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These ingredients includes epidermal growth factor, hepatocyte growth factor, dimethyl sulfoxide, phenobarbital sodium and all trans-retinoic acid. We isolate and culture primary 10-weeks-old human fetal hepatocytes, observe hepatocytes form and its function markers. Inoculate hepatocytes with HBV Dane particles to observe if HBV susceptibility of fetal hepatocytes can be induced in vitro. To detect HBV covalently closed circular DNA within hepatocytes, we improve the existent PCR method.

基于上述假设,我们设计了10种培养条件,在自行设计的无血清培养基中添加不同的细胞分化诱导物,包括表皮生长因子、肝细胞生长因子、二甲基亚砜、苯巴比妥钠、全反式维甲酸等,分离孕10周龄人胎肝细胞进行原代培养,观测肝细胞形态及功能指标变化,并定时给予HBV感染,观察能否在体外诱导胎肝细胞出现HBV易感性;为检测肝细胞内HBV共价闭合环状DNA(covalently closed circular DNA,cccDNA),我们改进了现有的PCR检测法。

Secondly,a new capacitive trans-impedance amplifier is proposed to convert the photocurrent generated by weak fluorescence to voltage.

其次,本文提出一种新型的电容跨阻放大器结构读出电路来实现微弱荧光产生的光电流到电压的转换。

We pick up the story going to the train station in Khabarovsk to catch the Trans-Siberian Railroad.

我们拿起故事前往火车站,在哈巴罗夫斯克,以赶及在横贯西伯利亚的铁路。

Domestic and international large-scale enterprise group, general headquarters, regional general headquarters, research and development centre, marketing centre, gather to above-mentioned in the center that metropolis enclose constantly of trans-corporation, Accelerate coastal cities paces of internationalization , become various kinds of resource, key element in two market pivot that butt joint blend.

国内外大型企业集团,跨国公司的总部、地区总部、研发中心与营销中心、将不断向上述大都市圈的中心汇集,加快沿海城市国际化的步伐,成为各种资源、要素在两个市场对接交融的枢纽。

This article here analysis on the trans- plantation of some legal issues, and put forward my views.

本文在这里对法律移植的某些问题做分析,基此提出自己的观点。

But to many people, the 1982 Pontiac Trans Am was the original, and that's all that matters.

但对很多人来说,1982年庞蒂亚克跨时是原来的,这是所有的事项。

Urban and zone trarspertalin planning ; feasible study and the later evaluation for the projects at trans potation constracture ; urban traffic contral and management

城市与区域交通规划、交通建设项目工程可行性研究及评估,城市交通控制与管理。

Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon ( Pf ., Tg ., Tv .) and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点,与华支睾吸虫LDH同源性最高为75%,与阴道毛滴虫LDH同源性最低为17%,与人LDH(HsLDH-A,-B,-C)的同源性为58%~60%; SjLDH与果蝇的进化距离较秀丽隐杆线虫为近,3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近;该蛋白具有3个跨膜区域,3个高亲水性区域,主要的线性表位98aa~106aa位于膜外,与原虫LDH相同区域差异显著,而与其他LDH有1~3个氨基酸的差异,关键催化位点之一及底物丙酮酸结合区域位于该区域,蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构,3个关键催化位点位于该区域或在其附近。

The oil company British Petroleum has shut down a pipeline connecting the Prudhoe Bay oil fields in Alaska to the Trans-Alaska pipeline .

英国的石油公司关闭了一条连接阿拉斯加和普拉底霍湾的石油管道。

Pattison, Neural network for quadratic optimization with bound constraints, IEEE Trans.

分析:前为引用作品的内容,里面则为引用的作品号。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。