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In order to clarify the taxonomic status of Dirofilaria sp. of Ailurus fulgens,the second internal transcribed spacer (ITS-2) of rDNA of Dirofilaria immitis from dogs in Ya'an in Sichuan Pro-vince and of Dirofilaria sp.from Ailurus fulgens in Chengdu and Chongqing were sequenced and compared.

为探讨采自小熊猫的恶丝虫虫种的分类地位，测定了四川雅安地区犬源犬恶丝虫和成都及重庆两地小熊猫源恶丝虫的核糖体第二内转录间隔区（ITS-2）基因序列并进行了比对分析。

Methods Viruses were passed in embryonated hen eggs, and virion RNA was extracted from allantoic fluid and reverse transcribed to synthesize cDNA. cDNA was amplified by PCR and the PCR product was purified with a purification kit.

方法病毒在鸡胚中传代，从收获的尿囊液中提取病毒粒RNA，通过逆转录合成cDNA.cDNA通过PCR进行扩增，接着PCR产物用纯化试剂盒进行纯化。

This paper reviewed the application of nuclear ribosomal DNA internal transcribed spacer region sequences in phylogenetic study of angiosperms at intra\|inter genera or family levels in recent years at home and abroad. Combined with the research of Chinese \%Alpinia \%Roxb.

本文就近年来国内外有关被子植物核糖体DNA中的内转录间隔区序列在植物属内、近缘属间乃至科内系统发育研究中的应用，结合作者在中国姜科山姜属 AlpiniaRoxb 。

Armillaria luteo-virens is a ectomycorrhizal fungus symbiotic with some species of Kobresia in Tibet Plateau, China. A strain was recently isolated from fruitbody tissue of the fungus. To determine whether the strain is the pure culture of the fungus, the internal transcribed spacer region and the first intergenic spacer region (IGS-1) of the nuclear ribosomal DNA of fruiting body and the isolated strain were sequenced and compared. Further, the 5.8S/ITS and IGS-1 sequences of the species were BLAST matched with GenBank database on homology and a phylogenetic tree was constructed based on 5.8S/ITS sequence and two phylogenetic trees were constructed separately based on two IGS-1 sequences.

首次从采自青藏高原、与高原牧草嵩草属Kobresia草本植物形成外生菌根的黄绿蜜环菌Armillaria luteo-virens子实体中分离获得一组织分离菌株，运用rDNA-ITS和rDNA-IGS-1测序技术对该组织分离菌株是否为黄绿蜜环菌的纯培养菌种进行分子鉴定，并基于黄绿蜜环菌的5.8S/ITS和IGS-1序列进行核酸序列数据库GenBank同源性检索比对、构建系统发育树。

The internal transcribed spacer region in rRNA gene of Auricularia polytricha was cloned and sequenced, and the sequences were compared with those of some common species of Auricularia.

对毛木耳7个菌株rRNA基因内转录间区进行了克隆测序，并将其与木耳属其它几种的相应序列进行了比较。

Transcribed by Deacon Jim Awalt.

转录由执事吉姆埃瓦尔特。

Anastomosis group identification and 5.8S rDNA-internal transcribed spacer sequence analysis showed that the isolates belonged to multinucleate Rhizoctonia AG1-IA, AG1-IB, AG4-HG-I, AG-5 and WAG-Z and binucleate Rhizoctonia AG-A and AG-Ba.

融合群测定及5.8S rDNA-ITS区序列分析结果表明，这些菌株分别属于多核丝核菌的AG1-IA、AG1-IB、AG4-HG-I、AG-5、WAG-Z融合群及双核丝核菌的AG-A、AG-Ba融合群。

Total RNA was substracted from the 5th insar nymphs of both macropterous and brachypterous strains The actin gene mRNAs were divided into three groups when reverse transcribed into cDNAs.

本实验还提取了褐飞虱若虫的总RNA，在反转录过程中，利用锚定的3'RACE方法，将所有的mRNA分成了三组，并反转录成cDNA。

Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proc Natl Acad Sci USA ［J］.1987, 84 (5):9054.［3］ Ainouche M L, Bayer R. On the origins of the tetraploid Bromus species: insights from internal transcribed spacer sequences of nuclear ribosomal DNA［J］.

而山西平遥产山药D= 0.004 76与山西太谷产山药D= 0.004 27基本没有差异，它们和河北安国产山药D= 0.004 36共同与怀山药有较大的同源性，通过ITS序列分析，这几种不同产地的山药之间碱基仅相差几个，提示它们具有同源性；野生山药与种植山药碱基数目相差较大，说明种植山药在驯化过程中产生了变移，使物种更进化。

In this study, the spore germination of Lasiosphaera fenzlii and Calvatia gigantea were studied,the optimum fermented conditions were investigated.The anti-inflammation and antibacterial were carried out using the fermentative products. The results were as follows: Molecular identification techniques using DNA showed that samples collected are Lasiosphaera fenzlii and Calvatia gigantean.Using analysis of Internal transcribed sequence and construct phylogenetic trees,results showede that Lasiosphaera fenzlii and Calvatia gigantean have close relationship.

本研究以大秃马勃Calvatia gigantea和脱皮马勃Lasiosphaera fenzlii为研究材料，进行了马勃孢子的萌发条件研究，并以大秃马勃Calvatia gigantea菌种为研究材料，进行了优化培养和发酵液药理实验的初步研究，结果如下：采用DNA分子鉴定技术对采集的样品进行了分子鉴定，结果为大秃马勃Calvatia gigantea和脱皮马勃Lasiosphaera fenzlii。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。