 序列差异和灰黄青霉特有的 IAO 序列，设计了污染区优势种灰黄青霉的特异性引物 AS1/RS4 和 IAO1/IAO2，建立相应的特异探针检测体系。

Results (1)Multiple copies of pMCLacI/Neo plasmid DNA were intactly integrated in the genomic DNAs of the cell clone.(2) One of lac Ⅰ target genes in the integrated plasmid could be transcribed in the NIH3T3 cells while the other could not.

因此，在这些突变婴儿检测系统中，通过一些不表达的中性靶基因所婴儿检测到的突变规律肿瘤是否代表体内一些重要的功能基因的真实情况，目前尚不清楚［1-3］。

Results (1)Multiple copies of pMCLacI/Neo plasmid DNA were intactly integrated in the genomic DNAs of the cell clone.(2) One of lac Ⅰ target genes in the integrated plasmid could be transcribed in the NIH3T3 cells while the other could not.

因此，在这些突变检测系统中，通过一些不表达的中性靶基因所检测到的突变规律是否代表体内一些重要的功能基因的真实情况，目前尚不清楚［1-3］。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

Results They could find the influence of pseudogene through performing genomic DNA and nonreversed transcribed RNA controls.

结果 通过设立分别以基因组DNA和未经逆转录的RNA为模板的对照可观察到CK19假基因的干扰现象。

The sequences of internal transcribed spacer regions of nuclear ribosomal DNA of 9 samples of Nymphaea and related water lilies as outgroups were sequenced and assembled with the sequences in GenBank to analyze the specific and infraspecific relationships.

本文测定了睡莲属9个样品的核核糖体DNA ITS区序列，结合GenBank中获得睡莲类ITS序列，分析了这些样品间的亲缘关系。

Ladeuze in Revue biblique, 1902, pp 573 sq. Grammatically, this phrase corresponds perfectly with the Apostle's style (cf. Galatians 1:22; 1 Corinthians 1:2; Philippians 1:1) and palaeographically, if transcribed in ancient capitals, it readily accounts for the corruption that has certainly been produced in the text.

Ladeuze杂志biblique在1902年，第573平方米）语法，这句话完全对应的使徒的风格（参看加拉太1时22分；哥林多前书1：2 ；腓1：1 ）和palaeographically ，如果转录的古都，它随时帐户腐败无疑是产生於文字。

Methosd The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product .

先从并殖吸虫病患者痰中分离出虫卵，然后PCR扩增出虫卵中完整的核糖体DNA第二间隔区基因(ITS2 )，并直接用于测序从而获得该基因的核苷酸序列。

The phylogeny of the genus Machilus was analysed by means of Bayesian Inference of nrDNA ITS sequences . Our results indicated that Machilus is a monophyletic clade within the Persea group, and its persistent and spreading to reflexed tepals on fruit are an important character for its generic delimitation against the related genera Phoebe, Alseodaphne and Persea .

应用nrDNA Internal Transcribed Spacer 序列，使用Bayesian 分析法对樟科润楠属的系统学问题进行了初步探讨，结果表明润楠属在鳄梨属群中本身作为一独立分支，是一个自然的单系类群；由于其花被裂片果期宿存且强烈反转与同产自亚洲的楠属和油丹属存在清晰界线。

A device that checks the correctness of transcribed data, usually by comparing with a second transcription of the same data or by comparing a retranscription with the original data.

核对转录的数据是否正确的一种设备，通常的做法是：将转录好的数据与同一数据的第二次转录进行比较，或令转录好的数据的再次转录与原数据进行比较。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。