查询词典 toxin

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

The book comprehensively reviews the molecular and cellular biology of the most important microbial toxins with a focus on recent advances in the field, covering topics such as toxin ADP-ribosylation of proteins, toxin inhibition of protein synthesis, modulation of actin cytoskeleton, cytolethal distending toxins, cell-damaging/pore-forming toxins, clostridial neurotoxins, toxins of the immune system, streptococcal and staphylococcal toxins, anthrax toxins, Shiga toxin, Yersinia toxin, mycotoxins, and the regulation of toxin expression, as well as the potential of microbial toxins in bioterrorism.

该书全面评论了最重要的微生物毒素的分子与细胞生物学，重点是这一领域中的最新进展，主题包括蛋白质毒素ADP核糖基化，毒素抑制蛋白质合成，肌动蛋白细胞骨架的调控，细胞致死增加毒素，细胞损伤/孔状毒素，梭菌属的神经毒素，免疫系统的毒素，链球菌和葡萄球菌毒素，炭疽毒素，Shiga毒素，Yersinia毒素，毒枝菌素，和毒素表达调节，以及生物恐怖中微生物毒素的潜在可能性。

PAL activity, chitinase activity, total Polyphenol content and total Flavonoids content in soybean leaves induced by the same crude toxin and race were similar, it stated that the crude toxin is an important factor which can induce resistant substances, but the induced speed and the induced intensity by the crude toxin and conidium were different. Firstly, the induced speed of crude toxin which induced PAL activity and total Flavonoids content of resistant soybean varieties was faster than speed of C.

同一生理小种的粗毒素与灰斑病菌对大豆叶片内PAL活性、总多酚类含量、总黄酮含量以及几丁质酶活性等几种生化指标均表现出相似的诱导作用，由此可推断粗毒素是诱导大豆叶片内的PAL活性、总多酚含量、总黄酮含量以及几丁质酶活性产生变化的主要生物因子，只是二者在对抗性不同的大豆品种的诱导速度及强度上存在一定的差异。

Purification and analysis of AT-toxin Firstly, we detected the components of protein and nonprotein, the results showed that AT-toxin is a nonprotein. We have carried out the liquid chromatogram analysis of the toxin. The result indicates that there are many components in this toxin and there are four kinds of components which content is more than others. At last, we have the experiment of separating the cpmpositions with the silica gel and we get four components which are active.

AT-毒素的初步纯化与分析为确定AT-毒素的组分，首先对毒素的蛋白质和非蛋白质组分的毒性进行了测定，结果表明：AT-毒素活性组分为非蛋白质；对毒素进行硅胶G薄层层析，初步分离得到10种组分，其中四种组分具有活性：组分Ⅵ、Ⅶ、Ⅷ、Ⅸ，初步说明了该毒素含有多种组分；进一步对该毒素进行液相色谱分析，也发现该毒素含多种组分，其中四种组分峰高和峰面积较大，含量较高；其致毒活性可能是由这些组分协同作用的结果，它们之间可能存在正协同或负协同作用关系。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

The main reasons were that the algae and organism in the water decreased the ability of removing pollutants by coagulations, the algae which grown again in the open sedimentation tank weakened the removal effect of algae by coagulation; because of the accumulation of algae and algae toxin in the filtration tank, the concentration of algae toxin in the effluent increased; by the function of fluid chlorine, the algae and organisms after the filtration will release microalgae toxin and produce a lot of byproduct such as microalgae toxin, sobomyl and so on, meanwhile, the chlorination produce considerable halide and halo-acetic acid and other disinfection byproduct.

主要表现在藻类和溶解性有机物的存在降低了混凝去除污染物的能力；藻类在露天平流沉淀池中再生长，抵消了混凝除藻效果；藻类和藻毒素在滤池中积累，导致滤后藻毒素升高；穿透滤池的藻类和有机物在液氯作用下，释放微囊藻毒素、土臭素和二甲基异冰片等胞内物质，同时生成数量可观的卤代烃和卤乙酸等消毒副产物。

For example,1889-1890 years, the people studies the diphtheria toxin and the tetanus infection toxins with the immunology technique, using it again later on to study the plant toxin, such as the castor toxin, the croton toxin and snake poison, spider poison within the animal toxins.

例如，1889～1890年，人们用免疫学技术研究白喉毒素和破伤风毒素，随后又用它来研究植物毒素，如蓖麻毒素、巴豆毒素和动物毒素中的蛇毒、蜘蛛毒。

And to trojan and baleful software, the understanding of lucky star and strange tiger is different also, insist to be mixed with safe bodyguard all the time to strange tiger a variety of means such as the software that reduce toxin handle trojan program and virus, lucky star thinks the trojan is a kind of virus, instead trojan and turn over virus to be mixed in technical principle intercept and capture, there are any distinctions on flow of analysis, processing, is to persuade an user to stay, strange tiger with " of a year of operating period, the component reducing toxin that functional course cuts out " pretend to be " the permanent and free software that reduce toxin " will cheat an user, it is a crammer.

而对于木马和恶意软件，瑞星与奇虎的认识也不同，对于奇虎一直坚持用安全卫士和杀毒软件等多种方式处理木马程序以及病毒，瑞星认为木马是病毒的一种，反木马和反病毒在技术原理和截获、分析、处理流程上没有任何区别，而正是为了挽留用户，奇虎便以"一年使用期的、功能经过裁剪的杀毒组件"冒充"永久免费的杀毒软件"来欺骗用户，是一个谎言。

The traditional software that reduce toxin is with the feature code means reduces toxin, diagnostic code takes virus example former course namely, put forward a paragraph of of similar dactylogram unique code in the program, add in the software that reduce toxin, scan next all files, if have yard of this paragraph of feature, it is virus, can be killed.

传统杀毒软件都是以特征码方式杀毒，特征码即把病毒样本原程序拿到，提出程序中类似指纹的一段独一无二的代码，加到杀毒软件中，然后扫描所有文件，如果有这段特征码，就是病毒，即可被杀掉。

The experiment identified weed control spectrum of toxin and sensitivity to main crops. The result showed that dayflower and goosefoot were most sensitive to toxin with injury response of level 4, followed by redroot pigweed and eragrostioid with injury response of level 3 and level 2, respectively. Among the main crops, corn and sorghum were susceptible to toxin while wheat, soybean and peanut were insensitive.

粗毒素杀草谱和对作物安全性测试结果为：鸭跖草和藜对毒素最敏感，伤害反应为4级；反枝苋和虎尾草伤害反应分别为3级和1级；玉米和高粱对粗毒素敏感，小麦、大豆和花生对粗毒素不敏感。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。